Invitrogen

CD284 (TLR4) Monoclonal Antibody (HTA125), Alexa Fluor™ 488, eBioscience™

CD284 (TLR4) Antibody in Flow Cytometry (Flow)

图: 1 / 37

CD284 (TLR4) Antibody (53-9917-42) in Flow

Staining of human TLR4/MD2-transfected cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD284 (TLR4) Alexa Fluor® 488 (filled histogram). Total viable cells were used for analysis.

CD284 (TLR4) Antibody in Immunocytochemistry (ICC/IF)

CD284 (TLR4) Antibody in Immunohistochemistry (IHC)

CD284 (TLR4) Antibody in Western Blot (WB)

CD284 (TLR4) Antibody in Western Blot, Neutralization (WB, Neu)

CD284 (TLR4) Antibody in Western Blot, Flow Cytometry (WB, Flow)

CD284 (TLR4) Antibody in Neutralization (Neu)

Product Image Gallery

产品信息

53-9917-42

应用

建议稀释比

已发表文章

免疫印迹 (WB)

查看4篇已发表文章 4篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（石蜡） (IHC (P))

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

查看3篇已发表文章 3篇已发表文章

流式细胞分析 (Flow)

5 µL (1 µg)/test

查看22篇已发表文章 22篇已发表文章

酶联免疫吸附实验 (ELISA)

查看1篇已发表文章 1篇已发表文章

中和实验 (Neu)

查看8篇已发表文章 8篇已发表文章

功能检测 (Functional)

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看1篇已发表文章 1篇已发表文章

产品规格

种属反应

Human

已发表种属

Human, Shark

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

HTA125

偶联物

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

激发/发射光谱

499/520 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_1963634

产品详细信息

Description: The HTA125 monoclonal antibody reacts with human Toll-like receptor 4 (TLR4). So far, at least ten members of the Toll family have been identified in humans. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with another molecule called MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. TLR4 is expressed by peripheral blood monocytes. HTA125 has been reported to immunoprecipitate human TLR4 (~100 kDa) from transfected cells. Most TLR cell surface expression, especially TLR1 and TLR4, occurs at low levels on monocytes and at even lower levels on other cell types including granulocytes and immature dendritic cells (iDC). Furthermore, a relatively high degree of variability in TLR surface expression has been reported among normal donors.

It is highly recommended that for optimal staining of TLR4, whole blood be stained using the lysed whole blood protocol (found in BestProtocols®) rather than Ficoll-gradient prepared normal human peripheral blood cells. The use of a density gradient appears to reduce the staining intensity significantly.

Applications Reported:This HTA125 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HTA125 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Furthermore brighter fluorophores such as the APC and PE are better choices for looking at endogenous levels of TLR4.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Mast cell tolerance in the skin microenvironment to commensal bacteria is controlled by fibroblasts.

Cell reports

Di Nardo A,Chang YL,Alimohammadi S,Masuda-Kuroki K,Wang Z,Sriram K,Insel PA

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Tue May 30 00:00:00 UTC 2023

Saharan dust induces NLRP3-dependent inflammatory cytokines in an alveolar air-liquid interface co-culture model.

Particle and fibre toxicology

Bredeck G,Dobner J,Stahlmecke B,Fomba KW,Herrmann H,Rossi A,Schins RPF

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Fri Oct 20 00:00:00 UTC 2023

Mechanical Compression by Simulating Orthodontic Tooth Movement in an In Vitro Model Modulates Phosphorylation of AKT and MAPKs via TLR4 in Human Periodontal Ligament Cells.

International journal of molecular sciences

Roth CE,Craveiro RB,Niederau C,Malyaran H,Neuss S,Jankowski J,Wolf M

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Neutralization

Fri Jul 22 00:00:00 UTC 2022

Lys694Arg polymorphism leads to blunted responses to LPS by interfering TLR4 with recruitment of MyD88.

Innate immunity

Yang Y,Hu Y,Zhou Y,Liang T,Tang H,Ju H,Shi Q,Fang H

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Western Blot

Sun Aug 01 00:00:00 UTC 2021

Inflammatory signaling in dengue-infected platelets requires translation and secretion of nonstructural protein 1.

Blood advances

Quirino-Teixeira AC,Rozini SV,Barbosa-Lima G,Coelho DR,Carneiro PH,Mohana-Borges R,Bozza PT,Hottz ED

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Neutralization

Tue May 12 00:00:00 UTC 2020

Grindelia squarrosa Extract and Grindelic Acid Modulate Pro-inflammatory Functions of Respiratory Epithelium and Human Macrophages.

Frontiers in pharmacology

Gierlikowska B,Filipek A,Gierlikowski W,Kania D,Stefańska J,Demkow U,Kiss AK

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Sat Feb 06 00:00:00 UTC 2021

Bacterial lipopolysaccharide and antimicrobial LL-37 enhance ICAM-1 expression and NF-κB p65 phosphorylation in senescent endothelial cells.

International journal of molecular medicine

Suzuki K,Ohkuma M,Nagaoka I

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Tue Oct 01 00:00:00 UTC 2019

Dietary Toll-Like Receptor Stimulants Promote Hepatic Inflammation and Impair Reverse Cholesterol Transport in Mice via Macrophage-Dependent Interleukin-1 Production.

Frontiers in immunology

Faraj TA,Stover C,Erridge C

53-9917-42 was used in Flow Cytometry to demonstrate the link between consumption of processed food and the cardiovascular risks and how this can be combated by food microbiota.

Mon Oct 19 00:00:00 UTC 2020

Toll‑Like receptor 4 promotes the phosphorylation of CRMP2 via the activation of Rho‑kinase in MCAO rats.

Molecular medicine reports

Li XB,Ding MX,Ding CL,Li LL,Feng J,Yu XJ

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Neutralization

Sun Jul 01 00:00:00 UTC 2018

n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils.

Molecular medicine reports

Wu B,Wang L,Jiang L,Dong L,Xu F,Lu Y,Jin J,Wang Z,Liang G,Shan X

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Mon Jan 01 00:00:00 UTC 2018

Butyrate upregulates the TLR4 expression and the phosphorylation of MAPKs and NK-κB in colon cancer cell in vitro.

Oncology letters

Xiao T,Wu S,Yan C,Zhao C,Jin H,Yan N,Xu J,Wu Y,Li C,Shao Q,Xia S

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Mon Oct 01 00:00:00 UTC 2018

Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function.

Scientific reports

Hamon R,Tran HB,Roscioli E,Ween M,Jersmann H,Hodge S

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Fri Sep 07 00:00:00 UTC 2018

Altered Toll-like receptor expression and function in HPV-associated oropharyngeal carcinoma.

Oncotarget

Tobouti PL,Bolt R,Radhakrishnan R,de Sousa SCOM,Hunter KD

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Tue Jan 02 00:00:00 UTC 2018

A low dose lipid infusion is sufficient to induce insulin resistance and a pro-inflammatory response in human subjects.

PloS one

Liang H,Lum H,Alvarez A,Garduno-Garcia JJ,Daniel BJ,Musi N

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Fri Jul 27 00:00:00 UTC 2018

HMGB1 is upregulated in the airways in asthma and potentiates airway smooth muscle contraction via TLR4.

The Journal of allergy and clinical immunology

Di Candia L,Gomez E,Venereau E,Chachi L,Kaur D,Bianchi ME,Challiss RAJ,Brightling CE,Saunders RM

53-9917 was used in Flow cytometry/Cell sorting to investigate the redox state of airway high-mobility group box 1 (HMGB1) and its receptors in airway inflammation including asthma.

Tue Aug 01 00:00:00 UTC 2017

Platelet proteome reveals novel pathways of platelet activation and platelet-mediated immunoregulation in dengue.

PLoS pathogens

Trugilho MRO,Hottz ED,Brunoro GVF,Teixeira-Ferreira A,Carvalho PC,Salazar GA,Zimmerman GA,Bozza FA,Bozza PT,Perales J

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Neutralization

Mon May 01 00:00:00 UTC 2017

Early detection of neonatal group B streptococcus sepsis and the possible diagnostic utility of IL-6, IL-8, and CD11b in a human umbilical cord blood in vitro model.

Infection and drug resistance

Nakstad B,Sonerud T,Solevåg AL

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Fri Jul 29 00:00:00 UTC 2016

Picfeltarraenin IA inhibits lipopolysaccharide-induced inflammatory cytokine production by the nuclear factor-κB pathway in human pulmonary epithelial A549 cells.

Oncology letters

Shi R,Wang Q,Ouyang Y,Wang Q,Xiong X

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Neutralization

Mon Feb 01 00:00:00 UTC 2016

Comparative miRNA-Based Fingerprinting Reveals Biological Differences in Human Olfactory Mucosa- and Bone-Marrow-Derived Mesenchymal Stromal Cells.

Stem cell reports

Lindsay SL,Johnstone SA,McGrath MA,Mallinson D,Barnett SC

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Tue May 10 00:00:00 UTC 2016

Comparative miRNA-Based Fingerprinting Reveals Biological Differences in Human Olfactory Mucosa- and Bone-Marrow-Derived Mesenchymal Stromal Cells.

Stem cell reports

Lindsay SL,Johnstone SA,McGrath MA,Mallinson D,Barnett SC

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Tue May 10 00:00:00 UTC 2016

Lipopolysaccharide induces SBD-1 expression via the P38 MAPK signaling pathway in ovine oviduct epithelial cells.

Lipids in health and disease

Li Q,Bao F,Zhi D,Liu M,Yan Q,Zheng X,Ren L,Cong S,Li Y,Cao G

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Neutralization

Thu Aug 11 00:00:00 UTC 2016

Lipopolysaccharide induces SBD-1 expression via the P38 MAPK signaling pathway in ovine oviduct epithelial cells.

Lipids in health and disease

Li Q,Bao F,Zhi D,Liu M,Yan Q,Zheng X,Ren L,Cong S,Li Y,Cao G

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Neutralization

Thu Aug 11 00:00:00 UTC 2016

Human coagulation factor X-adenovirus type 5 complexes poorly stimulate an innate immune response in human mononuclear phagocytes.

Journal of virology

Eichholz K,Mennechet FJ,Kremer EJ

53-9917-42 was used in Immunocytochemistry to determine whether human FX-armoured HAd type 5 was capable of activating innate immune sensors in primary human mononuclear phagocytes.

Sun Mar 01 00:00:00 UTC 2015

TLR4 Expression Is Associated with Left Ventricular Dysfunction in Patients Undergoing Coronary Artery Bypass Surgery.

PloS one

Avlas O,Bragg A,Fuks A,Nicholson JD,Farkash A,Porat E,Aravot D,Levy-Drummer RS,Cohen C,Shainberg A,Arad M,Hochhauser E

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Fri Mar 04 00:00:00 UTC 2016

TLR4 Expression Is Associated with Left Ventricular Dysfunction in Patients Undergoing Coronary Artery Bypass Surgery.

PloS one

Avlas O,Bragg A,Fuks A,Nicholson JD,Farkash A,Porat E,Aravot D,Levy-Drummer RS,Cohen C,Shainberg A,Arad M,Hochhauser E

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Fri Mar 04 00:00:00 UTC 2016

Innate Response Activator (IRA) B Cells Reside in Human Tonsils and Internalize Bacteria In Vitro.

PloS one

Chiappini N,Cantisani R,Pancotto L,Ruggiero P,Rosa D,Manetti A,Romano A,Montagnani F,Bertholet S,Castellino F,Del Giudice G

53-9917 was used in Flow cytometry/Cell sorting to study the role for hIRA B cells in the effector immune response to infections in tonsils.

Tue Apr 19 00:00:00 UTC 2016

The COP II adaptor protein TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane.

Science signaling

Liaunardy-Jopeace A,Bryant CE,Gay NJ

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Tue Jul 29 00:00:00 UTC 2014

The COP II adaptor protein TMED7 is required to initiate and mediate the delivery of TLR4 to the plasma membrane.

Science signaling

Liaunardy-Jopeace A,Bryant CE,Gay NJ

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Tue Jul 29 00:00:00 UTC 2014

Biologically active polymers from spontaneous carotenoid oxidation: a new frontier in carotenoid activity.

PloS one

Johnston JB,Nickerson JG,Daroszewski J,Mogg TJ,Burton GW

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Immunocytochemistry

Thu Jun 25 00:00:00 UTC 2015

E-cadherin interactions are required for Langerhans cell differentiation.

European journal of immunology

Mayumi N,Watanabe E,Norose Y,Watari E,Kawana S,Geijtenbeek TB,Takahashi H

53-9917 was used in Western Blotting to show that E-cadherin expressed by epidermal keratinocytes is crucial for differentiation of LCs.

Tue Jan 01 00:00:00 UTC 2013

E-cadherin interactions are required for Langerhans cell differentiation.

European journal of immunology

Mayumi N,Watanabe E,Norose Y,Watari E,Kawana S,Geijtenbeek TB,Takahashi H

53-9917 was used in Western Blotting to show that E-cadherin expressed by epidermal keratinocytes is crucial for differentiation of LCs.

Tue Jan 01 00:00:00 UTC 2013

E-cadherin interactions are required for Langerhans cell differentiation.

European journal of immunology

Mayumi N,Watanabe E,Norose Y,Watari E,Kawana S,Geijtenbeek TB,Takahashi H

53-9917 was used in Western Blotting to show that E-cadherin expressed by epidermal keratinocytes is crucial for differentiation of LCs.

Tue Jan 01 00:00:00 UTC 2013

Body fluid exosomes promote secretion of inflammatory cytokines in monocytic cells via Toll-like receptor signaling.

The Journal of biological chemistry

Bretz NP,Ridinger J,Rupp AK,Rimbach K,Keller S,Rupp C,Marmé F,Umansky L,Umansky V,Eigenbrod T,Sammar M,Altevogt P

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Fri Dec 20 00:00:00 UTC 2013

Body fluid exosomes promote secretion of inflammatory cytokines in monocytic cells via Toll-like receptor signaling.

The Journal of biological chemistry

Bretz NP,Ridinger J,Rupp AK,Rimbach K,Keller S,Rupp C,Marmé F,Umansky L,Umansky V,Eigenbrod T,Sammar M,Altevogt P

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Fri Dec 20 00:00:00 UTC 2013

Iron sucrose impairs phagocytic function and promotes apoptosis in polymorphonuclear leukocytes.

American journal of nephrology

Ichii H,Masuda Y,Hassanzadeh T,Saffarian M,Gollapudi S,Vaziri ND

53-9917 was used in Flow cytometry/Cell sorting to elucidate the mechanisms and effects of the use of IV iron preparations against bacteria.

Thu Nov 29 00:00:00 UTC 2012

TLR4 protein contributes to cigarette smoke-induced matrix metalloproteinase-1 (MMP-1) expression in chronic obstructive pulmonary disease.

The Journal of biological chemistry

Geraghty P,Dabo AJ,D'Armiento J

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Immunofluorescence

Fri Aug 26 00:00:00 UTC 2011

TLR4 protein contributes to cigarette smoke-induced matrix metalloproteinase-1 (MMP-1) expression in chronic obstructive pulmonary disease.

The Journal of biological chemistry

Geraghty P,Dabo AJ,D'Armiento J

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Immunofluorescence

Fri Aug 26 00:00:00 UTC 2011

Triggering of Toll-like receptor 4 expressed on human head and neck squamous cell carcinoma promotes tumor development and protects the tumor from immune attack.

Cancer research

Szczepanski MJ,Czystowska M,Szajnik M,Harasymczuk M,Boyiadzis M,Kruk-Zagajewska A,Szyfter W,Zeromski J,Whiteside TL

53-9917 was used in Flow cytometry/Cell sorting to identify that triggering of TLR4 protects tumour cells from NK-92 cell mediated lysis.

Wed Apr 01 00:00:00 UTC 2009

Candesartan inhibits Toll-like receptor expression and activity both in vitro and in vivo.

Atherosclerosis

Dasu MR,Riosvelasco AC,Jialal I

Published figure using CD284 (TLR4) monoclonal antibody (Product # 53-9917-42) in Flow Cytometry

Thu Jan 01 00:00:00 UTC 2009

Targeting of DC-SIGN on human dendritic cells by minor fimbriated Porphyromonas gingivalis strains elicits a distinct effector T cell response.

Journal of immunology (Baltimore, Md. : 1950)

Zeituni AE,Jotwani R,Carrion J,Cutler CW

53-9917 was used in Flow cytometry/Cell sorting to investigate the DC receptors involved in the uptake of P. gingivalis and the induced effector immune response.