Invitrogen

CD282 (TLR2) Monoclonal Antibody (6C2), Functional Grade, eBioscience™

CD282 (TLR2) Antibody in Flow Cytometry (Flow)

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CD282 (TLR2) Antibody (16-9021-81) in Flow

Staining of Raw264.7 cells with 0.5 µg Rat IgG2b Isotype Control Functional Grade Purified (Product # 16-4031-81) (open histogram) or 0.5 µg Anti-Mouse CD282 (TLR2) Functional Grade Purified (filled histogram) followed by Anti-Rat IgG Biotin (Product # 13-4813-85) and Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

CD282 (TLR2) Antibody in Immunocytochemistry (ICC/IF)

CD282 (TLR2) Antibody in Immunohistochemistry (IHC)

CD282 (TLR2) Antibody in Western Blot (WB)

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产品信息

16-9021-81

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看1篇已发表文章 1篇已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

1 µg/test

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中和实验 (Neu)

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功能检测 (Functional)

Assay-Dependent

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对照试剂 (Ctrl)

Assay-Dependent

抑制试剂 (IA)

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产品规格

种属反应

Mouse

已发表种属

Human, Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

6C2

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_469265

产品详细信息

Description: The 6C2 monoclonal antibody reacts with mouse Toll-like receptor 2 (TLR2). mouse TLR2 is expressed by the myeloid lineage, including macrophage and dendritic cells in splenocytes and the RAW264.7 cell line. To date, at least twelve members of the Toll family have been identified in human and mouse. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed on the surface of cells and is responsible for distinguishing different pathogens.

Applications Reported: 6C2 has been reported for use in flow cytometric analysis. Unpublished preliminary studies indicate that this antibody may inhibit some aspects of monocyte activation through TLR2, however, it may not result in complete blocking of TLR2 function. For optimal flow cytometry staining with this antibody, it is recommended that a directly conjugated 6C2 (FITC, PE, or Biotin) be used in combination with FcR blocking with anti-mouse CD16/CD32 (clone 93, Product # 14-0161).

Applications Tested: The 6C2 antibody has been tested by flow cytometric analysis of mouse splenocytes or Raw264.7 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Structural transitions modulate the chaperone activities of Grp94.

Proceedings of the National Academy of Sciences of the United States of America

Amankwah YS,Fleifil Y,Unruh E,Collins P,Wang Y,Vitou K,Bates A,Obaseki I,Sugoor M,Alao JP,McCarrick RM,Gewirth DT,Sahu ID,Li Z,Lorigan GA,Kravats AN

Published figure using CD282 (TLR2) monoclonal antibody (Product # 16-9021-81) in Flow Cytometry

Tue Mar 19 00:00:00 UTC 2024

Arf1 Ablation in Colorectal Cancer Cells Activates a Super Signal Complex in DC to Enhance Anti-Tumor Immunity.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Ma H,Fang W,Li Q,Wang Y,Hou SX

Published figure using CD282 (TLR2) monoclonal antibody (Product # 16-9021-81) in Western Blot

Wed Nov 01 00:00:00 UTC 2023

Glial cell line-derived neurotrophic factor increases matrix metallopeptidase 9 and 14 expression in microglia and promotes microglia-mediated glioma progression.

Journal of neuroscience research

Huang Y,Zhang B,Haneke H,Haage V,Lubas M,Yuan Y,Xia P,Motta E,Nanvuma C,Dzaye O,Hu F,Kettenmann H

Published figure using CD282 (TLR2) monoclonal antibody (Product # 16-9021-81) in Flow Cytometry

Thu Apr 01 00:00:00 UTC 2021

Macrophage mediated recognition and clearance of Borrelia burgdorferi elicits MyD88-dependent and -independent phagosomal signals that contribute to phagocytosis and inflammation.

BMC immunology

Benjamin SJ,Hawley KL,Vera-Licona P,La Vake CJ,Cervantes JL,Ruan Y,Radolf JD,Salazar JC

Published figure using CD282 (TLR2) monoclonal antibody (Product # 16-9021-81) in Immunocytochemistry

Mon May 17 00:00:00 UTC 2021

Spatial organization of FcγR and TLR2/1 on phagosome membranes differentially regulates their synergistic and inhibitory receptor crosstalk.

Scientific reports

Li W,Li M,Anthony SM,Yu Y

Published figure using CD282 (TLR2) monoclonal antibody (Product # 16-9021-81) in Immunocytochemistry

Mon Jun 28 00:00:00 UTC 2021

Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis.

Nature communications

Ramakrishna C,Kujawski M,Chu H,Li L,Mazmanian SK,Cantin EM

Published figure using CD282 (TLR2) monoclonal antibody (Product # 16-9021-81) in Flow Cytometry

Tue May 14 00:00:00 UTC 2019

C-type lectin receptor DCIR modulates immunity to tuberculosis by sustaining type I interferon signaling in dendritic cells.

Proceedings of the National Academy of Sciences of the United States of America

Troegeler A,Mercier I,Cougoule C,Pietretti D,Colom A,Duval C,Vu Manh TP,Capilla F,Poincloux R,Pingris K,Nigou J,Rademann J,Dalod M,Verreck FA,Al Saati T,Lugo-Villarino G,Lepenies B,Hudrisier D,Neyrolles O

16-9021 was used in Flow cytometry/Cell sorting to reveal the modulatory role of the C-type lectin, DCIR, in tuberculosis-associated inflammation.

Tue Jan 24 00:00:00 UTC 2017

Upregulation of Intestinal Barrier Function in Mice with DSS-Induced Colitis by a Defined Bacterial Consortium Is Associated with Expansion of IL-17A Producing Gamma Delta T Cells.

Frontiers in immunology

Li M,Wang B,Sun X,Tang Y,Wei X,Ge B,Tang Y,Deng Y,He C,Yuan J,Li X

Published figure using CD282 (TLR2) monoclonal antibody (Product # 16-9021-81) in Flow Cytometry

Wed Nov 20 00:00:00 UTC 2019

Dectin-2 is a primary receptor for NLRP3 inflammasome activation in dendritic cell response to Histoplasma capsulatum.

PLoS pathogens

Chang TH,Huang JH,Lin HC,Chen WY,Lee YH,Hsu LC,Netea MG,Ting JP,Wu-Hsieh BA

16-9021 was used in Blocking experiments to investigate how H. capsulatum induces inflammasome activation and IL-1β production, showing that dectin-2 is a primary receptor for NLRP3 inflammasome activation.

Sat Jul 01 00:00:00 UTC 2017

Immunology

Xu F,Zhang C,Zou Z,Fan EKY,Chen L,Li Y,Billiar TR,Wilson MA,Shi X,Fan J

Published figure using CD282 (TLR2) monoclonal antibody (Product # 16-9021-81) in Flow Cytometry

Tue Aug 01 00:00:00 UTC 2017

A polysaccharide component from Strongylocentrotus nudus eggs inhibited hepatocellular carcinoma in mice by activating T lymphocytes.

Oncology letters

Zhang M,Liu Y,Li J,Ke M,Yu J,Dou J,Wang H,Zhou C

16-9021 was used in Functional assays to indicate that SEP-S is a polysaccharide component possessing high anti-hepatocellular carcinoma activity.

Wed Mar 01 00:00:00 UTC 2017

Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal Gamma-Delta T Cells.

Frontiers in pharmacology

Li M,Gao J,Tang Y,Liu M,Wu S,Qu K,Long X,Li H,Liu M,Liu Y,Yuan J,Mao L,Liu Y,Zheng X,Wang E,Wang J,Yang Y

Published figure using CD282 (TLR2) monoclonal antibody (Product # 16-9021-81) in Flow Cytometry

Wed Sep 30 00:00:00 UTC 2020

Dectin-1 Regulates Hepatic Fibrosis and Hepatocarcinogenesis by Suppressing TLR4 Signaling Pathways.

Cell reports

Seifert L,Deutsch M,Alothman S,Alqunaibit D,Werba G,Pansari M,Pergamo M,Ochi A,Torres-Hernandez A,Levie E,Tippens D,Greco SH,Tiwari S,Ly NNG,Eisenthal A,van Heerden E,Avanzi A,Barilla R,Zambirinis CP,Rendon M,Daley D,Pachter HL,Hajdu C,Miller G

16-9021 was used in Flow cytometry/Cell sorting to determine a link between Dectin-1 and the regulation of sterile inflammation and oncogenesis.

Tue Dec 01 00:00:00 UTC 2015

Dectin-1 Regulates Hepatic Fibrosis and Hepatocarcinogenesis by Suppressing TLR4 Signaling Pathways.

Cell reports

16-9021 was used in Flow cytometry/Cell sorting to determine a link between Dectin-1 and the regulation of sterile inflammation and oncogenesis.

Tue Dec 01 00:00:00 UTC 2015

Priming of Toll-like receptor 4 pathway in mesenchymal stem cells increases expression of B cell activating factor.

Biochemical and biophysical research communications

Yan H,Wu M,Yuan Y,Wang ZZ,Jiang H,Chen T

16-9021 was used in Blocking experiments to suggest that TLR4 and downstream pathways in MSCs exert an important function in B lymphocyte-related immune regulation.

Fri May 30 00:00:00 UTC 2014

Priming of Toll-like receptor 4 pathway in mesenchymal stem cells increases expression of B cell activating factor.

Biochemical and biophysical research communications

Yan H,Wu M,Yuan Y,Wang ZZ,Jiang H,Chen T

16-9021 was used in Blocking experiments to suggest that TLR4 and downstream pathways in MSCs exert an important function in B lymphocyte-related immune regulation.

Fri May 30 00:00:00 UTC 2014

Priming of Toll-like receptor 4 pathway in mesenchymal stem cells increases expression of B cell activating factor.

Biochemical and biophysical research communications

Yan H,Wu M,Yuan Y,Wang ZZ,Jiang H,Chen T

16-9021 was used in Blocking experiments to suggest that TLR4 and downstream pathways in MSCs exert an important function in B lymphocyte-related immune regulation.

Fri May 30 00:00:00 UTC 2014

Protective role for TLR4 signaling in atherosclerosis progression as revealed by infection with a common oral pathogen.

Journal of immunology (Baltimore, Md. : 1950)

Hayashi C,Papadopoulos G,Gudino CV,Weinberg EO,Barth KR,Madrigal AG,Chen Y,Ning H,LaValley M,Gibson FC,Hamilton JA,Genco CA

Published figure using CD282 (TLR2) monoclonal antibody (Product # 16-9021-81) in Immunohistochemistry

Mon Oct 01 00:00:00 UTC 2012

Persistence versus escape: Aspergillus terreus and Aspergillus fumigatus employ different strategies during interactions with macrophages.

PloS one

Slesiona S,Gressler M,Mihlan M,Zaehle C,Schaller M,Barz D,Hube B,Jacobsen ID,Brock M

16-9021 was used in Blocking experiments to show that A. terreus and A. fumigatus have evolved significantly different host immune evasion strategies.

Mon Sep 24 00:00:00 UTC 2012

Plasmodium yoelii blood-stage primes macrophage-mediated innate immune response through modulation of toll-like receptor signalling.

Malaria journal

Fu Y,Ding Y,Zhou T,Fu X,Xu W

16-9021 was used in Flow cytometry/Cell sorting to study the immune response following infection of a lethal or non-lethal strain of rodent malaria parasites.

Sun Apr 01 00:00:00 UTC 2012

TCA cycle inactivation in Staphylococcus aureus alters nitric oxide production in RAW 264.7 cells.

Molecular and cellular biochemistry

Massilamany C,Gangaplara A,Gardner DJ,Musser JM,Steffen D,Somerville GA,Reddy J

16-9021 was used in Flow cytometry/Cell sorting to study the mechanisms by which Staphylococci can evade innate immune responses and enhance their ability to survive in infected hosts.

Thu Sep 01 00:00:00 UTC 2011

Production of autoantibodies by murine B-1a cells stimulated with Helicobacter pylori urease through toll-like receptor 2 signaling.

Infection and immunity

Kobayashi F,Watanabe E,Nakagawa Y,Yamanishi S,Norose Y,Fukunaga Y,Takahashi H

16-9021 was used in Flow cytometry/Cell sorting to determine the role of functional urease expression in the production of various autoantibodies and the induction of autoimmune disorders.

Thu Dec 01 00:00:00 UTC 2011

Distinct roles of complement receptor 3, Dectin-1, and sialic acids in murine macrophage interaction with Histoplasma yeast.

Journal of leukocyte biology

Lin JS,Huang JH,Hung LY,Wu SY,Wu-Hsieh BA

16-9021 was used in Blocking experiments to delineate the distinct roles of CR3, Dectin-1, and sialic acids in the interaction with Histoplasma.

Thu Jul 01 00:00:00 UTC 2010

IL-33 exacerbates eosinophil-mediated airway inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Stolarski B,Kurowska-Stolarska M,Kewin P,Xu D,Liew FY

16-9021 was used in Flow cytometry/Cell sorting to demonstrate that the IL-33/ST2 signaling pathway activates airway eosinophils that exacerbate airway inflammation in an autocrine and paracrine manner.