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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫组化（冰冻） (IHC (F)) |
Assay-dependent | - |
流式细胞分析 (Flow) |
Neat | 查看1篇已发表文章 1篇已发表文章 |
免疫沉淀 (IP) |
Assay-dependent | - |
| 产品规格 | |
|---|---|
种属反应 |
Bovine, Dog, Cat, Goat, Mustelid, Mink, Pig |
已发表种属 |
Dog |
宿主/亚型 |
Mouse / IgG1 |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
CA16.3E10 |
抗原 |
Affinity purified beta-2 integrins from splenic lysate |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
Conc. Not Determined |
保存液 |
tissue culture supernatant diluted in PBS |
内含物 |
<0.1% sodium azide |
保存条件 |
4°C or -20°C if preferred |
RRID |
AB_2538103 |
The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Dog CD11b antibody, clone CA16.3E10 is a monoclonal antibody recognising the canine CD11b cell surface antigen, a member of the alpha integrin family.
CD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: antigen CD11b (p170); macrophage antigen alpha polypeptide; integrin alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); leukocyte integrin alpha-M chain; Mac-1 alpha (Mac1A); MGC117044
基因别名: CD11B
Entrez Gene ID: (Pig) 397459, (Goat) 102180100, (Bovine) 407124, (Dog) 489928
分子生物学功能:
integrin
