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Invitrogen
CD3 Monoclonal Antibody (1F4)
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产品信息
MA1-80469
应用
建议稀释比
已发表文章
产品规格
种属反应
Rat
已发表种属
Human
宿主/亚型
Mouse
/ IgM
分类
Monoclonal
类型
Antibody
克隆号
1F4
抗原
F344 rat T cells stimulated with PMA (TPA) and calcium ionophore.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Ammonium sulfate precipitation
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This clone is suitable for use on paraffin embedded material using an antigen-unmasking fluid. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Mouse anti Rat CD3 antibody, clone 1F4 recognizes rat CD3, a approximately 25 kDa antigen which is found on rat T-cells.
靶标信息
The CD3 complex, composed of gamma, delta, epsilon, and zeta subunits, is essential for the assembly, trafficking, and surface expression of the T cell receptor (TCR) complex. These subunits are structurally related members of the immunoglobulin superfamily and are encoded by closely linked genes on human chromosome 11. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, but not on B or NK cells. The CD3 subunits play a crucial role in transducing antigen-recognition signals into the cytoplasm of T cells. The cytoplasmic tails of CD3 subunits contain a double tyrosine-based motif that associates with cytoplasmic signal transduction molecules, mediating T cell activation through the TCR. Crosslinking of the TCR initiates intracellular biochemical pathways that result in cellular activation, proliferation, and potentially growth arrest and cell survival. CD3 is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes, and Purkinje cells in the cerebellum. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency, highlighting its importance in immune function and regulation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD247 antigen; CD3 antigen delta polypeptide; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 gamma-chain; CD3 molecule delta polypeptide; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma polypeptide; CD3 TCR complex; CD3d; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex
基因别名: Cd3d; Cd3g; Cd3z; T3d; TCRzeta
UniProt ID: (Rat) P19377, (Rat) Q64159
Entrez Gene ID: (Rat) 25300, (Rat) 25710, (Rat) 315609, (Rat) 300678
cell surface receptor signaling pathway
T cell receptor signaling pathway
biological_process
T cell differentiation
positive thymic T cell selection
positive regulation of transcription from RNA polymerase II promoter
positive regulation of T cell anergy
response to nutrient
T cell costimulation
positive regulation of interferon-gamma production
positive regulation of interleukin-4 production
T cell differentiation in thymus
positive regulation of T cell proliferation
negative thymic T cell selection
positive regulation of interleukin-2 biosynthetic process
negative regulation of smoothened signaling pathway
positive regulation of alpha-beta T cell proliferation
lymphocyte activation
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of calcium-mediated signaling
positive regulation of T cell activation
apoptotic signaling pathway
establishment or maintenance of cell polarity
protein transport
regulation of lymphocyte apoptotic process
