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Invitrogen
CD202b (TIE2) Monoclonal Antibody (TEK4), Functional Grade, eBioscience™
-Functional-Grade-Flow-20170216130641.jpg?time=20220826 "CD202b (TIE2) Antibody in Flow Cytometry (Flow)")
-Functional-Grade-Flow-20170216130641.jpg?time=20220826)
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-Functional-Grade-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD202b (TIE2) Antibody (16-5987-82) in Flow")
.jpg?time=20220826?time=20220826 "CD202b (TIE2) Antibody (16-5987-82) in IHC")
产品信息
16-5987-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
Human,
Mouse
宿主/亚型
Rat
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
TEK4
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
no preservative
保存条件
4° C
运输条件
Ambient (domestic); Wet ice (international)
RRID
AB_469188
产品详细信息
Description: The TEK4 monoclonal antibody reacts with mouse Tie-2, also known as CD202. A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers^-, c-Kit^+, Sca1^+ cells. Long-term multilineage repopulating cells were detected in Tie-2^+, Lineage^-, c-Kit^+, Sca1^+ cells but not in Tie-2^-, Lineage^-, c-Kit^+, Sca1^+ cells.
Applications Reported: The TEK4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The TEK4 antibody has been tested by blocking of staining with fluorochrome conjugated TEK4 on mouse bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
靶标信息
The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells. TEK signaling appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis, and defects in TEK are associated with inherited venous malformations. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Immunoblotting showed that TIE2 expression was increased by thyroid-stimulating hormone and agents that increased intracellular cAMP. HSCs expressing the receptor tyrosine kinase TIE2 are quiescent and antiapoptotic and comprise a side population of HSCs that adhere to osteoblasts in the bone marrow niche.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Angiopoietin-1 receptor; CD202b; CD202b antigen; EC 2.7.10; EC 2.7.10.1; Endothelial tyrosine kinase; HYK; mTIE2; p140 TEK; STK1; TEKT1; Tunica interna endothelial cell kinase; Tyrosine kinase with Ig and EGF homology domains-2; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2
基因别名: AA517024; Cd202b; Hyk; STK1; Tek; Tie-2; Tie2
UniProt ID: (Mouse) Q02858
Entrez Gene ID: (Mouse) 21687
nucleotide binding
protein kinase activity
protein tyrosine kinase activity
transmembrane receptor protein tyrosine kinase activity
receptor activity
protein binding
ATP binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
growth factor binding
transmembrane signal receptor
angiogenesis
patterning of blood vessels
vasculogenesis
positive regulation of protein phosphorylation
endothelial cell proliferation
regulation of endothelial cell proliferation
sprouting angiogenesis
positive regulation of cytokine secretion involved in immune response
protein phosphorylation
cell-matrix adhesion
transmembrane receptor protein tyrosine kinase signaling pathway
heart development
positive regulation of endothelial cell migration
positive regulation of phosphatidylinositol 3-kinase signaling
phosphorylation
single organismal cell-cell adhesion
negative regulation of angiogenesis
peptidyl-tyrosine phosphorylation
hemopoiesis
regulation of cell migration
positive regulation of vascular endothelial growth factor receptor signaling pathway
response to retinoic acid
regulation of establishment or maintenance of cell polarity
positive regulation of peptidyl-serine phosphorylation
substrate adhesion-dependent cell spreading
intracellular signal transduction
positive regulation of protein import into nucleus
negative regulation of apoptotic process
positive regulation of phosphatidylinositol 3-kinase activity
response to estrogen
regulation of angiogenesis
positive regulation of angiogenesis
positive regulation of cell adhesion
protein autophosphorylation
Tie signaling pathway
protein oligomerization
positive regulation of focal adhesion assembly
positive regulation of protein kinase B signaling
heart trabecula formation
positive regulation of ERK1 and ERK2 cascade
regulation of NIK/NF-kappaB signaling
positive regulation of intracellular signal transduction
positive regulation of actin cytoskeleton reorganization
negative regulation of endothelial cell apoptotic process
