Invitrogen

CD31 Monoclonal Antibody (TLD-3A12)

高级验证

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

1 篇参考文献

查看其他104个CD31 (PECAM-1)抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

Product Image Gallery

CD31 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Platelet endothelial cell adhesion molecule was performed using 70% confluent log phase HUVEC cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD31 Monoclonal Antibody (TLD-3A12) (Product # MA5-16951) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing Cell Junctional localization. Panel e represents no expression in MDA-MB-231. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

CD31 Antibody in Western Blot (WB) — Western blot was performed using Anti-CD31 Monoclonal Antibody (TLD-3A12) (Product # MA5-16951) and a 130kDa band corresponding to Platelet endothelial cell adhesion molecule was observed across cell lines tested. Membrane enriched extracts (40 µg lysate) of HUVEC (Lane 1), HEL 92.1.7 (Lane 2), U-937 (Lane 3), Jurkat (Lane 4), MDA-MB-231 (Lane 5), THP-1 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

CD31 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of Platelet endothelial cell adhesion molecule was observed to be high in HUVEC in comparison to no expression in MDA-MB-231 using Anti-CD31 Monoclonal Antibody (TLD-3A12) (Product # MA5-16951) in Western Blot (Ref :https://www.nature.com/articles/onc2013214). {RE}

产品信息

MA5-16951

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:1,000	-
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫组化（冰冻） (IHC (F))	Assay-dependent	-
免疫细胞化学 (ICC/IF)	1:100	-
流式细胞分析 (Flow)	1:50-1:100	-
酶联免疫吸附实验 (ELISA)	Assay-dependent	-

产品规格
种属反应	Non-human primate, Rat
已发表种属	Rat
宿主/亚型	Mouse / IgG1
分类	Monoclonal
类型	Antibody
克隆号	TLD-3A12
抗原	Activated, Lewis rat derived microglial cells
偶联物	Unconjugated Biotin FITC PE
形式	Liquid
浓度	1 mg/mL
纯化类型	Protein G
保存液	PBS
内含物	no preservative
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2538426

产品详细信息

This product is a low-endotoxin, preservative-free formulation. Endotoxin level is <0.01 EU/ug.

For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

靶标信息

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Erythropoietin protects against doxorubicin-induced heart failure.

American journal of physiology. Heart and circulatory physiology

Ammar HI,Saba S,Ammar RI,Elsayed LA,Ghaly WB,Dhingra S

MA5-16951 was used in immunohistochemistry to study the ability of erythropoeitin to protect against heart failure induced by treatment with the cancer drug doxorubicin

Thu Dec 01 00:00:00 EST 2011

生物信息学

蛋白别名: CD31; PECAM-1; Platelet endothelial cell adhesion molecule

基因别名: CD31; Pecam; Pecam1

UniProt ID: (Rat) Q3SWT0

Entrez Gene ID: (Rat) 29583

分子生物学功能: immunoglobulin receptor superfamily

功能

positive regulation of leukocyte migration phagocytosis cell adhesion Rho protein signal transduction regulation of cell migration negative regulation of actin filament polymerization response to cytokine negative regulation of GTPase activity wound healing positive regulation of tyrosine phosphorylation of Stat5 protein negative regulation of peptidyl-tyrosine phosphorylation diapedesis glomerular endothelium development

参与通路

protein binding

CD31 Monoclonal Antibody (TLD-3A12)

产品信息

MA5-16951

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Brands

Shopping Tools

eSolutions

Support

我们的公司

Your items have has been added!