Invitrogen

CD24 Monoclonal Antibody (M1/69), Biotin, eBioscience™

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CD24 Antibody (13-0242-82) in Flow

Surface staining of mouse splenocytes with Anti-Mouse CD24 FITC (left), and PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

CD24 Antibody in Immunocytochemistry (ICC/IF)

CD24 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD24 Antibody in Immunohistochemistry (IHC)

CD24 Antibody in T-Cell Activation (TCA)

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产品信息

13-0242-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.125 µg/test

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T细胞活化 (TCA)

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亲和纯化 (AP)

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其他PubMed (Misc)

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产品规格

种属反应

Mouse

已发表种属

Fish, Human, Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

M1/69

偶联物

Biotin Biotin Biotin

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_466397

产品详细信息

Description: The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.

Applications Reported: The M1/69 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The M1/69 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, and control of autoimmunity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence.

Nature communications

Chiang HY,Lu HH,Sudhakar JN,Chen YW,Shih NS,Weng YT,Shui JW

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Tue Feb 15 00:00:00 EST 2022

FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver.

STAR protocols

Braun TW,Kuoch MK,Khandros E,Li H

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Fri Mar 18 00:00:00 EDT 2022

Hormone-Responsive BMP Signaling Expands Myoepithelial Cell Lineages and Prevents Alveolar Precocity in Mammary Gland.

Frontiers in cell and developmental biology

Shao C,Lou P,Liu R,Bi X,Li G,Yang X,Sheng X,Xu J,Lv C,Yu Z

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Tue Aug 03 00:00:00 EDT 2021

Testosterone metabolites differentially regulate obesogenesis and fat distribution.

Molecular metabolism

Sebo ZL,Rodeheffer MS

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Plasmacytoid Dendritic Cells and Type I Interferon Promote Extrafollicular B Cell Responses to Extracellular Self-DNA.

Immunity

Soni C,Perez OA,Voss WN,Pucella JN,Serpas L,Mehl J,Ching KL,Goike J,Georgiou G,Ippolito GC,Sisirak V,Reizis B

13-0242-82 was used in Flow Cytometry to establish extrafollicular B cell differentiation into short-lived AFCs as a key mechanism of anti-DNA autoreactivity and reveal a major contribution of pDCs, endosomal Toll-like receptors (TLRs), and IFN-I to this pathway.

Tue Jun 16 00:00:00 EDT 2020

Membrane expression of the estrogen receptor ERα is required for intercellular communications in the mammary epithelium.

Development (Cambridge, England)

Gagniac L,Rusidzé M,Boudou F,Cagnet S,Adlanmerini M,Jeannot P,Gaide N,Giton F,Besson A,Weyl A,Gourdy P,Raymond-Letron I,Arnal JF,Brisken C,Lenfant F

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Wed Mar 11 00:00:00 EDT 2020

Transcriptomic and proteomic signatures of stemness and differentiation in the colon crypt.

Communications biology

Habowski AN,Flesher JL,Bates JM,Tsai CF,Martin K,Zhao R,Ganesan AK,Edwards RA,Shi T,Wiley HS,Shi Y,Hertel KJ,Waterman ML

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Wed Aug 19 00:00:00 EDT 2020

Analysis of Myeloid Cells in Mouse Tissues with Flow Cytometry.

STAR protocols

Liu Z,Gu Y,Shin A,Zhang S,Ginhoux F

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Fri Jun 19 00:00:00 EDT 2020

An Nfil3-Zeb2-Id2 pathway imposes Irf8 enhancer switching during cDC1 development.

Nature immunology

Bagadia P,Huang X,Liu TT,Durai V,Grajales-Reyes GE,Nitschké M,Modrusan Z,Granja JM,Satpathy AT,Briseño CG,Gargaro M,Iwata A,Kim S,Chang HY,Shaw AS,Murphy TL,Murphy KM

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

Lifelong n-3 Polyunsaturated Fatty Acid Exposure Modulates Size of Mammary Epithelial Cell Populations and Expression of Caveolae Resident Proteins in Fat-1 Mice.

Nutrients

Hillyer LM,Kang JX,Ma DWL

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Tue Oct 15 00:00:00 EDT 2019

Adipocyte hypertrophy and lipid dynamics underlie mammary gland remodeling after lactation.

Nature communications

Zwick RK,Rudolph MC,Shook BA,Holtrup B,Roth E,Lei V,Van Keymeulen A,Seewaldt V,Kwei S,Wysolmerski J,Rodeheffer MS,Horsley V

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Tue Sep 04 00:00:00 EDT 2018

Wnt Effector TCF4 Is Dispensable for Wnt Signaling in Human Cancer Cells.

Genes

Hrckulak D,Janeckova L,Lanikova L,Kriz V,Horazna M,Babosova O,Vojtechova M,Galuskova K,Sloncova E,Korinek V

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases.

Frontiers in immunology

Glal D,Sudhakar JN,Lu HH,Liu MC,Chiang HY,Liu YC,Cheng CF,Shui JW

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Thu Sep 26 00:00:00 EDT 2019

A Paradoxical Tumor-Suppressor Role for the Rac1 Exchange Factor Vav1 in T Cell Acute Lymphoblastic Leukemia.

Cancer cell

Robles-Valero J,Lorenzo-Martín LF,Menacho-Márquez M,Fernández-Pisonero I,Abad A,Camós M,Toribio ML,Espinosa L,Bigas A,Bustelo XR

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Mon Nov 13 00:00:00 EST 2017

Adipocyte Accumulation in the Bone Marrow during Obesity and Aging Impairs Stem Cell-Based Hematopoietic and Bone Regeneration.

Cell stem cell

Ambrosi TH,Scialdone A,Graja A,Gohlke S,Jank AM,Bocian C,Woelk L,Fan H,Logan DW,Schürmann A,Saraiva LR,Schulz TJ

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Metabolic shifts in residual breast cancer drive tumor recurrence.

The Journal of clinical investigation

Havas KM,Milchevskaya V,Radic K,Alladin A,Kafkia E,Garcia M,Stolte J,Klaus B,Rotmensz N,Gibson TJ,Burwinkel B,Schneeweiss A,Pruneri G,Patil KR,Sotillo R,Jechlinger M

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo.

Scientific reports

Ayre DC,Chute IC,Joy AP,Barnett DA,Hogan AM,Grüll MP,Peña-Castillo L,Lang AS,Lewis SM,Christian SL

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in T-Cell Activation

Thu Aug 17 00:00:00 EDT 2017

Skin Adipocyte Stem Cell Self-Renewal Is Regulated by a PDGFA/AKT-Signaling Axis.

Cell stem cell

Rivera-Gonzalez GC,Shook BA,Andrae J,Holtrup B,Bollag K,Betsholtz C,Rodeheffer MS,Horsley V

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Skin Adipocyte Stem Cell Self-Renewal Is Regulated by a PDGFA/AKT-Signaling Axis.

Cell stem cell

Rivera-Gonzalez GC,Shook BA,Andrae J,Holtrup B,Bollag K,Betsholtz C,Rodeheffer MS,Horsley V

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2016

Casitas B-cell lymphoma (Cbl) proteins protect mammary epithelial cells from proteotoxicity of active c-Src accumulation.

Proceedings of the National Academy of Sciences of the United States of America

Mukhopadhyay C,Triplett A,Bargar T,Heckman C,Wagner KU,Naramura M

13-0242 was used in Immunopurification to investigate the protection of MECs from proteotoxic stress.

Tue Dec 20 00:00:00 EST 2016

Casitas B-cell lymphoma (Cbl) proteins protect mammary epithelial cells from proteotoxicity of active c-Src accumulation.

Proceedings of the National Academy of Sciences of the United States of America

Mukhopadhyay C,Triplett A,Bargar T,Heckman C,Wagner KU,Naramura M

13-0242 was used in Immunopurification to investigate the protection of MECs from proteotoxic stress.

Tue Dec 20 00:00:00 EST 2016

Casitas B-cell lymphoma (Cbl) proteins protect mammary epithelial cells from proteotoxicity of active c-Src accumulation.

Proceedings of the National Academy of Sciences of the United States of America

Mukhopadhyay C,Triplett A,Bargar T,Heckman C,Wagner KU,Naramura M

13-0242 was used in Immunopurification to investigate the protection of MECs from proteotoxic stress.

Tue Dec 20 00:00:00 EST 2016

Rapid depot-specific activation of adipocyte precursor cells at the onset of obesity.

Nature cell biology

Jeffery E,Church CD,Holtrup B,Colman L,Rodeheffer MS

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.

Immunity & ageing : I & A

Matsuda T,Yanase S,Takaoka A,Maruyama M

13-0242 was used in Flow cytometry/Cell sorting to show the immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.

Mon Mar 02 00:00:00 EST 2015

Dynamic regulation of CD24 expression and release of CD24-containing microvesicles in immature B cells in response to CD24 engagement.

Immunology

Ayre DC,Elstner M,Smith NC,Moores ES,Hogan AM,Christian SL

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

Dynamic regulation of CD24 expression and release of CD24-containing microvesicles in immature B cells in response to CD24 engagement.

Immunology

Ayre DC,Elstner M,Smith NC,Moores ES,Hogan AM,Christian SL

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

Dynamic regulation of CD24 expression and release of CD24-containing microvesicles in immature B cells in response to CD24 engagement.

Immunology

Ayre DC,Elstner M,Smith NC,Moores ES,Hogan AM,Christian SL

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

A critical role for the regulated wnt-myc pathway in naive T cell survival.

Journal of immunology (Baltimore, Md. : 1950)

Wong C,Chen C,Wu Q,Liu Y,Zheng P

13-0242 was used in Magnetic cell separation to investigate the role of Wnt signalling in mature naive T cells.

Thu Jan 01 00:00:00 EST 2015

Dynamic upregulation of CD24 in pre-adipocytes promotes adipogenesis.

Adipocyte

Smith NC,Fairbridge NA,Pallegar NK,Christian SL

13-0242 was used in Flow cytometry/Cell sorting to investigate the role and regulation of CD24 during adipogenesis, showing that dynamic upregulation of CD24 in pre-adipocytes promotes adipogenesis.

Mon Jul 13 00:00:00 EDT 2015

The Transcriptional Repressor ZNF503/Zeppo2 Promotes Mammary Epithelial Cell Proliferation and Enhances Cell Invasion.

The Journal of biological chemistry

Shahi P,Slorach EM,Wang CY,Chou J,Lu A,Ruderisch A,Werb Z

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Fri Feb 06 00:00:00 EST 2015

Ebf2 is a selective marker of brown and beige adipogenic precursor cells.

Proceedings of the National Academy of Sciences of the United States of America

Wang W,Kissig M,Rajakumari S,Huang L,Lim HW,Won KJ,Seale P

13-0242 was used in Flow cytometry/Cell sorting to study Ebf2 specifically marking and regulating the molecular profile of brown preadipose cells.

Tue Oct 07 00:00:00 EDT 2014

FGF ligands of the postnatal mammary stroma regulate distinct aspects of epithelial morphogenesis.

Development (Cambridge, England)

Zhang X,Martinez D,Koledova Z,Qiao G,Streuli CH,Lu P

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Mon Sep 01 00:00:00 EDT 2014

Rhof promotes murine marginal zone B cell development.

Nagoya journal of medical science

Kishimoto M,Matsuda T,Yanase S,Katsumi A,Suzuki N,Ikejiri M,Takagi A,Ikawa M,Kojima T,Kunishima S,Kiyoi H,Naoe T,Matsushita T,Maruyama M

13-0242 was used in Flow cytometry/Cell sorting to demonstrate that RhoF promotes MZ B cell development in the spleen.

Fri Aug 01 00:00:00 EDT 2014

Rhof promotes murine marginal zone B cell development.

Nagoya journal of medical science

Kishimoto M,Matsuda T,Yanase S,Katsumi A,Suzuki N,Ikejiri M,Takagi A,Ikawa M,Kojima T,Kunishima S,Kiyoi H,Naoe T,Matsushita T,Maruyama M

13-0242 was used in Flow cytometry/Cell sorting to demonstrate that RhoF promotes MZ B cell development in the spleen.

Fri Aug 01 00:00:00 EDT 2014

Prospective identification of functionally distinct stem cells and neurosphere-initiating cells in adult mouse forebrain.

eLife

Mich JK,Signer RA,Nakada D,Pineda A,Burgess RJ,Vue TY,Johnson JE,Morrison SJ

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Wed May 07 00:00:00 EDT 2014

Leukocyte population dynamics and detection of IL-9 as a major cytokine at the mouse fetal-maternal interface.

PloS one

Habbeddine M,Verbeke P,Karaz S,Bobé P,Kanellopoulos-Langevin C

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Wed Jun 10 00:00:00 EDT 2015

Characterization of the adipocyte cellular lineage in vivo.

Nature cell biology

Berry R,Rodeheffer MS

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

Evidence for a multipotent mammary progenitor with pregnancy-specific activity.

Breast cancer research : BCR

Kaanta AS,Virtanen C,Selfors LM,Brugge JS,Neel BG

13-0242 was used in Flow cytometry/Cell sorting to provide data to show the existence of multi-potent mammary progenitor cells that have pregnancy-specific function.

Mon Dec 08 00:00:00 EST 2014

FLT3-ITDs instruct a myeloid differentiation and transformation bias in lymphomyeloid multipotent progenitors.

Cell reports

Mead AJ,Kharazi S,Atkinson D,Macaulay I,Pecquet C,Loughran S,Lutteropp M,Woll P,Chowdhury O,Luc S,Buza-Vidas N,Ferry H,Clark SA,Goardon N,Vyas P,Constantinescu SN,Sitnicka E,Nerlov C,Jacobsen SE

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Thu Jun 27 00:00:00 EDT 2013

Lunatic fringe deficiency cooperates with the Met/Caveolin gene amplicon to induce basal-like breast cancer.

Cancer cell

Xu K,Usary J,Kousis PC,Prat A,Wang DY,Adams JR,Wang W,Loch AJ,Deng T,Zhao W,Cardiff RD,Yoon K,Gaiano N,Ling V,Beyene J,Zacksenhaus E,Gridley T,Leong WL,Guidos CJ,Perou CM,Egan SE

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Tue May 15 00:00:00 EDT 2012

Restricted expression of new guanine nucleotide exchange factor Zizimin2 in aged acquired immune system.

Nagoya journal of medical science

Jia Y,Sakabe I,Matsuda T,Hayakawa T,Maruyama M

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2012

Regulation of TCRβ allelic exclusion by gene segment proximity and accessibility.

Journal of immunology (Baltimore, Md. : 1950)

Kondilis-Mangum HD,Shih HY,Mahowald G,Sleckman BP,Krangel MS

13-0242 was used in Flow cytometry/Cell sorting to analyse the contributions of chromatin accessibility and locus conformation to feedback inhibition.

Thu Dec 15 00:00:00 EST 2011

Stem cell marker prominin-1 regulates branching morphogenesis, but not regenerative capacity, in the mammary gland.

Developmental dynamics : an official publication of the American Association of Anatomists

Anderson LH,Boulanger CA,Smith GH,Carmeliet P,Watson CJ

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2011

A multifunctional 3D co-culture system for studies of mammary tissue morphogenesis and stem cell biology.

PloS one

Campbell JJ,Davidenko N,Caffarel MM,Cameron RE,Watson CJ

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Flow Cytometry

Mon Mar 05 00:00:00 EST 2012

Tumor-initiating function of nucleostemin-enriched mammary tumor cells.

Cancer research

Lin T,Meng L,Li Y,Tsai RY

13-0242 was used in Flow cytometry/Cell sorting to establish features of NS-enriched mammary tumour cells to determine their potential as a therapeutic target.

Mon Nov 15 00:00:00 EST 2010

Robust in vivo transduction of nervous system and neural stem cells by early gestational intra amniotic gene transfer using lentiviral vector.

Molecular therapy : the journal of the American Society of Gene Therapy

Stitelman DH,Endo M,Bora A,Muvarak N,Zoltick PW,Flake AW,Brazelton TR

Published figure using CD24 monoclonal antibody (Product # 13-0242-82) in Immunofluorescence

Wed Sep 01 00:00:00 EDT 2010

Expanded CD23(+)/CD21(hi) B cells in inflamed lymph nodes are associated with the onset of inflammatory-erosive arthritis in TNF-transgenic mice and are targets of anti-CD20 therapy.

Journal of immunology (Baltimore, Md. : 1950)

Li J,Kuzin I,Moshkani S,Proulx ST,Xing L,Skrombolas D,Dunn R,Sanz I,Schwarz EM,Bottaro A

13-0242 was used in Flow cytometry/Cell sorting to investigate the pathogenic role of B lymphocytes in rheumatoid arthritis, showing that CD23(+)/CD21(hi) B cells in inflamed lymph nodes are associated with the onset of inflammatory-erosive arthritis.

Tue Jun 01 00:00:00 EDT 2010

MAP kinase phosphatase activity sets the threshold for thymocyte positive selection.

Proceedings of the National Academy of Sciences of the United States of America

Bettini ML,Kersh GJ

13-0242 was used in Flow cytometry/Cell sorting to demonstrate that MKP activity controls thymocyte cell fate by regulating the TCR signaling threshold that induces positive selection.

Tue Oct 09 00:00:00 EDT 2007

生物信息学

蛋白别名: CD24; cluster of differentiation 24; FLJ22950; FLJ43543; heat stable antigen; HSA; Ly-52; Lymphocyte antigen 52; M1/69-J11D heat stable antigen; MGC75043; Nectadrin; R13-Ag; Signal transducer CD24; X62 heat stable antigen

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基因别名: Cd24; Cd24a; HSA; Ly-52; nectadrin

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UniProt ID: (Mouse) P24807

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Entrez Gene ID: (Mouse) 12484

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功能

protein binding protein kinase binding carbohydrate binding protein tyrosine kinase activator activity

参与通路