Invitrogen

CD335 (NKp46) Monoclonal Antibody (29A1.4), Super Bright™ 436, eBioscience™

Product Image Gallery

CD335 (NKp46) Antibody in Flow Cytometry (Flow) — BALB/c mouse splenocytes were stained with CD49b Monoclonal Antibody, APC (Product # 17-5971-82) and 1.0 µg of Rat IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4321-82) (left) or 1.0 µg of CD335 (NKp46) Monoclonal Antibody, Super Bright 436 (right). Cells in the lymphocyte gate were used for analysis.

CD335 (NKp46) Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Antibody-mediated blocking of CD44 increases heCIC formation and immune killing. a Representative cytospin images for heCIC formation (arrows) in F6ft (green)--CCRF-CEM (red) co-culture treated with control IgG or Hermes-1, a CD44 blocking antibody. Scale bar, 50 um. b , c Quantification of heCIC formation between F6ft and CCRF-CEM ( b ), or NK92MI ( c ). F6ft cells were pretreated with Hermes-1 for 1 h followed by co-culture for 8 h with either CCRF-CEM cells or NK92MI cells. Data are means +- SD of six images of 20x objective. n > 800 cells analyzed for each cell. ** P < 0.01, *** P < 0.001, **** P < 0.0001. d Representative images for tumor cell killing by NK92MI cells in the presence of different amounts of Hermes-1 antibody. Scale bar, 50 um. e Tumor cell killing by NK92MI cells was promoted by Hermes-1 (Her) treatment. Cells were co-cultured for 8 h (E/T = 4:1) before measured by Cell Counting Kit-8. * P < 0.05; **** P < 0.0001. Data are means +- SD of triplicate assays. f , g Hermes-1 (Her) treatment had little influence on the autonomous cell growth of either NK92MI ( f ) or F6ft cells ( g ). h Representative images of heCIC structures formed between F6ft cells and NK92MI cells in xenograft tumor tissues stained with antibodies against E-cadherin (green) and NKp46 (red). Scale bar, 15 um. i Increased heCIC formation in xenograft tumor tissue treated with Hermes-1. Data are means +- SD of six images of 20x objective. **** P < 0.0001. j Four groups of tumors xenografts

产品信息

62-3351-82

应用	建议稀释比	已发表文章
免疫组化（石蜡） (IHC (P))	-	查看1篇已发表文章 1篇已发表文章
流式细胞分析 (Flow)	1.0 µg/test	查看4篇已发表文章 4篇已发表文章

产品规格
种属反应	Mouse
已发表种属	Not Applicable
宿主/亚型	Rat / IgG2a, kappa
推荐的同型对照	Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™
分类	Monoclonal
类型	Antibody
克隆号	29A1.4
偶联物	Super Bright™ 436 Alexa Fluor 700 APC APC-eFluor 780 Biotin Brilliant Violet 711 eFluor 450 eFluor 660 FITC Functional Grade PE PE-Cyanine7 PE-eFluor 610 PerCP-eFluor 710 Super Bright 600 Super Bright 645
激发/发射光谱	413/431 nm 查看光谱
形式	Liquid
浓度	0.2 mg/mL
纯化类型	Affinity chromatography
保存液	PBS, pH 7.2, with BSA
内含物	0.09% sodium azide
保存条件	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2744798

产品详细信息

Description: The monoclonal antibody 29A1.4 recognizes mouse NKp46, also known as CD335. CD335, a member of the natural cytotoxicity receptor (NCR) family, is a glycoprotein with 2 Ig-like domains and a short cytoplasmic tail. Expression of CD335 is uniquely found on NK cells (including immature NK cells, defined as DX5- CD3-, and thereby allowing discrimination between NKT cells and NK cells (NKp46+, CD3-). Furthermore, unlike many of the NK markers which also stain NKT cells, staining with 29A1.4 is not strain specific. Staining has been shown on C57Bl/6, SJL, CBA/CA and BALB/C strains. NKp46 has been shown to play a role in NK cell-mediated lysis of several tumor cells and pathogen-infected cell lines.

The 29A1.4 monoclonal antibody has been shown to activate NK cells in vitro. The 29A1.4 monoclonal antibody does not deplete NK cells in vivo.

Applications Reported: This 29A1.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 29A1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

靶标信息

NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Flow Cytometry

Panel Builder

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Cell-in-cell structure mediates in-cell killing suppressed by CD44.

Cell discovery

Su Y,Huang H,Luo T,Zheng Y,Fan J,Ren H,Tang M,Niu Z,Wang C,Wang Y,Zhang Z,Liang J,Ruan B,Gao L,Chen Z,Melino G,Wang X,Sun Q

Published figure using CD335 (NKp46) monoclonal antibody (Product # 62-3351-82) in Immunohistochemistry (Paraffin)

Tue Apr 19 00:00:00 EDT 2022

NK cells in hypoxic skin mediate a trade-off between wound healing and antibacterial defence.

Nature communications

Sobecki M,Krzywinska E,Nagarajan S,Audigé A,Hu¿nh K,Zacharjasz J,Debbache J,Kerdiles Y,Gotthardt D,Takeda N,Fandrey J,Sommer L,Sexl V,Stockmann C

Published figure using CD335 (NKp46) monoclonal antibody (Product # 62-3351-82) in Flow Cytometry

Wed Aug 04 00:00:00 EDT 2021

Characterization of the pathoimmunology of necrotizing enterocolitis reveals novel therapeutic opportunities.

Nature communications

Cho SX,Rudloff I,Lao JC,Pang MA,Goldberg R,Bui CB,McLean CA,Stock M,Klassert TE,Slevogt H,Mangan NE,Cheng W,Fischer D,Gfroerer S,Sandhu MK,Ngo D,Bujotzek A,Lariviere L,Schumacher F,Tiefenthaler G,Beker F,Collins C,Kamlin COF,König K,Malhotra A,Tan K,Theda C,Veldman A,Ellisdon AM,Whisstock JC,Berger PJ,Nold-Petry CA,Nold MF

Published figure using CD335 (NKp46) monoclonal antibody (Product # 62-3351-82) in Flow Cytometry

Fri Nov 13 00:00:00 EST 2020

Synergized regulation of NK cell education by NKG2A and specific Ly49 family members.

Nature communications

Zhang X,Feng J,Chen S,Yang H,Dong Z

Published figure using CD335 (NKp46) monoclonal antibody (Product # 62-3351-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

SCGN deficiency results in colitis susceptibility.

eLife

Sifuentes-Dominguez LF,Li H,Llano E,Liu Z,Singla A,Patel AS,Kathania M,Khoury A,Norris N,Rios JJ,Starokadomskyy P,Park JY,Gopal P,Liu Q,Tan S,Chan L,Ross T,Harrison S,Venuprasad K,Baker LA,Jia D,Burstein E

Published figure using CD335 (NKp46) monoclonal antibody (Product # 62-3351-82) in Flow Cytometry

Wed Oct 30 00:00:00 EDT 2019

生物信息学

蛋白别名: 1-Mar; Activating receptor 1; CD335; hNKp46; Ly-94; Lymphocyte antigen 94; mAR-1; MAR1 (mouse activating receptor 1); mNKp46; Natural cytotoxicity triggering receptor 1; Natural killer cell p46-related protein; natural killer cell receptor; NK-p46

基因别名: Ly94; Ncr1; NKp46

UniProt ID: (Mouse) Q8C567

Entrez Gene ID: (Mouse) 17086

分子生物学功能: immunoglobulin receptor superfamily

功能

detection of virus defense response to virus

CD335 (NKp46) Monoclonal Antibody (29A1.4), Super Bright™ 436, eBioscience™

产品信息

62-3351-82

免疫组化（石蜡） (IHC (P))

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

随时为您服务

Your items have has been added!