Invitrogen

CD127 Monoclonal Antibody (eBioRDR5), PE-Cyanine5, eBioscience™

图: 1 / 15

CD127 Antibody (15-1278-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD3 FITC (Product # 11-0037-42) and Mouse IgG1 K Isotype Control PE-Cyanine5 (Product # 15-4714-81) (left) or Anti-Human CD127 PE-Cyanine5 (right).Cells in the lymphocyte gate were used for analysis.

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产品信息

15-1278-42

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

查看1篇已发表文章 1篇已发表文章

流式细胞分析 (Flow)

5 µL (0.125 µg)/test

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产品规格

种属反应

Human

已发表种属

Human, Non-human primate, Rhesus monkey

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioRDR5

偶联物

PE-Cyanine5 PE-Cyanine5 PE-Cyanine5

激发/发射光谱

568/666 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2043801

产品详细信息

Description: The eBioRDR5 monoclonal antibody reacts with human CD127 (Interleukin-7 Receptor alpha). CD127 complexes with CD132, also known as the common gamma chain (gamma c), to form the multi-functional IL-7 receptor (IL-7R). CD127 is a type I glycoprotein with a molecular weight of 75-80 kDa and is expressed by immature B cells through the early pre-B stage, by thymocytes during several stages of their development, and on most mature T cells, with transient down-regulation upon activation. Binding of IL-7 results in signal transduction which occurs through several tyrosine kinase pathways including the Jak/STAT pathway. IL-7 is indispensible for the development of lymphocytes, and the control of homeostatic proliferation of T-cells in the periphery. In addition, IL-7R signaling is know to be involved in the regulation of T cell receptor (TCR) locus rearrangement in gamma delta T cells.

Interestingly, recently it has been demonstrated that CD127 expression is down-regulated on CD4+CD25+ regulatory T cells (T regs). While the co-expression of CD4 and CD25 has become widely used as an indicator of T regs, this method of identification may also include cells without suppressive activity. It has clearly been shown that CD4+CD25+ cells that have down-regulated the expression of CD127 are significantly more highly-enriched for the regulatory T population, as defined by expression of the T reg-specific transcription factor Foxp3 and the suppressive activity of these cells, in vitro.

Binding of the eBioRDR5 monoclonal antibody to PBMCs is blocked by pre-incubation of the cells with recombinant human IL-7 (Product # 14-1079-80).

Applications Reported: This eBioRDR5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioRDR5 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 667 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD127 (Interleukin-7, IL-7) is a glycoprotein involved in the regulation of lymphopoiesis. The response of cells to CD127 is dependent on the presence of the interleukin 7 receptor (IL7R); the active receptor is an alpha/gamma chain heterodimer. CD127 consists of an alpha chain and a gamma chain. The gamma(c) chain, which also associates with the interleukin-2 receptor, serves primarily to activate signal transduction by the IL7R complex, while the alpha chain of IL7R determines specific signaling events through its association with cytoplasmic signaling molecules. CD127 promotes the proliferation of precursor B cells, thymocytes, T cell progenitors, and mature CD4+ and CD8+ T cells. The biological effects of IL7 are mediated by the binding of IL7 to the specific cell surface receptor. Diseases associated with CD127 dysfunction include severe combined immunodeficiency and T cell negative/B cell negative/NK positive severe combined immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

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Hoang TN,Pino M,Boddapati AK,Viox EG,Starke CE,Upadhyay AA,Gumber S,Nekorchuk M,Busman-Sahay K,Strongin Z,Harper JL,Tharp GK,Pellegrini KL,Kirejczyk S,Zandi K,Tao S,Horton TR,Beagle EN,Mahar EA,Lee MYH,Cohen J,Jean SM,Wood JS,Connor-Stroud F,Stammen RL,Delmas OM,Wang S,Cooney KA,Sayegh MN,Wang L,Filev PD,Weiskopf D,Silvestri G,Waggoner J,Piantadosi A,Kasturi SP,Al-Shakhshir H,Ribeiro SP,Sekaly RP,Levit RD,Estes JD,Vanderford TH,Schinazi RF,Bosinger SE,Paiardini M

15-1278 was used in Flow cytometry/Cell sorting to investigate the immunologic and virologic efficacy of baricitinib in a rhesus macaque model of SARS-CoV-2 infection.

Thu Jan 21 00:00:00 EST 2021

Epinephrine Production in Th17 Cells and Experimental Autoimmune Encephalitis.

Frontiers in immunology

Yang P,Tian H,Zou YR,Chambon P,Ichinose H,Honig G,Diamond B,Kim SJ

Published figure using CD127 monoclonal antibody (Product # 15-1278-42) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

CTLA-4 and PD-1 dual blockade induces SIV reactivation without control of rebound after antiretroviral therapy interruption.

Nature medicine

Harper J,Gordon S,Chan CN,Wang H,Lindemuth E,Galardi C,Falcinelli SD,Raines SLM,Read JL,Nguyen K,McGary CS,Nekorchuk M,Busman-Sahay K,Schawalder J,King C,Pino M,Micci L,Cervasi B,Jean S,Sanderson A,Johns B,Koblansky AA,Amrine-Madsen H,Lifson J,Margolis DM,Silvestri G,Bar KJ,Favre D,Estes JD,Paiardini M

15-1278 was used in Flow cytometry/Cell sorting to demonstrate that PD-1, CTLA-4 and dual CTLA-4/PD-1 immune checkpoint blockade using monoclonal antibodies is well tolerated, with evidence of bioactivity in blood and lymph nodes.

Wed Apr 01 00:00:00 EDT 2020

Baricitinib treatment resolves lower airway inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques.

bioRxiv : the preprint server for biology

Hoang TN,Pino M,Boddapati AK,Viox EG,Starke CE,Upadhyay AA,Gumber S,Busman-Sahay K,Strongin Z,Harper JL,Tharp GK,Pellegrini KL,Kirejczyk S,Zandi K,Tao S,Horton TR,Beagle EN,Mahar EA,Lee MY,Cohen J,Jean SM,Wood JS,Connor-Stroud F,Stammen RL,Delmas OM,Wang S,Cooney KA,Sayegh MN,Wang L,Weiskopf D,Filev PD,Waggoner J,Piantadosi A,Kasturi SP,Al-Shakhshir H,Ribeiro SP,Sekaly RP,Levit RD,Estes JD,Vanderford TH,Schinazi RF,Bosinger SE,Paiardini M

15-1278 was used in Flow cytometry/Cell sorting to investigate the immunologic and virologic efficacy of baricitinib in a rhesus macaque model of SARS-CoV-2 infection.

Wed Sep 16 00:00:00 EDT 2020

Baricitinib treatment resolves lower airway inflammation and neutrophil recruitment in SARS-CoV-2-infected rhesus macaques.

bioRxiv : the preprint server for biology

15-1278 was used in Flow cytometry/Cell sorting to investigate the immunologic and virologic efficacy of baricitinib in a rhesus macaque model of SARS-CoV-2 infection.

Wed Sep 16 00:00:00 EDT 2020

Regulatory T cells as a biomarker for response to adalimumab in rheumatoid arthritis.

The Journal of allergy and clinical immunology

Nguyen DX,Cotton A,Attipoe L,Ciurtin C,Doré CJ,Ehrenstein MR

15-1278 was used in Flow cytometry/Cell sorting to determine whether adalimumab's ability to boost Treg cells in vitro using PBMCs before treatment would predict clinical response.

Sat Sep 01 00:00:00 EDT 2018

A probiotic modulates the microbiome and immunity in multiple sclerosis.

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Tankou SK,Regev K,Healy BC,Tjon E,Laghi L,Cox LM,Kivisäkk P,Pierre IV,Hrishikesh L,Gandhi R,Cook S,Glanz B,Stankiewicz J,Weiner HL

Published figure using CD127 monoclonal antibody (Product # 15-1278-42) in Flow Cytometry

Fri Jun 01 00:00:00 EDT 2018

Characterization of CD127^- CD25⁺⁺ Treg from human colostrum.

American journal of reproductive immunology (New York, N.Y. : 1989)

Cérbulo-Vázquez A,Hernández-Peláez G,Arriaga-Pizano LA,Bautista-Pérez P,Romero-Venado J,Flores-González JC,Figueroa-Damian R,Soriano-Becerril D,Mancilla-Herrera I

Published figure using CD127 monoclonal antibody (Product # 15-1278-42) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

The Effects of High Mobility Group Box-1 Protein on Peripheral Treg/Th17 Balance in Patients with Atherosclerosis.

Acta Cardiologica Sinica

Ding JW,Zhou T,Zheng XX,Wang XA,Tong XH,Luo CY,Zhang ZQ,Yu B

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Sat Sep 01 00:00:00 EDT 2018

Activation of miR-21-Regulated Pathways in Immune Aging Selects against Signatures Characteristic of Memory T Cells.

Cell reports

Kim C,Hu B,Jadhav RR,Jin J,Zhang H,Cavanagh MM,Akondy RS,Ahmed R,Weyand CM,Goronzy JJ

Published figure using CD127 monoclonal antibody (Product # 15-1278-42) in Flow Cytometry

Tue Nov 20 00:00:00 EST 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

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Sat Apr 01 00:00:00 EDT 2017

ATP Release from Chemotherapy-Treated Dying Leukemia Cells Elicits an Immune Suppressive Effect by Increasing Regulatory T Cells and Tolerogenic Dendritic Cells.

Frontiers in immunology

Lecciso M,Ocadlikova D,Sangaletti S,Trabanelli S,De Marchi E,Orioli E,Pegoraro A,Portararo P,Jandus C,Bontadini A,Redavid A,Salvestrini V,Romero P,Colombo MP,Di Virgilio F,Cavo M,Adinolfi E,Curti A

15-1278 was used in Flow cytometry/Cell sorting to study the effect of ATP release in acute myeloid lymphoma on the generation of an immune suppressive microenvironment.

Thu Mar 31 00:00:00 EDT 2022

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

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Tue Dec 26 00:00:00 EST 2017

Serial immunomonitoring of cancer patients receiving combined antagonistic anti-CD40 and chemotherapy reveals consistent and cyclical modulation of T cell and dendritic cell parameters.

BMC cancer

McDonnell AM,Cook A,Robinson BWS,Lake RA,Nowak AK

Published figure using CD127 monoclonal antibody (Product # 15-1278-42) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

Efficacy, durability, and response predictors of low-dose interleukin-2 therapy for chronic graft-versus-host disease.

Blood

Koreth J,Kim HT,Jones KT,Lange PB,Reynolds CG,Chammas MJ,Dusenbury K,Whangbo J,Nikiforow S,Alyea EP,Armand P,Cutler CS,Ho VT,Chen YB,Avigan D,Blazar BR,Antin JH,Ritz J,Soiffer RJ

15-1278 was used in Flow cytometry/Cell sorting to study the efficacy, durability, and response predictors of low-dose interleukin-2 therapy in the treatment of chronic graft-versus-host disease.

Thu Jul 07 00:00:00 EDT 2016

Anti-TNF drives regulatory T cell expansion by paradoxically promoting membrane TNF-TNF-RII binding in rheumatoid arthritis.

The Journal of experimental medicine

Nguyen DX,Ehrenstein MR

15-1278 was used in Flow cytometry/Cell sorting to investigate whether TNF boosts of inhibits regulatory T cells (T reg cells), showing that anti-TNF drives regulatory T cell expansion by promoting membrane TNF-TNF-RII binding, in rheumatoid arthritis.

Mon Jun 27 00:00:00 EDT 2016

The Distribution of Human Stem Cell-like Memory T Cell in Lung Cancer.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Hong H,Gu Y,Sheng SY,Lu CG,Zou JY,Wu CY

Published figure using CD127 monoclonal antibody (Product # 15-1278-42) in Flow Cytometry

Thu Jan 04 00:00:00 EST 2018

CMV-specific T cells generated from naïve T cells recognize atypical epitopes and may be protective in vivo.

Science translational medicine

Hanley PJ,Melenhorst JJ,Nikiforow S,Scheinberg P,Blaney JW,Demmler-Harrison G,Cruz CR,Lam S,Krance RA,Leung KS,Martinez CA,Liu H,Douek DC,Heslop HE,Rooney CM,Shpall EJ,Barrett AJ,Rodgers JR,Bollard CM

15-1278 was used in Flow cytometry/Cell sorting to suggest that naïve T cells from seronegative patients could be a source of cells for cytomegalovirus immunoprophylaxis.

Wed Apr 29 00:00:00 EDT 2015

Decreased T Follicular Regulatory Cell/T Follicular Helper Cell (TFH) in Simian Immunodeficiency Virus-Infected Rhesus Macaques May Contribute to Accumulation of TFH in Chronic Infection.

Journal of immunology (Baltimore, Md. : 1950)

Chowdhury A,Del Rio Estrada PM,Tharp GK,Trible RP,Amara RR,Chahroudi A,Reyes-Teran G,Bosinger SE,Silvestri G

15-1278 was used in Flow cytometry/Cell sorting to explore the regulation of HIV/SIV-associated T follicular helper cell expansion.

Thu Oct 01 00:00:00 EDT 2015

Improved Natural Killer cell activity and retained anti-tumor CD8(+) T cell responses contribute to the induction of a pathological complete response in HER2-positive breast cancer patients undergoing neoadjuvant chemotherapy.

Journal of translational medicine

Muraro E,Comaro E,Talamini R,Turchet E,Miolo G,Scalone S,Militello L,Lombardi D,Spazzapan S,Perin T,Massarut S,Crivellari D,Dolcetti R,Martorelli D

15-1278 was used in Flow cytometry/Cell sorting to indicate that maintenance of functional T cell responses against selected antigens and improvement of NK cell proficiency during NC are probably critical requirements for pCR induction.

Sat Jun 27 00:00:00 EDT 2015

Enhanced suppressor function of TIM-3+ FoxP3+ regulatory T cells.

European journal of immunology

Gautron AS,Dominguez-Villar M,de Marcken M,Hafler DA

Published figure using CD127 monoclonal antibody (Product # 15-1278-42) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2014

Highly heterogeneous, activated, and short-lived regulatory T cells during chronic filarial infection.

European journal of immunology

Metenou S,Coulibaly YI,Sturdevant D,Dolo H,Diallo AA,Soumaoro L,Coulibaly ME,Kanakabandi K,Porcella SF,Klion AD,Nutman TB

15-1278 was used in Flow cytometry/Cell sorting to study how in filarial infection, the expanded Treg-cell populations are heterogeneous, short-lived, activated, and express higher levels of molecules known to modulate immune responsiveness.

Tue Jul 01 00:00:00 EDT 2014

The number of CCR5 expressing CD4+ T lymphocytes is lower in HIV-infected long-term non-progressors with viral control compared to normal progressors: a cross-sectional study.

BMC infectious diseases

Meijerink H,Indrati AR,van Crevel R,Joosten I,Koenen H,van der Ven AJ

15-1278 was used in Flow cytometry/Cell sorting to determine levels of CCR5 and CXCR4 expression among CD4(+) and CD8(+) lymphocyte subsets in HIV-infected long-term non-progressors.

Sat Dec 13 00:00:00 EST 2014

Phase I study of alemtuzumab for therapy of steroid-refractory chronic graft-versus-host disease.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Nikiforow S,Kim HT,Bindra B,McDonough S,Glotzbecker B,Armand P,Koreth J,Ho VT,Alyea EP,Blazar BR,Ritz J,Soiffer RJ,Antin JH,Cutler CS

15-1278 was used in Flow cytometry/Cell sorting to indicate that therapy with alemtuzumab for steroid-refractory chronic graft-versus-host disease is tolerable with close attention to dosing.

Wed May 01 00:00:00 EDT 2013

Bortezomib-based graft-versus-host disease prophylaxis in HLA-mismatched unrelated donor transplantation.

Journal of clinical oncology : official journal of the American Society of Clinical Oncology

Koreth J,Stevenson KE,Kim HT,McDonough SM,Bindra B,Armand P,Ho VT,Cutler C,Blazar BR,Antin JH,Soiffer RJ,Ritz J,Alyea EP

15-1278 was used in Flow cytometry/Cell sorting to show that a novel, short-course, bortezomib-based GVHD regimen is safe and efficacious in HLA-mismatched unrelated donor reduced-intensity conditioning HSCT.

Mon Sep 10 00:00:00 EDT 2012

Interleukin-2 and regulatory T cells in graft-versus-host disease.

The New England journal of medicine

Koreth J,Matsuoka K,Kim HT,McDonough SM,Bindra B,Alyea EP,Armand P,Cutler C,Ho VT,Treister NS,Bienfang DC,Prasad S,Tzachanis D,Joyce RM,Avigan DE,Antin JH,Ritz J,Soiffer RJ

15-1278 was used in Flow cytometry/Cell sorting to assess whether low-dose interleukin-2 could preferentially enhance Treg cells in vivo and suppress clinical manifestations of chronic graft-versus-host disease.

Thu Dec 01 00:00:00 EST 2011

Bet v 1-specific T-cell receptor/forkhead box protein 3 transgenic T cells suppress Bet v 1-specific T-cell effector function in an activation-dependent manner.

The Journal of allergy and clinical immunology

Schmetterer KG,Haiderer D,Leb-Reichl VM,Neunkirchner A,Jahn-Schmid B,Küng HJ,Schuch K,Steinberger P,Bohle B,Pickl WF

15-1278 was used in Flow cytometry/Cell sorting to demonstrate a transgenic approach to engineer customised, allergen-specific, human Treg cells, with potential implications for the treatment of allergic diseases.

Sat Jan 01 00:00:00 EST 2011

Uncoupling of proliferation and cytokines from suppression within the CD4+CD25+Foxp3+ T-cell compartment in the 1st year of human type 1 diabetes.

Diabetes

Hughson A,Bromberg I,Johnson B,Quataert S,Jospe N,Fowell DJ

15-1278 was used in Flow cytometry/Cell sorting to determine the relative proportions of effector and regulatory T cells in the CD4+CD25+Foxp3+ T-cell compartment in varying type 1 diabetes disease states.

Mon Aug 01 00:00:00 EDT 2011

A different immunologic profile characterizes patients with HER-2-overexpressing and HER-2-negative locally advanced breast cancer: implications for immune-based therapies.

Breast cancer research : BCR

Muraro E,Martorelli D,Turchet E,Miolo G,Scalone S,Comaro E,Talamini R,Mastorci K,Lombardi D,Perin T,Carbone A,Veronesi A,Crivellari D,Dolcetti R

15-1278 was used in Flow cytometry/Cell sorting to demonstrate that patients with HER2-overexpressing locally advanced breast cancer show more limited tumour-related immune suppression than HER2- patients.

Mon Jun 25 00:00:00 EDT 2012

Efficacious early antiviral activity of HIV Gag- and Pol-specific HLA-B 2705-restricted CD8+ T cells.

Journal of virology

Payne RP,Kløverpris H,Sacha JB,Brumme Z,Brumme C,Buus S,Sims S,Hickling S,Riddell L,Chen F,Luzzi G,Edwards A,Phillips R,Prado JG,Goulder PJ

15-1278 was used in Flow cytometry/Cell sorting to indicate an important role for the B 2705-Gag KK10 response in the control of HIV.

Fri Oct 01 00:00:00 EDT 2010

Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

Published figure using CD127 monoclonal antibody (Product # 15-1278-42) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2009

Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Kim CH

Sat Dec 01 00:00:00 EST 2007

Loss of IL-7 receptor alpha on CD4+ T cells defines terminally differentiated B cell-helping effector T cells in a B cell-rich lymphoid tissue.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Kim CH

Sat Dec 01 00:00:00 EST 2007

生物信息学

蛋白别名: CD127; CD127 antigen; CDw127; IL 7 receptor; IL 7R a; IL 7R subunit alpha; IL 7R α; IL 7Ra; IL 7Rα; IL-7 receptor subunit alpha; IL-7R; IL-7R subunit alpha; IL7 receptor; IL7 receptor subunit alpha; IL7R a; IL7R alpha; IL7R subunit alpha; IL7R α; IL7Rα; interleukin 7 receptor alpha chain; interleukin 7 receptor isoform H5-6; Interleukin-7 receptor subunit alpha; Interleukin7 receptor subunit alpha; MGC107557; sCD127; soluble IL 7 receptor; soluble IL 7R

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基因别名: CD127; CDW127; IL-7R-alpha; IL7R; IL7RA; ILRA

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UniProt ID: (Human) P16871

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Entrez Gene ID: (Human) 3575

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功能

antigen binding interleukin-7 receptor activity protein binding transmembrane signal receptor

参与通路