Invitrogen

CD163 Monoclonal Antibody (eBioGHI/61 (GHI/61)), Biotin, eBioscience™

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CD163 Antibody (13-1639-82) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD11b PE (Product # 12-0118-42) and 0.125 µg of Mouse IgG1 K Isotype Control Biotin (Product # 13-4714-85) (left) or 0.125 µg of Anti-Human CD163 Biotin (right) followed by Streptavidin APC (Product # 17-4314-82). Cells in the monocyte gate were used for analysis.

CD163 Antibody in Immunohistochemistry (IHC)

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产品信息

13-1639-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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流式细胞分析 (Flow)

1 µg/test

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产品规格

种属反应

Human

已发表种属

Human, Non-human primate

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBioGHI/61 (GHI/61)

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_657638

产品详细信息

Description: The eBioGHI/61 monoclonal antibody reacts with human CD163 (Haemoglobin Scavenger Receptor, M130), a member of the macrophage scavenger receptor family. CD163 is expressed by tissue macrophages, circulating blood monocytes, and some reports suggest that it may also be expressed by haematopoietic progenitor cells. CD163 functions as a high affinity scavenger receptor for the complex of haemoglobin and haptoglobin which forms to clear haemoglobin from the blood. The high affinity for CD163 is specific for the haemoglobin-haptoglobin complex, whereas haemoglobin or haptoglobin display no to low affinity toward CD163. Upon ligand binding, CD163 initiates signal transduction which leads to the production of IL-6 and IL-10. Furthermore, it has been demonstrated that cytokines such as IL-6 and IL-10 can induce expression of CD163, whereas proinflammatory stimuli, such as LPS or TNF alpha, can cause shedding of a soluble form of CD163, whose function is unclear.

Applications Reported: This eBioGHI/61 (GHI/61) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioGHI/61 (GHI/61) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

IL-6 facilitates cross-talk between epithelial cells and tumor- associated macrophages in Helicobacter pylori-linked gastric carcinogenesis.

Neoplasia (New York, N.Y.)

Yu B,de Vos D,Guo X,Peng S,Xie W,Peppelenbosch MP,Fu Y,Fuhler GM

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Mon Apr 01 00:00:00 UTC 2024

Germline Neurofibromin 1 mutation enhances the anti-tumour immune response and decreases juvenile myelomonocytic leukaemia tumourigenicity.

British journal of haematology

Wang W,Li X,Qin X,Miao Y,Zhang Y,Li S,Yao R,Yang Y,Yu L,Zhu H,Song L,Mao S,Wang X,Chen J,Feng H,Li Y

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Sat Jul 01 00:00:00 UTC 2023

Inhibition of inflammation and infiltration of M2 macrophages in NSCLC through the ATF3/CSF1 axis: Role of miR-27a-3p.

International journal of experimental pathology

Zhou B,Xu Y,Xu L,Kong Y,Li K,Chen B,Li J

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Fri Dec 01 00:00:00 UTC 2023

SARS-CoV-2 Delta (B.1.617.2) variant replicates and induces syncytia formation in human induced pluripotent stem cell-derived macrophages.

PeerJ

Thaweerattanasinp T,Wanitchang A,Saenboonrueng J,Srisutthisamphan K,Wanasen N,Sungsuwan S,Jongkaewwattana A,Chailangkarn T

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Thu Mar 09 00:00:00 UTC 2023

A positive feedback loop: RAD18-YAP-TGF-β between triple-negative breast cancer and macrophages regulates cancer stemness and progression.

Cell death discovery

Yan X,He Y,Yang S,Zeng T,Hua Y,Bao S,Yang F,Duan N,Sun C,Liang Y,Fu Z,Huang X,Li W,Yin Y

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Tue Apr 12 00:00:00 UTC 2022

M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties.

Cell death discovery

Zhao G,Ding L,Yu H,Wang W,Wang H,Hu Y,Qin L,Deng G,Xie B,Li G,Qi L

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Thu Aug 04 00:00:00 UTC 2022

M1 macrophage-derived exosomes and their key molecule lncRNA HOTTIP suppress head and neck squamous cell carcinoma progression by upregulating the TLR5/NF-κB pathway.

Cell death & disease

Jiang H,Zhou L,Shen N,Ning X,Wu D,Jiang K,Huang X

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Thu Feb 24 00:00:00 UTC 2022

TcpC Inhibits M1 but Promotes M2 Macrophage Polarization via Regulation of the MAPK/NF-κB and Akt/STAT6 Pathways in Urinary Tract Infection.

Cells

Fang J,Ou Q,Wu B,Li S,Wu M,Qiu J,Cen N,Hu K,Che Y,Ma Y,Pan J

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Sun Aug 28 00:00:00 UTC 2022

PTX3 mediates the infiltration, migration, and inflammation-resolving-polarization of macrophages in glioblastoma.

CNS neuroscience & therapeutics

Zhang H,Wang Y,Zhao Y,Liu T,Wang Z,Zhang N,Dai Z,Wu W,Cao H,Feng S,Zhang L,Cheng Q,Liu Z

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Tue Nov 01 00:00:00 UTC 2022

Pulmonary transplantation of alpha-1 antitrypsin (AAT)-transgenic macrophages provides a source of functional human AAT in vivo.

Gene therapy

Janosz E,Hetzel M,Spielmann H,Tumpara S,Rossdam C,Schwabbauer M,Kloos D,von Kaisenberg C,Schambach A,Buettner FFR,Janciauskiene S,Lachmann N,Moritz T

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Wed Sep 01 00:00:00 UTC 2021

Dysregulation of the CD163-Haptoglobin Axis in the Airways of COPD Patients.

Cells

Higham A,Baker JM,Jackson N,Shah R,Lea S,Singh D

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Tue Dec 21 00:00:00 UTC 2021

Macrophages induce malignant traits in mammary epithelium via IKKε/TBK1 kinases and the serine biosynthesis pathway.

EMBO molecular medicine

Wilcz-Villega E,Carter E,Ironside A,Xu R,Mataloni I,Holdsworth J,Jones W,Moreno Béjar R,Uhlik L,Bentham RB,Godinho SA,Dalli J,Grose R,Szabadkai G,Jones L,Hodivala-Dilke K,Bianchi K

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Fri Feb 07 00:00:00 UTC 2020

Extracorporeal Hemadsorption versus Glucocorticoids during Cardiopulmonary Bypass: A Prospective, Randomized, Controlled Trial.

Cardiovascular therapeutics

Taleska Stupica G,Sostaric M,Bozhinovska M,Rupert L,Bosnic Z,Jerin A,Ihan A,Klokocovnik T,Podbregar M

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Mon Jul 06 00:00:00 UTC 2020

Patient iPSC-Derived Macrophages to Study Inborn Errors of the IFN-γ Responsive Pathway.

Cells

Haake K,Neehus AL,Buchegger T,Kühnel MP,Blank P,Philipp F,Oleaga-Quintas C,Schulz A,Grimley M,Goethe R,Jonigk D,Kalinke U,Boisson-Dupuis S,Casanova JL,Bustamante J,Lachmann N

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Wed Feb 19 00:00:00 UTC 2020

CCL5 derived from tumor-associated macrophages promotes prostate cancer stem cells and metastasis via activating β-catenin/STAT3 signaling.

Cell death & disease

Huang R,Wang S,Wang N,Zheng Y,Zhou J,Yang B,Wang X,Zhang J,Guo L,Wang S,Chen Z,Wang Z,Xiang S

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Immunohistochemistry

Thu Apr 16 00:00:00 UTC 2020

Recruitment of monocytes and epigenetic silencing of intratumoral CYP7B1 primarily contribute to the accumulation of 27-hydroxycholesterol in breast cancer.

American journal of cancer research

Shi SZ,Lee EJ,Lin YJ,Chen L,Zheng HY,He XQ,Peng JY,Noonepalle SK,Shull AY,Pei FC,Deng LB,Tian XL,Deng KY,Shi H,Xin HB

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Tue Sep 29 00:00:00 UTC 2020

TIM‑4 blockade of KCs combined with exogenous TGF‑β injection helps to reverse acute rejection and prolong the survival rate of mice receiving liver allografts.

International journal of molecular medicine

Wu H,Xu X,Li J,Gong J,Li M

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Sun Jul 01 00:00:00 UTC 2018

Biochanin a Enhances the Defense Against Salmonella enterica Infection Through AMPK/ULK1/mTOR-Mediated Autophagy and Extracellular Traps and Reversing SPI-1-Dependent Macrophage (MΦ) M2 Polarization.

Frontiers in cellular and infection microbiology

Zhao X,Tang X,Guo N,An Y,Chen X,Shi C,Wang C,Li Y,Li S,Xu H,Liu M,Wang Y,Yu L

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Fri Aug 30 00:00:00 UTC 2019

Widespread Virus Replication in Alveoli Drives Acute Respiratory Distress Syndrome in Aerosolized H5N1 Influenza Infection of Macaques.

Journal of immunology (Baltimore, Md. : 1950)

Wonderlich ER,Swan ZD,Bissel SJ,Hartman AL,Carney JP,O'Malley KJ,Obadan AO,Santos J,Walker R,Sturgeon TJ,Frye LJ,Maiello P,Scanga CA,Bowling JD,Bouwer AL,Duangkhae PA,Wiley CA,Flynn JL,Wang J,Cole KS,Perez DR,Reed DS,Barratt-Boyes SM

13163982 was used in immunohistochemistry to ask if small particle aerosols of virus would penetrate the lower respiratory tract and blanket alveoli where target cells reside

Wed Feb 15 00:00:00 UTC 2017

Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system.

Journal of neurovirology

Garcia-Mesa Y,Jay TR,Checkley MA,Luttge B,Dobrowolski C,Valadkhan S,Landreth GE,Karn J,Alvarez-Carbonell D

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Wed Feb 01 00:00:00 UTC 2017

Alternatively activated macrophages derived from THP-1 cells promote the fibrogenic activities of human dermal fibroblasts.

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society

Zhu Z,Ding J,Ma Z,Iwashina T,Tredget EE

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Mon May 01 00:00:00 UTC 2017

MicroRNA-720 suppresses M2 macrophage polarization by targeting GATA3.

Bioscience reports

Zhong Y,Yi C

Published figure using CD163 monoclonal antibody (Product # 13-1639-82) in Flow Cytometry

Mon Aug 01 00:00:00 UTC 2016

CD169⁺ macrophages provide a niche promoting erythropoiesis under homeostasis and stress.

Nature medicine

Chow A,Huggins M,Ahmed J,Hashimoto D,Lucas D,Kunisaki Y,Pinho S,Leboeuf M,Noizat C,van Rooijen N,Tanaka M,Zhao ZJ,Bergman A,Merad M,Frenette PS

13-1639 was used in Flow cytometry/Cell sorting to elucidate the effect of CD169(+) macrophage depletion on homeostatic and disease-state bone-marrow erythropoiesis.

Mon Apr 01 00:00:00 UTC 2013

Dendritic Cells (DC) Facilitate Detachment of Squamous Carcinoma Cells (SCC), While SCC Promote an Immature CD16(+) DC Phenotype and Control DC Migration.

Cancer microenvironment : official journal of the International Cancer Microenvironment Society

Ramanathapuram LV,Hopkin D,Kurago ZB

13-1639 was used in Flow cytometry/Cell sorting to study the relationship between dendritic cells and squamous carcinoma cells in the tumour microenvironment.