FIGURE: 1 / 1
Description: The eBioBT3.1 monoclonal antibody recognizes BT3.1, BT3.2 and BT3.3. The BT family of proteins is a subgroup of the Ig superfamily (IgSF). BT is a glycoprotein that forms a major component of the milk fat globule molecule. In addition to BT, there are six other genes that have been discovered which have been sub-divided into the BT2 (BT2.1, BT2.2, and BT2.3) and BT3 (BT3.1, BT3.2, and BT3.3) families. BT3.1, BT3.2, and BT3.3 share 95% mRNA identity. BT3 molecules are constitutively expressed on the cell surface of antigen-presenting cells, and the IgV-like domain of BT3.1 is sufficient for interaction with its counter-receptor. BT3.1 is expressed by T cells, B cells and CD14+ cells.
Applications Reported: This eBioBT3.1 (20.1, BT3.1) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.
Applications Tested: This eBioBT3.1 (20.1, BT3.1) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene and the BTN2 and BTN3 genes, which have undergone tandem duplication, resulting in 3 copies of each.
Protein Aliases: butyrophilin; Butyrophilin subfamily 3 member A1; butyrophilin, subfamily 3, member A1; CD277; dJ45P21.3 (butyrophilin, subfamily 3, member A1)
Gene Aliases: BT3.1; BTF5; BTN3.1; BTN3A1; CD277
UniProt ID: (Human) O00481
Entrez Gene ID: (Human) 11119
Molecular Function: immunoglobulin receptor superfamily