Invitrogen

CD105 Monoclonal Antibody (209701)

2 篇参考文献

查看其他96个CD105 (Endoglin)抗体

问题与答案

问题与答案

问题与答案

Additional Information:

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抗体检测数据 (6)

CD105 Antibody in Immunocytochemistry (ICC/IF)

CD105 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 6

CD105 Antibody (MA5-23894) in ICC/IF

Immunocytochemistry analysis of CD105 in immersion fixed mouse mesenchymal stem cells. Samples were incubated in CD105 monoclonal antibody (Product # MA5-23894) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

CD105 Antibody in Immunocytochemistry (ICC/IF)

CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

CD105 Antibody in Immunohistochemistry (Frozen) (IHC (F))

CD105 Antibody in Immunohistochemistry (Frozen) (IHC (F))

CD105 Antibody in Flow Cytometry (Flow)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

CD105 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of CD105 in immersion fixed mouse mesenchymal stem cells. Samples were incubated in CD105 monoclonal antibody (Product # MA5-23894) using a dilution of 10 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CD105 in immersion fixed frozen sections of mouse embryo. Samples were incubated with CD105 Monoclonal Antibody (Product # MA5-23894) using a dilution of 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG HRP Secondary Antibody. Tissue was stained with DAB (brown) and counterstained with hematoxylin (blue).

CD105 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of CD105 in immersion fixed frozen sections of mouse embryo. Samples were incubated with CD105 Monoclonal Antibody (Product # MA5-23894) using a dilution of 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG HRP Secondary Antibody. Tissue was stained with DAB (brown) and counterstained with hematoxylin (blue).

CD105 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemical analysis of CD105 in immersion fixed frozen sections of mouse embryo (13.5 d.p.c.). Samples were incubated with CD105 monoclonal antibody (Product # MA5-23894) using a dilution of 10 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells of the developing hindlimb. Image depicts staining at 40X magnification.

CD105 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemical analysis of CD105 in immersion fixed frozen sections of mouse embryo (13.5 d.p.c.). Samples were incubated with CD105 monoclonal antibody (Product # MA5-23894) using a dilution of 10 µg/mL overnight at 4 °C followed by NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells of the developing hindlimb. Image depicts staining at 10X magnification.

CD105 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of CD105 in MS-1 cells (filled histogram) vs isotype control antibody (open histogram) using CD105 Monoclonal Antibody (Product # MA5-23894). Followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody.

Immunogen image

CD105 Monoclonal Antibody (209701)

Immunogen Image CD105 Monoclonal Antibody (209701)

产品信息

MA5-23894

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

-

免疫组化 (IHC)

-

查看2篇已发表文章 2篇已发表文章

免疫组化（石蜡） (IHC (P))

8-25 µg/mL

-

免疫组化（冰冻） (IHC (F))

8-25 µg/mL

-

免疫细胞化学 (ICC/IF)

8-25 µg/mL

-

流式细胞分析 (Flow)

0.25 µg per million cells

-

产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

209701

抗原

Mouse myeloma cell line NS0-derived recombinant mouse Endoglin Glu21-Gly581 (predicted)

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.5 mg/mL

100 µg

纯化类型

Protein A/G

保存液

PBS with 5% trehalose

内含物

No Preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

Ambient (domestic); Wet ice (international)

产品详细信息

In Western blots, this antibody shows 100% cross-reactivity with recombinant human Endoglin.

Reconstitute at 0.5 mg/mL in sterile PBS.

靶标信息

CD105 or Endoglin is a Type I transmembrane protein, which is highly expressed on human vascular endothelial cells. It exists on an O- and N-glycosylated homodimer. Up regulation of endoglin expression has been demonstrated in tumor vasculature and proliferating cells, suggesting that it is a proliferation associated endothelial marker. CD105 binds to TGF beta 1 and 3 with high affinity but not to TGF beta 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: cell surface MJ7/18 antigen; co-receptor; ORW; TGF-beta receptor III homolog; transmembrane glycoprotein; unnamed protein product

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基因别名: CD105; Endo; S-endoglin

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UniProt ID: (Mouse) Q63961

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Entrez Gene ID: (Mouse) 13805

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type II transforming growth factor beta receptor binding protein binding galactose binding glycosaminoglycan binding coreceptor activity receptor activator activity type I transforming growth factor beta receptor binding BMP binding identical protein binding protein homodimerization activity transforming growth factor beta binding

negative regulation of transcription from RNA polymerase II promoter angiogenesis patterning of blood vessels vasculogenesis response to hypoxia positive regulation of protein phosphorylation negative regulation of endothelial cell proliferation heart looping sprouting angiogenesis positive regulation of systemic arterial blood pressure outflow tract septum morphogenesis epithelial to mesenchymal transition involved in endocardial cushion formation endocardial cushion morphogenesis cardiac ventricle morphogenesis cardiac atrium morphogenesis ventricular trabecula myocardium morphogenesis cell migration involved in endocardial cushion formation regulation of transcription, DNA-templated transforming growth factor beta receptor signaling pathway heart development positive regulation of gene expression negative regulation of gene expression regulation of cardiac muscle cell apoptotic process cell migration regulation of transforming growth factor beta receptor signaling pathway central nervous system vasculogenesis extracellular matrix disassembly negative regulation of cell migration positive regulation of BMP signaling pathway positive regulation of collagen biosynthetic process dorsal aorta morphogenesis wound healing positive regulation of angiogenesis positive regulation of transcription from RNA polymerase II promoter smooth muscle tissue development artery morphogenesis venous blood vessel morphogenesis cell motility positive regulation of protein kinase B signaling atrial cardiac muscle tissue morphogenesis cell chemotaxis positive regulation of SMAD protein import into nucleus negative regulation of SMAD protein import into nucleus extracellular matrix constituent secretion detection of hypoxia endocardial cushion to mesenchymal transition vascular smooth muscle cell development positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation positive regulation of vascular smooth muscle cell differentiation cardiac jelly development atrioventricular canal morphogenesis regulation of cardiac neural crest cell migration involved in outflow tract morphogenesis regulation of cell proliferation involved in heart morphogenesis