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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
流式细胞分析 (Flow) |
5 µL (0.5 µg)/test | 查看9篇已发表文章 9篇已发表文章 |
| 产品规格 | |
|---|---|
种属反应 |
Human |
已发表种属 |
Human, Mouse |
宿主/亚型 |
Mouse / IgG1, kappa |
推荐的同型对照 |
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™ |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
3.9 |
偶联物 |
eFluor™ 450
|
激发/发射光谱 |
查看光谱
|
形式 |
Liquid |
浓度 |
5 µL/Test |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA |
内含物 |
0.09% sodium azide |
保存条件 |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_11220072 |
Description: The 3.9 monoclonal antibody reacts with human CD11c, the 150 kDa integrin alpha X chain. CD11c non-covalently associates with beta 2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions.
Applications Reported: This 3.9 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 3.9 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.
Filtration: 0.2 µm post-manufacturing filtered.
CD11, along with CD18, form a heterodimer adhesion molecule. In particular, CD11 is composed of CD11a, CD11b and CD11c. CD11a is a leukocyte marker that is expressed in B and T lymphocytes, macrophages, monocytes, neutrophils, basophils and eosinophils. CD11b is primarily expressed by monocytes, macrophages, natural killer cells, some B and T-cells, and granulocytes. CD11c is expressed in monocytes, macrophages, natural killer cells, some granulocytes and less so in a subset of lymphocytes.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
蛋白别名: CD11 antigen-like family member C; CD11c; complement component 3 receptor 4 subunit; integrin alpha X; Integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); integrin, alpha X (complement component 3 receptor 4 subunit); Leu M5; leu M5, alpha subunit; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; leukocyte surface antigen p150,95, alpha subunit; myeloid membrane antigen, alpha subunit; p150 95 integrin alpha chain
基因别名: CD11C; ITGAX; SLEB6
UniProt ID: (Human) P20702
Entrez Gene ID: (Human) 3687
分子生物学功能:
integrin
