Invitrogen

CCL5 (RANTES) Monoclonal Antibody, PeproTech®

CCL5 (RANTES) Antibody in Western Blot (WB)

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CCL5 (RANTES) Antibody (500-M75-500UG) in WB

Western Blot: To detect Human RANTES (CCL5) by Western Blot analysis CCL5 (RANTES) Monoclonal Antibody (Product # 500-M75-500UG) can be used at a concentration of 0.5-1.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human RANTES (CCL5) is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.

CCL5 (RANTES) Antibody in Immunohistochemistry (IHC)

CCL5 (RANTES) Antibody in Neutralization (Neu)

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产品信息

500-M75-500UG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-1.0 µg/mL

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免疫组化 (IHC)

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酶联免疫吸附实验 (ELISA)

2.0-4.0 µg/mL

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中和实验 (Neu)

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体外检测 (IV)

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免疫染色 (IS)

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse

分类

Monoclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human RANTES (CCL5)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2929127

产品详细信息

AA Sequence of recombinant protein: SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS.

Preparation: Produced in BALB/c mice immunized with highly pure Recombinant Human RANTES (CCL5). Anti-Human RANTES (CCL5)-specific antibody was purified from ascites fluid by Protein A affinity chromatography.

Sandwich ELISA: Assuming 100 µL/well, a concentration of 2.0-4.0 µg/mL of this antibody will detect at least 500 pg/mL of Recombinant Human RANTES (CCL5) when used with PeproTech Biotinylated Antigen Affinity Purified Anti-Human RANTES (CCL5) (500-P36BT) as the detection antibody at a concentration of approximately 0.5-1.0 µg/mL.

Western Blot: To detect Human RANTES (CCL5) by Western Blot analysis this antibody can be used at a concentration of 0.5-1.0 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human RANTES (CCL5) is 0.25-2.0 ng/lane, under reducing conditions and 0.25-0.5 ng/lane, under non-reducing conditions.

靶标信息

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Tumor Cell-Autonomous Pro-Metastatic Activities of PD-L1 in Human Breast Cancer Are Mediated by PD-L1-S283 and Chemokine Axes.

Cancers

Erlichman N,Baram T,Meshel T,Morein D,Da'adoosh B,Ben-Baruch A

500-M75 was used in Enzyme-linked immunosorbent assay to investigate the role of WT-PD-L1 in triple-negative breast cancer.

Fri Feb 18 00:00:00 UTC 2022

Notch-Mediated Tumor-Stroma-Inflammation Networks Promote Invasive Properties and CXCL8 Expression in Triple-Negative Breast Cancer.

Frontiers in immunology

Liubomirski Y,Lerrer S,Meshel T,Morein D,Rubinstein-Achiasaf L,Sprinzak D,Wiemann S,Körner C,Ehrlich M,Ben-Baruch A

500-M75 was used in Enzyme-linked immunosorbent assay to investigate pro-metastatic chemokines in triple-negative breast cancer.

Thu Oct 01 00:00:00 UTC 2020

Tumor-Stroma-Inflammation Networks Promote Pro-metastatic Chemokines and Aggressiveness Characteristics in Triple-Negative Breast Cancer.

Frontiers in immunology

Liubomirski Y,Lerrer S,Meshel T,Rubinstein-Achiasaf L,Morein D,Wiemann S,Körner C,Ben-Baruch A

500-M75 was used in Enzyme-linked immunosorbent assay to study the impact of tumor-stroma-inflammation networks on pro-metastatic phenotypes in triple-negative breast cancer (TNBC) to identify a novel tumor-stroma-inflammation networks that may promote TNBC aggressiveness by increasing the pro-malignancy potential of the TME and of the tumor cells themselves, and reveal key roles for CXCL8 in mediating these metastasis-promoting activities.

Mon Aug 10 00:00:00 UTC 2020

Lactate-activated macrophages induced aerobic glycolysis and epithelial-mesenchymal transition in breast cancer by regulation of CCL5-CCR5 axis: a positive metabolic feedback loop.

Oncotarget

Lin S,Sun L,Lyu X,Ai X,Du D,Su N,Li H,Zhang L,Yu J,Yuan S

Published figure using CCL5 (RANTES) monoclonal antibody (Product # 500-M75-500UG) in Neutralization

Fri Dec 15 00:00:00 UTC 2017

Regulation of the inflammatory profile of stromal cells in human breast cancer: prominent roles for TNF-α and the NF-κB pathway.

Stem cell research & therapy

Katanov C,Lerrer S,Liubomirski Y,Leider-Trejo L,Meshel T,Bar J,Feniger-Barish R,Kamer I,Soria-Artzi G,Kahani H,Banerjee D,Ben-Baruch A

500-M75 was used in Enzyme-linked immunosorbent assay to study the impact of tumour necrosis factor α and interleukin 1β on the inflammatory phenotype of cancer-associated fibroblasts and mesenchymal stem cells.

Fri May 01 00:00:00 UTC 2015

Tumor-promoting circuits that regulate a cancer-related chemokine cluster: dominance of inflammatory mediators over oncogenic alterations.

Cancers

Leibovich-Rivkin T,Buganim Y,Solomon H,Meshel T,Rotter V,Ben-Baruch A

500-M75 was used in Enzyme-linked immunosorbent assay to use an unbiased model system to propose that inflammatory mediators of the tumor milieu have dominating roles over oncogenic modifications in dictating the expression of a pro-malignancy chemokine readout.

Fri Jan 20 00:00:00 UTC 2012

Inflammatory mediators in breast cancer: coordinated expression of TNFα & IL-1β with CCL2 & CCL5 and effects on epithelial-to-mesenchymal transition.

BMC cancer

Soria G,Ofri-Shahak M,Haas I,Yaal-Hahoshen N,Leider-Trejo L,Leibovich-Rivkin T,Weitzenfeld P,Meshel T,Shabtai E,Gutman M,Ben-Baruch A

Published figure using CCL5 (RANTES) monoclonal antibody (Product # 500-M75-500UG) in Immunohistochemistry

Tue Apr 12 00:00:00 UTC 2011

Inflammatory mediators in breast cancer: coordinated expression of TNFα & IL-1β with CCL2 & CCL5 and effects on epithelial-to-mesenchymal transition.

BMC cancer

Soria G,Ofri-Shahak M,Haas I,Yaal-Hahoshen N,Leider-Trejo L,Leibovich-Rivkin T,Weitzenfeld P,Meshel T,Shabtai E,Gutman M,Ben-Baruch A

Published figure using CCL5 (RANTES) monoclonal antibody (Product # 500-M75-500UG) in Immunohistochemistry

Tue Apr 12 00:00:00 UTC 2011

Inflammatory mediators in breast cancer: coordinated expression of TNFα & IL-1β with CCL2 & CCL5 and effects on epithelial-to-mesenchymal transition.

BMC cancer

Soria G,Ofri-Shahak M,Haas I,Yaal-Hahoshen N,Leider-Trejo L,Leibovich-Rivkin T,Weitzenfeld P,Meshel T,Shabtai E,Gutman M,Ben-Baruch A

Published figure using CCL5 (RANTES) monoclonal antibody (Product # 500-M75-500UG) in Immunohistochemistry

Tue Apr 12 00:00:00 UTC 2011

The chemokine CCL5 as a potential prognostic factor predicting disease progression in stage II breast cancer patients.

Clinical cancer research : an official journal of the American Association for Cancer Research

Yaal-Hahoshen N,Shina S,Leider-Trejo L,Barnea I,Shabtai EL,Azenshtein E,Greenberg I,Keydar I,Ben-Baruch A

Tue Aug 01 00:00:00 UTC 2006

The CC chemokine RANTES in breast carcinoma progression: regulation of expression and potential mechanisms of promalignant activity.

Cancer research

Azenshtein E,Luboshits G,Shina S,Neumark E,Shahbazian D,Weil M,Wigler N,Keydar I,Ben-Baruch A

Fri Feb 15 00:00:00 UTC 2002

Elevated expression of the CC chemokine regulated on activation, normal T cell expressed and secreted (RANTES) in advanced breast carcinoma.

Cancer research

Luboshits G,Shina S,Kaplan O,Engelberg S,Nass D,Lifshitz-Mercer B,Chaitchik S,Keydar I,Ben-Baruch A

Wed Sep 15 00:00:00 UTC 1999

Elevated expression of the CC chemokine regulated on activation, normal T cell expressed and secreted (RANTES) in advanced breast carcinoma.

Cancer research

Luboshits G,Shina S,Kaplan O,Engelberg S,Nass D,Lifshitz-Mercer B,Chaitchik S,Keydar I,Ben-Baruch A

Wed Sep 15 00:00:00 UTC 1999

Immunohistochemical study of the beta-chemokine receptors CCR3 and CCR5 and their ligands in normal and Alzheimer's disease brains.

The American journal of pathology

Xia MQ,Qin SX,Wu LJ,Mackay CR,Hyman BT

Wed Jul 01 00:00:00 UTC 1998

Immunohistochemical study of the beta-chemokine receptors CCR3 and CCR5 and their ligands in normal and Alzheimer's disease brains.

The American journal of pathology

Xia MQ,Qin SX,Wu LJ,Mackay CR,Hyman BT

Wed Jul 01 00:00:00 UTC 1998

生物信息学

蛋白别名: beta-chemokine RANTES; C-C motif chemokine 5; chemokine (C-C motif) ligand 5; EoCP; Eosinophil chemotactic cytokine; MGC17164; OTTHUMP00000197106; regulated upon activation, normally T-expressed, and presumably secreted; SIS δ; SIS-delta; SISδ; small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; T-cell-specific protein RANTES; TCP228

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基因别名: CCL5; D17S136E; eoCP; RANTES; SCYA5; SIS-delta; SISd; TCP228

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UniProt ID: (Human) P13501

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Entrez Gene ID: (Human) 6352

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功能