Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL. This clone has been reported to block mouse APN enzyme activity.
This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01EU/ug.
Rat anti Mouse CD13 antibody, clone R3-63 recognizes mouse aminopeptidase N (APN), a cell surface protein homologous with human CD13.
CD13 (ANPEP) is a 150-170 kDa type II transmembrane zinc-binding ectopeptidase expressed on various cell types. CD13 is a metalloprotease that preferentially catalyzes removal of neutral amino acids from small peptides, thus activating or inactivating bioactive peptides. CD13 also has a role in extracellular matrix degradation, antigen processing and signal transduction, is important in inflammatory responses, regulates intercellular contact, cell motility and vascularization. CD13 is expressed by granulocytes, monocytes, myeloid leukemia cells, and is involved in protection of leukemic cells against apoptosis and its expression associated with poor prognosis of carcinomas. CD13 functions as an aminopeptidase enzyme and is also alpha receptor for coronavirus.
蛋白别名: Alanyl aminopeptidase; Aminopeptidase M; Aminopeptidase N; aminopeptidase N/CD13; AP-M; AP-N; CD13; h AP-N; Membrane protein p161; Microsomal aminopeptidase
基因别名: Anpep; AP-M; AP-N; Apn; Cd13; Lap-1; Lap1; P150
UniProt ID: (Mouse) P97449
Entrez Gene ID: (Mouse) 16790