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Invitrogen
CD20 Recombinant Rat Monoclonal Antibody (AISB12)
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CD20 Antibody (740152T) in Flow
Flow cytometry analysis of CD20 Recombinant Rat Monoclonal Antibody (AISB12) (Product # 740152T) was performed using Sp2/0 cells. Cells were stained with 0.25 µg of the CD20 Recombinant Rat Monoclonal Antibody (AISB12) (Product # 740152T) (pink histogram) or Rat IgG2a kappa Isotype Control (eBR2a), eBioscience™ (Product # 14-4321-82) (blue histogram), followed by Donkey anti-Rat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 (Product # A78947, 1:1,000). Viable cells were used for analysis, as determined by LIVE/DEAD™ ... View More
产品信息
740152T
产品规格
种属反应
Mouse
宿主/亚型
Rat
/ IgG2a
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
AISB12
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
产品详细信息
Recombinant rat monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
靶标信息
CD20 is a non-glycosylated surface phosphoprotein that has a molecular weight range of 33-37 kDa depending on the degree of phosphorylation. CD20 is expressed on mature and most malignant B cells, in a subpopulation of T lymphocytes and follicular dendritic cells. CD20 expression on B cells is synchronous with the expression of surface IgM and it regulates transmembrane calcium conductance, cell cycle progression and B-cell proliferation. CD20 is also associated with lipid rafts, but the intensity of this association depends on extracellular triggering, employing CD20 conformational change, and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR and CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following monoclonal antibody treatment. Diseases associated with CD20 dysfunction include Ms4a1-related common variable immune deficiency.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: APY; ATOPY; B-cell differentiation antigen Ly-44; B-lymphocyte antigen CD20; CD20; CD20 antigen; Fc epsilon receptor I beta chain; Fc Fragment of IgE high affinity I receptor for beta polypeptide; FCER1B; High affinity immunoglobulin epsilon receptor subunit beta; IgE Fc receptor subunit beta; IGEL; IGER; IGHER; LEU16; Ly44; Lymphocyte antigen 44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 2; MGC3969
基因别名: AA960661; Cd20; Ly-44; Ms4a1; Ms4a2
UniProt ID: (Mouse) P19437
Entrez Gene ID: (Mouse) 12482
