Invitrogen

CD38 Monoclonal Antibody (HIT2), eBioscience™

CD38 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD38 Antibody (14-0389-82) in IHC (P)

Immunohistochemistry of formalin fixed paraffin embedded human small intestine stained with 40 µg/mL Anti-Human CD38 Purified followed by Anti-Mouse IgG Biotin, Streptavidin Alkaline Phosphatase and Fast Red. Nuclei are counterstained with hematoxylin.

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产品信息

14-0389-82

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

40 µg/mL

流式细胞分析 (Flow)

1 µg/test

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酶联免疫吸附实验 (ELISA)

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_467222

产品详细信息

Description: The HIT2 monoclonal antibody reacts with the human CD38 molecule, an approximately 45 kDa type II transmembrane protein. CD38 is expressed by thymocytes, peripheral B cells including plasma cells, activated T cells, and monocytes. CD38 is a counter-receptor for CD31 and has both cyclase and hydrolase enzymatic activity.

Applications Reported: The HIT2 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining.

Applications Tested: The HIT2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The HIT2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retreival and can be used at less than or equal to 40 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

CD38 is a multifunctional ectoenzyme and type II transmembrane glycoprotein involved in various cellular processes. It catalyzes the conversion of NAD into secondary messengers such as nicotinic acid adenine dinucleotide phosphate (NAADP) and cyclic ADP-ribose (cADPR). CD38 plays a crucial role in B cell development, with expression levels fluctuating from high in immature cells to low in intermediate ones, and back to high in mature B cells. It is also present in a variety of tissues and hematopoietic cells, including T cells, NK cells, and monocytes, and is used to phenotype leukemias and monitor HIV-1 progression. The CD34+CD38- population is considered to define the most pluripotent hematopoietic stem cells. In addition to its surface expression, CD38 has been identified in the nucleus, where it may regulate calcium levels. It functions as a multi-catalytic ectoenzyme, serving roles as an ADP-ribosyl cyclase, cyclic ADP-ribose hydrolase, and possibly NAD+ glycohydrolase, or as a cell surface receptor. CD38 is involved in the activation, proliferation, and differentiation of mature lymphocytes and mediates apoptosis of myeloid and lymphoid progenitor cells. It also participates in cell adhesion, signal transduction, and calcium signaling. Antibodies to CD38 are valuable for subtyping lymphomas and leukemias, detecting plasma cells (such as in myelomas), and marking activated B and T cells. CD38 is expressed at high levels in the pancreas, liver, kidney, malignant lymphoma, and neuroblastoma. Dysfunctions in CD38 are associated with diseases like chronic lymphocytic leukemia and Richter's Syndrome.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Immunometabolic interference between cancer and COVID-19.

Frontiers in immunology

Consonni FM,Durante B,Manfredi M,Bleve A,Pandolfo C,Garlatti V,Vanella VV,Marengo E,Barberis E,Bottazzi B,Bombace S,My I,Condorelli G,Torri V,Sica A

Published figure using CD38 monoclonal antibody (Product # 14-0389-82) in Flow Cytometry

Tue Apr 18 00:00:00 UTC 2023

B cell-intrinsic changes with age do not impact antibody-secreting cell formation but delay B cell participation in the germinal centre reaction.

Aging cell

Lee JL,Fra-Bido SC,Burton AR,Innocentin S,Hill DL,Linterman MA

Published figure using CD38 monoclonal antibody (Product # 14-0389-82) in Flow Cytometry

Thu Sep 01 00:00:00 UTC 2022

Novel myeloma patient-derived xenograft models unveil the potency of anlotinib to overcome bortezomib resistance.

Frontiers in oncology

Yue Y,Cao Y,Mao X,Wang F,Fan P,Qian L,Guo S,Li F,Guo Y,Chen T,Lin Y,Dong W,Liu Y,Huang Y,Gu W

Published figure using CD38 monoclonal antibody (Product # 14-0389-82) in Flow Cytometry

Tue Aug 23 00:00:00 UTC 2022

Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions.

Immunity

Schwabenland M,Salié H,Tanevski J,Killmer S,Lago MS,Schlaak AE,Mayer L,Matschke J,Püschel K,Fitzek A,Ondruschka B,Mei HE,Boettler T,Neumann-Haefelin C,Hofmann M,Breithaupt A,Genc N,Stadelmann C,Saez-Rodriguez J,Bronsert P,Knobeloch KP,Blank T,Thimme R,Glatzel M,Prinz M,Bengsch B

14-0389-82 was used in Mass cytometry to identify profound neuroinflammation with activation of innate and adaptive immune cells as correlates of COVID-19 neuropathology, with implications for potential therapeutic strategies.

Tue Jul 13 00:00:00 UTC 2021

Kaposi's sarcoma-associated herpesvirus latency-associated nuclear antigen dysregulates expression of MCL-1 by targeting FBW7.

PLoS pathogens

Kim YJ,Kim Y,Kumar A,Kim CW,Toth Z,Cho NH,Lee HR

Published figure using CD38 monoclonal antibody (Product # 14-0389-82) in Flow Cytometry

Fri Jan 01 00:00:00 UTC 2021

Highly efficient CRISPR-Cas9-mediated gene knockout in primary human B cells for functional genetic studies of Epstein-Barr virus infection.

PLoS pathogens

Akidil E,Albanese M,Buschle A,Ruhle A,Pich D,Keppler OT,Hammerschmidt W

Published figure using CD38 monoclonal antibody (Product # 14-0389-82) in Flow Cytometry

Thu Apr 01 00:00:00 UTC 2021

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

Published figure using CD38 monoclonal antibody (Product # 14-0389-82) in Flow Cytometry

Tue Jun 01 00:00:00 UTC 2021

Enhanced CXCR4 Expression of Human CD8^Low T Lymphocytes Is Driven by S1P₄.

Frontiers in immunology

Burkard T,Dreis C,Herrero San Juan M,Huhn M,Weigert A,Pfeilschifter JM,Radeke HH

Published figure using CD38 monoclonal antibody (Product # 14-0389-82) in Flow Cytometry

Wed Oct 20 00:00:00 UTC 2021

Synthesis of site-specific antibody-drug conjugates by ADP-ribosyl cyclases.

Science advances

Dai Z,Zhang XN,Nasertorabi F,Cheng Q,Li J,Katz BB,Smbatyan G,Pei H,Louie SG,Lenz HJ,Stevens RC,Zhang Y

Published figure using CD38 monoclonal antibody (Product # 14-0389-82) in ELISA

Mon Jun 01 00:00:00 UTC 2020

Chronic myelomonocytic leukaemia stem cell transcriptomes anticipate disease morphology and outcome.

EBioMedicine

Wiseman DH,Baker SM,Dongre AV,Gurashi K,Storer JA,Somervaille TC,Batta K

Published figure using CD38 monoclonal antibody (Product # 14-0389-82) in Flow Cytometry

Sat Aug 01 00:00:00 UTC 2020

IFNγ induces epigenetic programming of human T-bet^hi B cells and promotes TLR7/8 and IL-21 induced differentiation.

eLife

Zumaquero E,Stone SL,Scharer CD,Jenks SA,Nellore A,Mousseau B,Rosal-Vela A,Botta D,Bradley JE,Wojciechowski W,Ptacek T,Danila MI,Edberg JC,Bridges SL,Kimberly RP,Chatham WW,Schoeb TR,Rosenberg AF,Boss JM,Sanz I,Lund FE

Published figure using CD38 monoclonal antibody (Product # 14-0389-82) in Flow Cytometry

Wed May 15 00:00:00 UTC 2019

Relative safety of various spermatogenic stem cell purification methods for application in spermatogenic stem cell transplantation.

Stem cell research & therapy

Tian J,Ma K,Pei CB,Zhang SH,Li X,Zhou Y,Yan B,Wang HY,Ma LH

14-0389-82 was used in Flow Cytometry, Immunohistochemistry (Paraffin) to confirm that immunomagnetic beads and flow cytometry methods of purifying SSCs from the testicular tissue of the testicular leukemia mouse model could be safely applied to the SSC transplantation technology without concomitant tumor implantation.

Mon Dec 16 00:00:00 UTC 2019

Characteristics of doxorubicin-selected multidrug-resistant human leukemia HL-60 cells with tolerance to arsenic trioxide and contribution of leukemia stem cells.

Oncology letters

Chen J,Wei H,Cheng J,Xie B,Wang B,Yi J,Tian B,Liu Z,Wang F,Zhang Z

14-0389-82 was used in Flow Cytometry to select and characterized a multidrug-resistant HL-60 human acute promyelocytic leukemia cell line, HL-60/RS, by exposure to stepwise incremental doses of doxorubicin.

Mon Jan 01 00:00:00 UTC 2018

Abnormal phenotypic features of IgM+B cell subsets in patients with chronic hepatitis C virus infection.

Experimental and therapeutic medicine

Kong F,Feng B,Zhang H,Rao H,Wang J,Cong X,Wei L

Published figure using CD38 monoclonal antibody (Product # 14-0389-82) in Flow Cytometry

Tue Aug 01 00:00:00 UTC 2017

Brief Report: IFIH1 Mutation Causes Systemic Lupus Erythematosus With Selective IgA Deficiency.

Arthritis & rheumatology (Hoboken, N.J.)

Van Eyck L,De Somer L,Pombal D,Bornschein S,Frans G,Humblet-Baron S,Moens L,de Zegher F,Bossuyt X,Wouters C,Liston A

14-0389 was used in Flow cytometry/Cell sorting to determine the underlying genetic defect responsible for severe early onset and refractory systemic lupus erythematosus.

Mon Jun 01 00:00:00 UTC 2015

Development of a diverse human T-cell repertoire despite stringent restriction of hematopoietic clonality in the thymus.

Proceedings of the National Academy of Sciences of the United States of America

Brugman MH,Wiekmeijer AS,van Eggermond M,Wolvers-Tettero I,Langerak AW,de Haas EF,Bystrykh LV,van Rood JJ,de Haan G,Fibbe WE,Staal FJ

14-0389 was used in Flow cytometry/Cell sorting to study T-cell development in the thymus at a clonal level, with an aim to improve immune reconstitution following hematopoietic stem cell transplant.

Tue Nov 03 00:00:00 UTC 2015

Higher Frequency of NK and CD4+ T-Cells in Mucosa and Potent Cytotoxic Response in HIV Controllers.

PloS one

Taborda NA,González SM,Alvarez CM,Correa LA,Montoya CJ,Rugeles MT

14-0389 was used in Flow cytometry/Cell sorting to investigate the frequency, phenotype and activity of T and NK cells in HIV controllers, showing higher frequencies of NK and CD4+ T-Cells and potent cytotoxic response.

Fri May 13 00:00:00 UTC 2016

Regulatory B cells are numerically but not functionally deficient in anti-neutrophil cytoplasm antibody-associated vasculitis.

Rheumatology (Oxford, England)

Todd SK,Pepper RJ,Draibe J,Tanna A,Pusey CD,Mauri C,Salama AD

14-0389 was used in Flow cytometry/Cell sorting to study the frequencies of different B cell subtypes during progression of ANCA-associated vasculitis.

Mon Sep 01 00:00:00 UTC 2014

Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.

Stem cells (Dayton, Ohio)

Fernandez I,Ooi TP,Roy K

Published figure using CD38 monoclonal antibody (Product # 14-0389-82) in Flow Cytometry

Wed Jan 01 00:00:00 UTC 2014

Generation of functional, antigen-specific CD8+ human T cells from cord blood stem cells using exogenous Notch and tetramer-TCR signaling.

Stem cells (Dayton, Ohio)

Fernandez I,Ooi TP,Roy K

Published figure using CD38 monoclonal antibody (Product # 14-0389-82) in Flow Cytometry

Wed Jan 01 00:00:00 UTC 2014

PDGFRα and CD51 mark human nestin+ sphere-forming mesenchymal stem cells capable of hematopoietic progenitor cell expansion.

The Journal of experimental medicine

Pinho S,Lacombe J,Hanoun M,Mizoguchi T,Bruns I,Kunisaki Y,Frenette PS

14-0389 was used in Immunofluorescence to evaluate putative cell surface mesenchymal stem cell markers to identify Nestin+ cells in the mouse and human bone marrow.

Mon Jul 01 00:00:00 UTC 2013

In vivo expansion of co-transplanted T cells impacts on tumor re-initiating activity of human acute myeloid leukemia in NSG mice.

PloS one

von Bonin M,Wermke M,Cosgun KN,Thiede C,Bornhauser M,Wagemaker G,Waskow C

14-0389 was used in Flow cytometry/Cell sorting to investigate the mechanisms behind the limitations of xenotransplantation assays of cells from leukaemia patients into immune compromised mice.

Tue Oct 22 00:00:00 UTC 2013

Targeting CDK1 promotes FLT3-activated acute myeloid leukemia differentiation through C/EBPα.

The Journal of clinical investigation

Radomska HS,Alberich-Jordà M,Will B,Gonzalez D,Delwel R,Tenen DG

Published figure using CD38 monoclonal antibody (Product # 14-0389-82) in Flow Cytometry

Wed Aug 01 00:00:00 UTC 2012

B cell repopulation after alemtuzumab induction-transient increase in transitional B cells and long-term dominance of naïve B cells.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Heidt S,Hester J,Shankar S,Friend PJ,Wood KJ

14-0389 was used in Flow cytometry/Cell sorting to investigate the characteristics of B cells that repopulate following Alemtuzumab induction therapy.

Sun Jul 01 00:00:00 UTC 2012

生物信息学

蛋白别名: 2'-phospho-ADP-ribosyl cyclase; 2'-phospho-ADP-ribosyl cyclase/2'-phospho-cyclic-ADP-ribose transferase; 2'-phospho-cyclic-ADP-ribose transferase; ADP-ribosyl cyclase 1; ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 1; ADPRC 1; cADPR hydrolase 1; CD38; CD38 antigen (p45); CD38 antigen p45; cluster of differentiation 38; Cyclic ADP-ribose hydrolase 1; ecto-nicotinamide adenine dinucleotide glycohydrolase; NAD(+) nucleosidase; NAD+ nucleosidase; T10

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基因别名: ADPRC 1; ADPRC1; CD38

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UniProt ID: (Human) Q96HY4

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Entrez Gene ID: (Human) 952

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功能

NAD+ nucleosidase activity transferase activity phosphorus-oxygen lyase activity NAD(P)+ nucleosidase activity cyclase lyase metabolite interconversion enzyme

参与通路