Invitrogen

CD18 Monoclonal Antibody (MEM-48), Alexa Fluor™ 700

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CD18 Antibody (MA562221) in Flow

Flow cytometry (surface staining) of CD18 in human peripheral whole blood. Samples were stained with CD18 monoclonal antibody (Product # MA562221) using a dilution of 4 µL reagent/100 µL of peripheral whole blood. Separation of human lymphocytes (red-filled) from human CD18 negative blood debris (black-dashed).

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产品信息

MA562221

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

4 µL/100 µL whole blood or 10^6 cells

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

MEM-48

抗原

Leukocytes of a patient suffering from a LGL-type leukemia.

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偶联物

Alexa Fluor™ 700 Alexa Fluor™ 700 Alexa Fluor™ 700

激发/发射光谱

696/719 nm 查看光谱

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Size-exclusion chromatography

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

15mM sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

CD18, also known as the integrin beta 2 subunit, is a 90-95 kDa type I transmembrane protein expressed on all leukocytes. It heterodimerizes with the alpha chains of CD11a, CD11b, CD11c, and CD11d to form various leukocyte (beta2) integrins, which are crucial for cellular adhesion and signaling. The CD18/CD11a complex, known as lymphocyte function-associated antigen-1 (LFA-1), plays a significant role in cellular adhesion and inflammatory reactions. CD18 also forms heterodimers with CD11b (Mac-1, CR3), CD11c, and CD11d, contributing to the processes of cell adhesion and cell-surface mediated signaling. These integrins are essential for proper leukocyte migration and mediating intercellular contacts. The absence of CD18 leads to leukocyte adhesion deficiency type I (LAD1), a condition characterized by impaired leukocyte migration. Severe reduction in CD18 expression can result in the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin, which can mediate leukotoxin-induced cytolysis. Defects in the CD18 gene are the cause of LAD1, and two transcript variants encoding the CD18 protein have been identified. The integrin-mediated responses are often a composite of the functions of its individual subunits, highlighting the importance of CD18 in immune function and disease.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CD18; cell surface adhesion glycoprotein (LFA-1/CR3/P150,959 beta subunit precursor); Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; complement component 3 receptor 3 and 4 subunit; complement receptor C3 beta-subunit; Complement receptor C3 subunit beta; integrin beta chain, beta 2; Integrin beta-2; integrin, beta 2 (complement component 3 receptor 3 and 4 subunit); leukocyte cell adhesion molecule CD18; leukocyte-associated antigens CD18/11A, CD18/11B, CD18/11C

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基因别名: CD18; ITGB2; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7

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UniProt ID: (Human) P05107

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Entrez Gene ID: (Human) 3689

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功能

glycoprotein binding protein binding protein kinase binding ICAM-3 receptor activity protein complex binding metal ion binding protein heterodimerization activity cell adhesion molecule binding integrin

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