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Invitrogen
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
流式细胞分析 (Flow) |
5 µL (0.125 µg)/test | 查看8篇已发表文章 8篇已发表文章 |
| 产品规格 | |
|---|---|
种属反应 |
Human |
已发表种属 |
Human, Mouse |
宿主/亚型 |
Mouse / IgG1, kappa |
推荐的同型对照 |
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™ |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
MIH18 |
偶联物 |
APC
|
激发/发射光谱 |
查看光谱
|
形式 |
Liquid |
浓度 |
5 µL/Test |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.2, with 0.1% gelatin, 0.2% BSA |
内含物 |
0.09% sodium azide |
保存条件 |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_2573238 |
Description: The MIH18 monoclonal antibody reacts with human CD273, also known as PD-L2 or B7-DC, a member of the B7 family that has a predicted molecular weight of approximately 25 kDa, and belongs to the Ig superfamily. B7-DC is primarily expressed by subpopulations of dendritic cells and monocytes/macrophages. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, rather it is a ligand for PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. MIH18 is a useful tool for studies to elucidate the exact function of B7-DC/PD-L2 in APC/T cell interaction, and to characterize the expression pattern of this molecule in humans. MIH18 is a blocking antibody.
Applications Reported: This MIH18 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MIH18 antibody has been pre-titrated and tested by flow cytometric analysis of human monocyte-derived dendritic cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Involved in the costimulatory signal, essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production. [UniProt]
仅用于科研。不用于诊断过程。未经明确授权不得转售。
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
蛋白别名: B7 dendritic cell molecule; B7-DC; Butyrophilin B7-DC; CD273; MGC124039; MGC124040; PD-1 ligand 2; PD-1-ligand 2; PD1 ligand 2; PDCD1 ligand 2; Programmed cell death 1 ligand 2; programmed death ligand 2
基因别名: B7DC; bA574F11.2; Btdc; CD273; PD-L2; PDCD1L2; PDCD1LG2; PDL2
UniProt ID: (Human) Q6JXL9
Entrez Gene ID: (Human) 80380
分子生物学功能:
immunoglobulin receptor superfamily
