Invitrogen

CD123 Monoclonal Antibody (6H6), Super Bright™ 436, eBioscience™

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CD123 Antibody (62-1239-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD85g (ILT7) APC (Product # 17-5179-42) and Mouse IgG1 K Isotype Control Super Bright 436 (Product # 62-4714-82) (left) or Anti-Human CD123 Super Bright 436 (right). Cells in the monocyte gate were used for analysis.

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产品信息

62-1239-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/Test

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产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

6H6

偶联物

Super Bright™ 436 Super Bright™ 436 Super Bright™ 436

激发/发射光谱

413/431 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS with BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2662727

产品详细信息

The 6H6 monoclonal antibody reacts with human CD123, the alpha chain of the IL-3 receptor. This 60-70 kDa transmembrane protein binds to IL-3 with low affinity by itself, and when associated with CD131 (common beta chain) binds IL-3 with high affinity. CD123 is expressed by myeloid precursors, macrophages, dendritic cells, mast cells, basophils, and megakaryocytes.

This 6H6 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 2-8°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorochrome performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

靶标信息

IL-3Ra (Interleukin-3 receptor subunit alpha) belongs to the type I cytokine receptor family and is a heterodimer with a unique alpha chain paired with the common beta (beta c or CDw131) subunit. IL-3Ra is responsible for the transmission of signal of Interleukin-3. IL-3Ra and IL-3Rb chain heterodimerization is induced by Interleukin-3, which is required for receptor activation but not high affinity binding. IL-3Ra is also reported that IL-3Ra subunits are overexpressed on acute myeloid leukemia (AML) blasts compared with normal hematopoietic cells and are thus potential targets for novel therapeutic agents.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

High-dimensional immune cell profiling of cerebrospinal fluid from patients with metastatic breast cancer and leptomeningeal disease.

NPJ breast cancer

Im KW,Huppert LA,Malevanchik L,Rugo HS,Combes AJ,Campbell MJ,Krummel MF,Melisko ME

62-1239-42 was used in Flow cytometry/Cell sorting to suggest that patients with LMD may have lower overall immune infiltrates than patients without LMD, suggesting a more permissive CSF immune microenvironment but a higher frequency of partially exhausted CD8+ T cells, which may offer an important therapeutic target.

Fri Apr 07 00:00:00 EDT 2023

Impaired IL-23-dependent induction of IFN-γ underlies mycobacterial disease in patients with inherited TYK2 deficiency.

The Journal of experimental medicine

Ogishi M,Arias AA,Yang R,Han JE,Zhang P,Rinchai D,Halpern J,Mulwa J,Keating N,Chrabieh M,Lainé C,Seeleuthner Y,Ramírez-Alejo N,Nekooie-Marnany N,Guennoun A,Muller-Fleckenstein I,Fleckenstein B,Kilic SS,Minegishi Y,Ehl S,Kaiser-Labusch P,Kendir-Demirkol Y,Rozenberg F,Errami A,Zhang SY,Zhang Q,Bohlen J,Philippot Q,Puel A,Jouanguy E,Pourmoghaddas Z,Bakhtiar S,Willasch AM,Horneff G,Llanora G,Shek LP,Chai LYA,Tay SH,Rahimi HH,Mahdaviani SA,Nepesov S,Bousfiha AA,Erdeniz EH,Karbuz A,Marr N,Navarrete C,Adeli M,Hammarstrom L,Abolhassani H,Parvaneh N,Al Muhsen S,Alosaimi MF,Alsohime F,Nourizadeh M,Moin M,Arnaout R,Alshareef S,El-Baghdadi J,Genel F,Sherkat R,Kiykim A,Yücel E,Keles S,Bustamante J,Abel L,Casanova JL,Boisson-Dupuis S

62-1239-42 was used in Flow cytometry/Cell sorting to show that partial TYK2 deficiency across signaling pathways, or rare or common partial TYK2 deficiency specific for IL-23 signaling.

Mon Oct 03 00:00:00 EDT 2022

Unsupervised Analysis of Flow Cytometry Data in a Clinical Setting Captures Cell Diversity and Allows Population Discovery.

Frontiers in immunology

Baumgaertner P,Sankar M,Herrera F,Benedetti F,Barras D,Thierry AC,Dangaj D,Kandalaft LE,Coukos G,Xenarios I,Guex N,Harari A

Published figure using CD123 monoclonal antibody (Product # 62-1239-42) in Flow Cytometry

Tue Sep 21 00:00:00 EDT 2021

Mucosal-associated invariant T cell responses differ by sex in COVID-19.

Med (New York, N.Y.)

Yu C,Littleton S,Giroux NS,Mathew R,Ding S,Kalnitsky J,Yang Y,Petzold E,Chung HA,Rivera GO,Rotstein T,Xi R,Ko ER,Tsalik EL,Sempowski GD,Denny TN,Burke TW,McClain MT,Woods CW,Shen X,Saban DR

62-1239-42 was used in Flow Cytometry to reveal a pronounced reduction of circulating mucosal-associated invariant T (MAIT) cells in infected females.

Fri Jun 11 00:00:00 EDT 2021

Single-Cell Analyses Reveal Megakaryocyte-Biased Hematopoiesis in Myelofibrosis and Identify Mutant Clone-Specific Targets.

Molecular cell

Psaila B,Wang G,Rodriguez-Meira A,Li R,Heuston EF,Murphy L,Yee D,Hitchcock IS,Sousos N,O'Sullivan J,Anderson S,Senis YA,Weinberg OK,Calicchio ML,Iskander D,Royston D,Milojkovic D,Roberts I,Bodine DM,Thongjuea S,Mead AJ

Published figure using CD123 monoclonal antibody (Product # 62-1239-42) in Flow Cytometry

Thu May 07 00:00:00 EDT 2020

Systems Biology Methods Applied to Blood and Tissue for a Comprehensive Analysis of Immune Response to Hepatitis B Vaccine in Adults.

Frontiers in immunology

Ben-Othman R,Cai B,Liu AC,Varankovich N,He D,Blimkie TM,Lee AH,Gill EE,Novotny M,Aevermann B,Drissler S,Shannon CP,McCann S,Marty K,Bjornson G,Edgar RD,Lin DTS,Gladish N,Maclsaac J,Amenyogbe N,Chan Q,Llibre A,Collin J,Landais E,Le K,Reiss SM,Koff WC,Havenar-Daughton C,Heran M,Sangha B,Walt D,Krajden M,Crotty S,Sok D,Briney B,Burton DR,Duffy D,Foster LJ,Mohn WW,Kobor MS,Tebbutt SJ,Brinkman RR,Scheuermann RH,Hancock REW,Kollmann TR,Sadarangani M

Published figure using CD123 monoclonal antibody (Product # 62-1239-42) in Flow Cytometry

Mon Jun 21 00:00:00 EDT 2021

Neutrophils Inhibit Synthesis of Mineralized Extracellular Matrix by Human Bone Marrow-Derived Stromal Cells In Vitro.

Frontiers in immunology

Bastian OW,Croes M,Alblas J,Koenderman L,Leenen LPH,Blokhuis TJ

Published figure using CD123 monoclonal antibody (Product # 62-1239-42) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2019

Malaria-induced NLRP12/NLRP3-dependent caspase-1 activation mediates inflammation and hypersensitivity to bacterial superinfection.

PLoS pathogens

Ataide MA,Andrade WA,Zamboni DS,Wang D,Souza Mdo C,Franklin BS,Elian S,Martins FS,Pereira D,Reed G,Fitzgerald KA,Golenbock DT,Gazzinelli RT

Published figure using CD123 monoclonal antibody (Product # 62-1239-42) in Flow Cytometry

Wed Jan 01 00:00:00 EST 2014

Frailty in old age is associated with decreased interleukin-12/23 production in response to toll-like receptor ligation.

PloS one

Compté N,Zouaoui Boudjeltia K,Vanhaeverbeek M,De Breucker S,Tassignon J,Trelcat A,Pepersack T,Goriely S

Published figure using CD123 monoclonal antibody (Product # 62-1239-42) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2014

Irreversible loss of pDCs by apoptosis during early HIV infection may be a critical determinant of immune dysfunction.

Viral immunology

Meera S,Madhuri T,Manisha G,Ramesh P

Published figure using CD123 monoclonal antibody (Product # 62-1239-42) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2010

生物信息学

蛋白别名: CD123; CD123 antigen; IL-3 receptor alpha SP2 isoform; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; interleukin 3 receptor, alpha (low affinity); Interleukin-3 receptor subunit alpha

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基因别名: CD123; hIL-3Ra; IL3R; IL3RA; IL3RAY; IL3RX; IL3RY

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UniProt ID: (Human) P26951

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Entrez Gene ID: (Human) 3563

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功能

protein tyrosine kinase activity interleukin-3 receptor activity Ras guanyl-nucleotide exchange factor activity transmembrane signal receptor

参与通路

MAPK cascade peptidyl-tyrosine phosphorylation cellular response to interleukin-3 interleukin-3-mediated signaling pathway positive regulation of GTPase activity