Invitrogen

CD29 (Integrin beta 1) Monoclonal Antibody (eBioHMb1-1 (HMb1-1)), APC-eFluor™ 780, eBioscience™

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产品信息

47-0291-82

应用
建议稀释比
已发表文章

流式细胞分析 (Flow)

0.5 µg/test
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产品规格

种属反应

Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Armenian hamster / IgG

推荐的同型对照

Armenian Hamster IgG Isotype Control (eBio299Arm), APC-eFluor™ 780, eBioscience™

分类

Monoclonal

类型

Antibody

克隆号

eBioHMb1-1 (HMb1-1)

偶联物

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

激发/发射光谱

756/785 nm 查看光谱 spectra

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_11218499

产品详细信息

Description: The eBioHMb1-1 monoclonal antibody reacts with mouse and rat CD29 (integrin beta 1), a 110-120 kDa member of the beta integrin family expressed by leukocytes, endothelial, smooth muscle and epithelial cells. CD29 binds non-covalently with the alpha integrins CD49a-f to form the VLA-1 through VLA-6 complexes, as well as with CD51. These alpha-beta integrin heterodimers are capable of mediating a variety of cellular responses including adhesion, trafficking, proliferaton and differentiation. All integrins which include CD29 bind to extracellular matrix proteins including collagen, laminin, fibronectin and vitronectin, whereas some CD29-containing integrins can also interact with cellular receptors such as VCAM-1 and MadCAM-1.

Applications Reported: This eBioHMb1-1 (HMb1-1) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioHMb1-1 (HMb1-1) antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD29, also known as ITGB1 or Integrin Subunit Beta 1, is a 110 kDa cell surface glycoprotein widely expressed by various cells, including all leukocytes. It forms non-covalently linked heterodimers with at least six different alpha integrins (CD49a-f), creating VLA-1 through VLA-6 complexes, as well as with CD51. These alpha-beta integrin heterodimers mediate a range of cellular responses, including adhesion, trafficking, proliferation, and differentiation. CD29-containing integrins bind to extracellular matrix proteins such as collagen, laminin, fibronectin, and vitronectin. Some integrins also interact with cellular receptors like VCAM-1 and MadCAM-1. CD29 plays a crucial role in immune cell trafficking from blood to tissue, particularly during leukocyte emigration and extravascular migration. It is involved in regulating tissue damage and disease symptoms related to inflammatory bowel disease. In the presence of anti-tumor drugs, decreased expression of ITGB1 correlates with multidrug resistance in tumor cells. ITGB1-dependent mechanisms enhance cytokine secretion in human airway smooth muscle in response to IL-1beta, facilitated by fibronectin and type I collagen. More than eight beta subunits with numerous splice variant isoforms have been identified in mammals, including two major forms: beta1A and beta1D. Beta1A is present in all tissues except cardiac and skeletal muscle, which express the beta1D variant.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: Beta OL; Beta oligodendroglia; CD29; Fibronectin receptor subunit beta; integrin beta 1 (fibronectin receptor beta); Integrin beta-1; Integrin beta1; integrin VLA-4 subunit beta; ITBG1D; VLA-4 subunit beta

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基因别名: 4633401G24Rik; AA409975; AA960159; CD29; ENSMUSG00000051907; Fnrb; Gm9863; gpIIa; Itgb1

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UniProt ID: (Mouse) P09055, (Rat) P49134

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Entrez Gene ID: (Mouse) 16412, (Rat) 24511

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功能
glycoprotein binding fibronectin binding protease binding actin binding receptor activity receptor binding integrin binding protein binding collagen binding kinase binding protein kinase binding protein domain specific binding protein complex binding peptide binding laminin binding metal ion binding protein heterodimerization activity cell adhesion molecule binding alpha-actinin binding collagen binding involved in cell-matrix adhesion integrin
参与通路
G1/S transition of mitotic cell cycle in utero embryonic development cell fate specification tissue homeostasis cell migration involved in sprouting angiogenesis cellular calcium ion homeostasis cell adhesion leukocyte cell-cell adhesion cell-matrix adhesion calcium-independent cell-matrix adhesion transforming growth factor beta receptor signaling pathway integrin-mediated signaling pathway regulation of G-protein coupled receptor protein signaling pathway positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration visual learning regulation of collagen catabolic process positive regulation of cell-substrate adhesion positive regulation of neuron projection development cell migration formation of radial glial scaffolds positive regulation of cell migration neuron projection development negative regulation of cell projection organization cell-substrate adhesion protein transport within lipid bilayer heterotypic cell-cell adhesion negative regulation of Rho protein signal transduction positive regulation of apoptotic process stress fiber assembly positive regulation of MAPK cascade sarcomere organization negative regulation of cell differentiation negative regulation of neuron differentiation positive regulation of neuron differentiation positive regulation of endocytosis axon extension cardiac muscle tissue development dendrite morphogenesis positive regulation of peptidyl-tyrosine phosphorylation leukocyte tethering or rolling regulation of cell cycle cardiac muscle cell differentiation bicellular tight junction assembly positive regulation of establishment of protein localization to plasma membrane negative regulation of anoikis response to activity response to nutrient levels cell-cell adhesion mediated by integrin response to gonadotropin response to drug mesodermal cell differentiation maternal process involved in female pregnancy cellular response to mechanical stimulus cellular response to vitamin D cellular response to ionizing radiation response to transforming growth factor beta
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