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AA Sequence of recombinant protein: RVTKDAETEF MMSKLPLENP VLLDRFHATS ADCCISYTPR SIPCSLLESY FETNSECSKP GVIFLTKKGR RFCANPSDKQ VQVCMRMLKL DTRIKTRKN.
Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human MIP-3 (CCL23). Anti-Human MIP-3 (CCL23)-specific antibody was purified by affinity chromatography employing an immobilized Human MIP-3 (CCL23) matrix.
Sandwich ELISA: To detect Human MIP-3 (CCL23) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human MIP-3 (CCL23) (500-P124Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human MIP-3 (CCL23).
Western Blot: To detect Human MIP-3 (CCL23) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human MIP-3 (CCL23) is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
This gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity on resting T lymphocytes and monocytes, lower activity on neutrophils and no activity on activated T lymphocytes. The protein is also a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. In addition, the product of this gene is a potent agonist of the chemokine (C-C motif) receptor 1. Alternative splicing results in multiple transcript variants that encode different isoforms.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: C-C motif chemokine 23; C6 beta-chemokine; chemokine (C-C motif) ligand 23; Ck beta 8-1; CK-beta-8; CKB-8; Macrophage inflammatory protein 3; MIP-3; MIP-3CK-BETA-8; MPIF-1; MPIF-1Ckb-8-1; Myeloid progenitor inhibitory factor 1; small inducible cytokine subfamily A (Cys-Cys), member 23; Small-inducible cytokine A23
基因别名: CCL23; CK-BETA-8; Ckb-8; Ckb-8-1; CKb8; hmrp-2a; MIP-3; MIP3; MPIF-1; MPIF1; SCYA23
UniProt ID: (Human) P55773
Entrez Gene ID: (Human) 6368