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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PD-1 (CD279) Antibodies

          Invitrogen

          CD279 (PD-1) Monoclonal Antibody (J43), Super Bright™ 436, eBioscience™

          29 Published Figures
          19 References
          View all (99) PD-1 (CD279) antibodies
          Datasheet
          Protocols
          Tech Support

          Cite CD279 (PD-1) Monoclonal Antibody (J43), Super Bright™ 436, eBioscience™

          Datasheet
          Protocols
          Tech Support
          • Antibody Testing Data (1)
          • Published Figures (29)
          CD279 (PD-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          FIGURE: 1 / 30

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          CD279 (PD-1) Antibody (62-9985-82) in Flow

          Swiss Webster mouse splenocytes were stimulated for 3 days with CD3e and CD28 Monoclonal Antibodies, Functional Grade (Product # 16-0031-82 and Product # 16-0281-82), and then stained with CD4 Monoclonal Antibody, APC (Product # 17-0042-82) and 0.5 µg of Armenian Hamster IgG Isotype Control, Super Bright 436 (Product # 62-4888-82) (left) or 0.5 µg of CD279 Monoclonal Antibody, Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          Product Details

          62-9985-82

          Applications
          Tested Dilution
          Publications

          流式细胞分析 (Flow)

          1.0 µg/test
          查看19篇已发表文章 19篇已发表文章
          产品规格

          Species Reactivity

          Mouse

          Published species

          Not Applicable

          Host/Isotype

          Armenian hamster / IgG

          Recommended Isotype Control

          Armenian Hamster IgG Isotype Control (eBio299Arm), Super Bright™ 436, eBioscience™

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          J43

          Conjugate

          Super Bright™ 436
          • APC 
          • APC-eFluor 780
          • Biotin 
          • Brilliant UV 737
          • Brilliant UV 805
          • eFluor 450
          • eFluor 506
          • FITC
          • Functional Grade
          • PE 
          • PE-Cyanine7
          • PE-eFluor 610
          • PerCP-eFluor 710
          • Super Bright 600
          • Super Bright 645
          • Super Bright 702
          • Super Bright 780
          • Unconjugated

          Excitation/Emission Max

          413/431 nm View spectra spectra

          形式

          Liquid

          浓度

          0.2 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2, with BSA

          内含物

          0.09% sodium azide

          保存条件

          4° C, store in dark, DO NOT FREEZE!

          RRID

          AB_2744826

          Product Specific Information

          Description: The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. It is reported that J43 inhibits the binding of mouse PD-L1-Ig and mouse PD-L2-Ig to PD-1/BHK transfected cells. When administrated in vivo, both intact and Fab of J43 are reported to enhance contact hypersensitivity and exacerbate acute GVHD similar to transfer of PD-1-deficient cells. Injection of J43 also exacerbates EAE and NOD diabetes as do specific antibodies to mouse PD-L1 and PD-L2.

          Applications Reported: This J43 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This J43 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This may be used at less than or equal to 1.0 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

          When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

          Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

          Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

          Target Information

          Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antig en-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. Despite its predicted molecular weight, PD-1 often migrates at higher molecular weight in SDS-PAGE.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          Flow Cytometry
          Panel Builder

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          Bioinformatics

          Protein Aliases: CD279; hPD1; mPD-1; Programmed cell death protein 1; Protein PD-1; Protein PD1; sCD279; soluble CD279

          View more View less

          Gene Aliases: Ly101; PD-1; Pd1; Pdc1; Pdcd1

          View more View less

          UniProt ID: (Mouse) Q02242

          View more View less

          Entrez Gene ID: (Mouse) 18566

          View more View less

          Function(s)
          immune system process negative regulation of tolerance induction apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of T cell apoptotic process
          Process(es)
          protein binding
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

          Learn more
          help_icon

          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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