Invitrogen

CD32b Monoclonal Antibody (AT130-2), APC, eBioscience™

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CD32b Antibody (17-0321-82) in Flow

Staining of mouse splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.25 µg of Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (left) or 0.25 µg of Anti-Mouse CD32b APC (right). Total viable cells were used for analysis.

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产品信息

17-0321-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Mouse / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

AT130-2

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2573142

产品详细信息

Description: The monoclonal antibody AT130-2 reacts with mouse CD32b, also known as Fc-gamma receptor IIb. CD32b is the only inhibitory member of the Fc-gamma receptor family. Immune complex engagement of CD32b results in the phosphorylation of its intracellular ITIM motif. This engagement serves to attenuate B cell receptor (BCR) signaling and other activating signals, reduces proliferation, reduces antibody production, and may also deliver apoptotic signals. CD32b is the only Fc-gamma receptor expressed by B cells. CD32b is also expressed on monocytes, dendritic cells, neutrophils, eosinophils and basophils, as well as B cell progenitors and plasma cells.

AT130-2 has been reported to have agonist activity.

Applications Reported: This AT130-2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AT130-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

Optimal target saturation of ligand-blocking anti-GITR antibody IBI37G5 dictates FcγR-independent GITR agonism and antitumor activity.

Cell reports. Medicine

Liu H,Wu W,Sun G,Chia T,Cao L,Liu X,Guan J,Fu F,Yao Y,Wu Z,Zhou S,Wang J,Lu J,Kuang Z,Wu M,He L,Shao Z,Wu D,Chen B,Xu W,Wang Z,He K

17-0321-82 was used in Flow cytometry/Cell sorting to propose an alternative dose-finding strategy that does not rely on the traditional maximal tolerated dose (MTD)-based paradigm but instead on utilizing the RO-function relations as biomarker to guide the clinical translation of GITR and similar co-stimulatory agonists.

Tue Jun 21 00:00:00 EDT 2022

Cells of the human intestinal tract mapped across space and time.

Nature

Elmentaite R,Kumasaka N,Roberts K,Fleming A,Dann E,King HW,Kleshchevnikov V,Dabrowska M,Pritchard S,Bolt L,Vieira SF,Mamanova L,Huang N,Perrone F,Goh Kai'En I,Lisgo SN,Katan M,Leonard S,Oliver TRW,Hook CE,Nayak K,Campos LS,Domínguez Conde C,Stephenson E,Engelbert J,Botting RA,Polanski K,van Dongen S,Patel M,Morgan MD,Marioni JC,Bayraktar OA,Meyer KB,He X,Barker RA,Uhlig HH,Mahbubani KT,Saeb-Parsy K,Zilbauer M,Clatworthy MR,Haniffa M,James KR,Teichmann SA

Published figure using CD32b monoclonal antibody (Product # 17-0321-82) in Flow Cytometry

Wed Sep 01 00:00:00 EDT 2021

Signaling through the Inhibitory Fc Receptor FcγRIIB Induces CD8⁺ T Cell Apoptosis to Limit T Cell Immunity.

Immunity

Morris AB,Farley CR,Pinelli DF,Adams LE,Cragg MS,Boss JM,Scharer CD,Fribourg M,Cravedi P,Heeger PS,Ford ML

17-0321-82 was used in Flow Cytometry to demonstrate a cell-intrinsic coinhibitory function of FcγRIIB in regulating CD8+ T cell immunity.

Tue Jan 14 00:00:00 EST 2020

Optimization of 4-1BB antibody for cancer immunotherapy by balancing agonistic strength with FcγR affinity.

Nature communications

Qi X,Li F,Wu Y,Cheng C,Han P,Wang J,Yang X

17-0321-82 was used in Flow Cytometry to develop LVGN6051, a humanized 4-1BB mAb-AG that shows high anti-tumor efficacy in the absence of liver toxicity in a mouse model of cancer immunotherapy.

Mon May 20 00:00:00 EDT 2019

生物信息学

蛋白别名: CD32; CD32 receptor 2; CD32 receptor 2a; CD32 receptor 2b; CD32A; Fc gamma receptor IIB; Fc gamma RIIB; Fc-gamma RII; fc-gamma RII-a; Fc-gamma-RII; fc-gamma-RIIa; fc-gamma-RIIB; FcgammaRIIB; FcGR; FCGR2A1; fcRII-a; FGFR2B; IgG Fc receptor II beta; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; Ly-17; Lymphocyte antigen 17

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基因别名: AI528646; CD32; F630109E10Rik; Fc[g]RII; Fcgr2; Fcgr2a; Fcgr2b; FcgRII; Fcr-2; Fcr-3; fcRII; Ly-17; Ly-m20; LyM-1

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UniProt ID: (Mouse) P08101

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Entrez Gene ID: (Mouse) 14130

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功能

receptor signaling protein activity protein binding IgG binding immunoglobulin binding immunoglobulin receptor superfamily

参与通路