Invitrogen

CD325 (N-Cadherin) Monoclonal Antibody (8C11), PE-Cyanine7, eBioscience™

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CD325 (N-Cadherin) Antibody in Flow Cytometry (Flow) — HeLa cells were stained with Mouse IgG1 kappa Isotype Control, PE-Cyanine7 (Product # 25-4714-80) (blue histogram) or CD325 Monoclonal Antibody, PE-Cyanine7 (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).

产品信息

25-3259-42

应用	建议稀释比	已发表文章
流式细胞分析 (Flow)	5 µL (0.25 µg)/test	-

产品规格
种属反应	Human
宿主/亚型	Mouse / IgG1, kappa
推荐的同型对照	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine7, eBioscience™
分类	Monoclonal
类型	Antibody
克隆号	8C11
偶联物	PE-Cyanine7 APC Biotin PE PerCP-eFluor 710 Unconjugated
激发/发射光谱	569/780 nm 查看光谱
形式	Liquid
浓度	5 µL/Test
纯化类型	Affinity chromatography
保存液	PBS, pH 7.2, with 0.2% BSA
内含物	0.09% sodium azide
保存条件	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2784666

产品详细信息

Description: The 8C11 monoclonal antibody reacts with human CD325, also known as N-Cadherin. CD325 is a 130 kDa member of the Cadherin superfamily, and consists of five extracellular repeats, a transmembrane domain and a cytoplasmic domain. CD325 deficient mice die at day 10 of gestation and embryos display major heart defects and malformed neural tubes and somites. Consistent with this, CD325 has been implicated in several aspects of cardiac development including the precardiac mesoderm, establishment of left-right symmetry and cardiac looping morphogenesis. Furthermore, CD325 is normally involved in inducing cell cycle arrest and its expression is frequently dysregulated in cancer cells.

Applications Reported: This 8C11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 8C11 antibody has been pre-diluted and tested by flow cytometric analysis of HeLa cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser

靶标信息

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; Cadherin2; calcium-dependent adhesion protein, neuronal; CD325; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; Neural cadherin

基因别名: CD325; CDH2; CDHN; CDw325; NCAD

UniProt ID: (Human) P19022

Entrez Gene ID: (Human) 1000

分子生物学功能: cadherin

功能

cell adhesion homophilic cell adhesion via plasma membrane adhesion molecules heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules glial cell differentiation calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules cell migration cerebral cortex development regulation of myelination adherens junction organization regulation of Rho protein signal transduction positive regulation of MAPK cascade cell-cell adhesion mediated by cadherin blood vessel morphogenesis brain morphogenesis homeostasis of number of cells regulation of axonogenesis striated muscle cell differentiation positive regulation of muscle cell differentiation protein heterooligomerization radial glial cell differentiation neuroepithelial cell differentiation regulation of oligodendrocyte progenitor proliferation establishment of protein localization to plasma membrane negative regulation of canonical Wnt signaling pathway neuroligin clustering involved in postsynaptic membrane assembly neuronal stem cell population maintenance regulation of postsynaptic density protein 95 clustering positive regulation of synaptic vesicle clustering

参与通路

calcium ion binding protein binding beta-catenin binding protein kinase binding protein phosphatase binding protein complex binding alpha-catenin binding gamma-catenin binding nitric-oxide synthase binding

CD325 (N-Cadherin) Monoclonal Antibody (8C11), PE-Cyanine7, eBioscience™

产品信息

25-3259-42

流式细胞分析 (Flow)

种属反应

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

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生物信息学

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