Invitrogen

CD194 (CCR4) Monoclonal Antibody (D8SEE), Brilliant Violet™ 421, eBioscience™

Name: CD194 (CCR4) Monoclonal Antibody (D8SEE), Brilliant Violet\u2122 421, eBioscience\u2122
Brand: Invitrogen
SKU: 404-1949-42

查看其他19个CCR4抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

CD194 (CCR4) Antibody in Flow Cytometry (Flow)

图: 1 / 1

CD194 (CCR4) Antibody (404-1949-42) in Flow

Normal human peripheral blood cells were stained with CD4 Monoclonal Antibody, FITC (Product # 11-0049-42) and Mouse IgG1 kappa Isotype Control, Brilliant Violet 421 (Product # 404-4714-81) (left) or CD194 (CCR4) Monoclonal Antibody, Brilliant Violet 421 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

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产品信息

404-1949-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.5 µg)/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

D8SEE

偶联物

Brilliant Violet™ 421 Brilliant Violet™ 421 Brilliant Violet™ 421

激发/发射光谱

406/423 nm 查看光谱

形式

liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Description
This D8SEE monoclonal antibody reacts with human CD194 (CCR4). CD194, also known as C-C Chemokine Receptor 4 (CCR4) is a member of the G protein-coupled receptor family, a large family characterized by seven transmembrane alpha helical domains. The ligands for CCR4 are CCL17 (TARC) and CCL22 (MDC), which direct the migration of target cells. CCR4 and its ligands, along with the other receptors CCR10 and CLA, are particularly important for the recruitment of T cells into the skin, where they may also play a role in adhesion and extravasation. Although CCR4 expression was originally believed to be restricted to Th2 cells, it has since been observed in some subsets of Th17, Treg, and Th1 cells. It is not expressed by naive T cells, indicating a role in memory or effector function. Other cells that express CCR4 are activated NK cells, platelets, monocytes, and basophils.

Applications Tested: This D8SEE antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Blocking Buffers
When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) or Brilliant Stain Buffer (Product # 00-4409-75) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information.

Fixation
• Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation.
• Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 407 nm; Emission: 423 nm; Laser: Violet Laser.

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.

靶标信息

CCR4 is a chemokine receptor and is preferentially expressed on type 2 helper T (Th2-type) cells. In contrast to other chemokine receptors, the expression of CCR4 and CCR8 on Th2 cells is transiently increased following TCR and CD28 engagement. Moreover, activated Th1 cells up-regulate CCR4 expression and functional responsiveness to thymus- and activation-regulated chemokine. Chemokines are a group of small (approximately 8 to 14 kD), mostly basic, structurally related molecules that regulate cell trafficking of various types of leukocytes through interactions with a subset of 7-transmembrane, G protein-coupled receptors. Chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Chemokines are divided into 2 major subfamilies, CXC and CC, based on the arrangement of the first 2 of the 4 conserved cysteine residues; the 2 cysteines are separated by a single amino acid in CXC chemokines and are adjacent in CC chemokines.

仅用于科研。不用于诊断过程。未经明确授权不得转售。