Invitrogen

CD163 Monoclonal Antibody (GHI/61), PerCP-Cyanine5.5

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CD163 Antibody (MA562218) in Flow

Flow cytometry (surface staining) of CD163 in human peripheral blood. Samples were stained with CD163 monoclonal antibody (Product # MA562218).

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产品信息

MA562218

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

4 µL/100 µL whole blood or 10^6 cells

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

GHI/61

抗原

Hairy cell leukemia cells.

查看免疫原

偶联物

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

激发/发射光谱

489/679 nm 查看光谱

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Size-exclusion chromatography

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

15mM sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。