Invitrogen

CD3e Monoclonal Antibody (145-2C11), PerCP-Cyanine5.5, eBioscience™

Product Image Gallery

CD3e Antibody in Flow Cytometry (Flow) — Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and staining buffer (autofluorescence) (left) or 0.5 µg of Anti-Mouse CD3e PerCP-Cyanine5-5 (right). Total viable cells were used for analysis.

产品信息

45-0031-82

应用	建议稀释比	已发表文章
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫组化（石蜡） (IHC (P))	-	查看1篇已发表文章 1篇已发表文章
免疫组化（冰冻） (IHC (F))	-	查看2篇已发表文章 2篇已发表文章
流式细胞分析 (Flow)	1 µg/test	查看26篇已发表文章 26篇已发表文章
功能检测 (FN)	-	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Mouse
已发表种属	Mouse
宿主/亚型	Armenian hamster / IgG
推荐的同型对照	Armenian Hamster IgG Isotype Control (eBio299Arm), PerCP-Cyanine5.5, eBioscience™
分类	Monoclonal
类型	Antibody
克隆号	145-2C11
偶联物	PerCP-Cyanine5.5 Alexa Fluor 488 APC APC-eFluor 780 Biotin Brilliant UV 737 eFluor 450 FITC Functional Grade NovaFluor Blue 510 NovaFluor Blue 530 NovaFluor Blue 555 NovaFluor Blue 585 NovaFluor Blue 610-30S NovaFluor Blue 610-70S NovaFluor Blue 660-120S NovaFluor Blue 660-40S NovaFluor Red 660 NovaFluor Red 685 NovaFluor Red 700 NovaFluor Red 710 NovaFluor Yellow 570 NovaFluor Yellow 590 NovaFluor Yellow 610 NovaFluor Yellow 660
激发/发射光谱	查看光谱
形式	Liquid
浓度	0.2 mg/mL
纯化类型	Affinity chromatography
保存液	PBS, pH 7.2
内含物	0.09% sodium azide
保存条件	4° C, store in dark, DO NOT FREEZE!
RRID	AB_1107000

产品详细信息

Description: The 145-2C11 monoclonal antibody reacts with mouse CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits, gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Binding of 145-2C11 to TCR initiates the intracellular biochemical pathway resulting in cellular activation, proliferation, and apoptosis depending on specific conditions utilized. 145-2C11 is commonly used as a phenotypic marker for mouse T cells.

Applications Reported: This 145-2C11 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 145-2C11 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Flow Cytometry

Panel Builder

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Scientific reports

Tran A,Scholtes C,Songane M,Champagne C,Galarneau L,Levasseur MP,Fodil N,Dufour CR,Giguère V,Saleh M

45-0031-82 was used in Immunohistochemistry (Paraffin) to report a protective role of estrogen-related receptor alpha in the intestine.

Fri Jul 23 00:00:00 EDT 2021

C5aR1 Activation Drives Early IFN-γ Production to Control Experimental Toxoplasma gondii Infection.

Frontiers in immunology

Briukhovetska D,Ohm B,Mey FT,Aliberti J,Kleingarn M,Huber-Lang M,Karsten CM,Köhl J

45-0031 was used in Flow cytometry/Cell sorting to suggest a model in which the C5a/C5aR1 axis promotes IFN-γ production from NK cells as a mechanism to control acute Toxoplasma gondii infection.

Mon Apr 05 00:00:00 EDT 2021

The Interleukin (IL)-1R1 pathway is a critical negative regulator of PyMT-mediated mammary tumorigenesis and pulmonary metastasis.

Oncoimmunology

Dagenais M,Dupaul-Chicoine J,Douglas T,Champagne C,Morizot A,Saleh M

45-0031 was used in Flow cytometry/Cell sorting to genetically investigate the role of the interleukin-1 receptor 1 pathway in breast cancer tumourigenesis and metastasis using a mouse model.

Tue Jan 12 00:00:00 EST 2021

Ageing promotes early T follicular helper cell differentiation by modulating expression of RBPJ.

Aging cell

Webb LMC,Fra-Bido S,Innocentin S,Matheson LS,Attaf N,Bignon A,Novarino J,Fazilleau N,Linterman MA

45-0031-82 was used in Flow Cytometry to show increased formation of Tfh precursors (pre-Tfh) but no associated increase in germinal centre (GC)-Tfh cells in aged mice, suggesting age-driven promotion of only early Tfh cell differentiation.

Fri Jan 01 00:00:00 EST 2021

DEK-targeting aptamer DTA-64 attenuates bronchial EMT-mediated airway remodelling by suppressing TGF-β1/Smad, MAPK and PI3K signalling pathway in asthma.

Journal of cellular and molecular medicine

Song Y,Wang Z,Jiang J,Piao Y,Li L,Xu C,Piao H,Li L,Yan G

45-0031 was used in Flow cytometry/Cell sorting to study DEK-targeting aptamer in EMT-mediated airway remodeling in asthma.

Tue Dec 01 00:00:00 EST 2020

The Intermucosal Connection between the Mouth and Gut in Commensal Pathobiont-Driven Colitis.

Cell

Kitamoto S,Nagao-Kitamoto H,Jiao Y,Gillilland MG,Hayashi A,Imai J,Sugihara K,Miyoshi M,Brazil JC,Kuffa P,Hill BD,Rizvi SM,Wen F,Bishu S,Inohara N,Eaton KA,Nusrat A,Lei YL,Giannobile WV,Kamada N

45-0031-82 was used in Flow Cytometry to conclude that oral inflammation, such as periodontitis, exacerbates gut inflammation by supplying the gut with both colitogenic pathobionts and pathogenic T cells.

Thu Jul 23 00:00:00 EDT 2020

Diverse CD8 T Cell Responses to Viral Infection Revealed by the Collaborative Cross.

Cell reports

Martin MD,Sompallae R,Winborn CS,Harty JT,Badovinac VP

45-0031 was used in Flow cytometry/Cell sorting to advance the utility of the Collaborative Cross (CC) as a tool to analyze the immune response to viral infection.

Tue Apr 14 00:00:00 EDT 2020

Acid ceramidase of macrophages traps herpes simplex virus in multivesicular bodies and protects from severe disease.

Nature communications

Lang J,Bohn P,Bhat H,Jastrow H,Walkenfort B,Cansiz F,Fink J,Bauer M,Olszewski D,Ramos-Nascimento A,Duhan V,Friedrich SK,Becker KA,Krawczyk A,Edwards MJ,Burchert A,Huber M,Friebus-Kardash J,Göthert JR,Hardt C,Probst HC,Schumacher F,Köhrer K,Kleuser B,Babiychuk EB,Sodeik B,Seibel J,Greber UF,Lang PA,Gulbins E,Lang KS

45-0031-82 was used in Flow Cytometry to show that macrophages take up HSV-1 via endocytosis and transport the virions into multivesicular bodies (MVBs).

Thu Mar 12 00:00:00 EDT 2020

The Toxoplasma gondii virulence factor ROP16 acts in cis and trans, and suppresses T cell responses.

The Journal of experimental medicine

Chen L,Christian DA,Kochanowsky JA,Phan AT,Clark JT,Wang S,Berry C,Oh J,Chen X,Roos DS,Beiting DP,Koshy AA,Hunter CA

45-0031 was used in Flow cytometry/Cell sorting to investigate the impact of the ability of Toxoplasma gondii injecting the rhoptry kinase ROP16 into host cells on infection.

Mon Mar 02 00:00:00 EST 2020

Enhanced alleviation of aGVHD by TGF-β1-modified mesenchymal stem cells in mice through shifting MΦ into M2 phenotype and promoting the differentiation of Treg cells.

Journal of cellular and molecular medicine

Wu R,Liu C,Deng X,Chen L,Hao S,Ma L

45-0031 was used in Flow cytometry/Cell sorting to study the mechanism by which mesenchymal stem cells can treat steroid resistant acute graft-versus-host disease.

Wed Jan 01 00:00:00 EST 2020

Aging Induces an Nlrp3 Inflammasome-Dependent Expansion of Adipose B Cells That Impairs Metabolic Homeostasis.

Cell metabolism

Camell CD,Günther P,Lee A,Goldberg EL,Spadaro O,Youm YH,Bartke A,Hubbard GB,Ikeno Y,Ruddle NH,Schultze J,Dixit VD

45-0031 was used in Flow cytometry/Cell sorting to study the role of ageing within alterations of adipose tissue.

Tue Dec 03 00:00:00 EST 2019

Lymph Node Mesenchymal and Endothelial Stromal Cells Cooperate via the RANK-RANKL Cytokine Axis to Shape the Sinusoidal Macrophage Niche.

Immunity

Camara A,Cordeiro OG,Alloush F,Sponsel J,Chypre M,Onder L,Asano K,Tanaka M,Yagita H,Ludewig B,Flacher V,Mueller CG

45-0031 was used in Flow cytometry/Cell sorting to demonstrate that mesenchymal stromal cells created a niche environment with LECs for specialized macrophages to take up residence, complete their differentiation program, and fulfill their immune function.

Tue Jun 18 00:00:00 EDT 2019

Tumor-intrinsic response to IFNγ shapes the tumor microenvironment and anti-PD-1 response in NSCLC.

Life science alliance

Bullock BL,Kimball AK,Poczobutt JM,Neuwelt AJ,Li HY,Johnson AM,Kwak JW,Kleczko EK,Kaspar RE,Wagner EK,Hopp K,Schenk EL,Weiser-Evans MC,Clambey ET,Nemenoff RA

45-0031-82 was used in Flow Cytometry to demonstrate that targeted enhancement of tumor-intrinsic IFNγ signaling can induce a cascade of changes associated with increased therapeutic vulnerability.

Sat Jun 01 00:00:00 EDT 2019

Negative Co-stimulation Constrains T Cell Differentiation by Imposing Boundaries on Possible Cell States.

Immunity

Wei SC,Sharma R,Anang NAS,Levine JH,Zhao Y,Mancuso JJ,Setty M,Sharma P,Wang J,Pe'er D,Allison JP

45-0031 was used in Flow cytometry/Cell sorting to implicate negative co-stimulation as a key regulator and determinant of T cell differentiation and suggest that checkpoint blockade might work in part by altering the limits of T cell phenotypes.

Tue Apr 16 00:00:00 EDT 2019

Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing.

Nature communications

Sheng C,Yao C,Wang Z,Chen H,Zhao Y,Xu D,Huang H,Huang W,Chen S

45-0031 was used in Flow cytometry/Cell sorting to identify cyclophilin J as a negative feedback regulator of the NF-ΚB signalling pathway.

Mon Oct 22 00:00:00 EDT 2018

Complement Activation Contributes to Severe Acute Respiratory Syndrome Coronavirus Pathogenesis.

mBio

Gralinski LE,Sheahan TP,Morrison TE,Menachery VD,Jensen K,Leist SR,Whitmore A,Heise MT,Baric RS

45-0031 was used in Flow cytometry/Cell sorting to study complement signalling as regulating a proinflammatory response to SARS-CoV infection.

Tue Oct 09 00:00:00 EDT 2018

Expansion of commensal fungus Wallemia mellicola in the gastrointestinal mycobiota enhances the severity of allergic airway disease in mice.

PLoS pathogens

Skalski JH,Limon JJ,Sharma P,Gargus MD,Nguyen C,Tang J,Coelho AL,Hogaboam CM,Crother TR,Underhill DM

45-0031 was used in Flow cytometry/Cell sorting to investigate the effects of the population expansion of commensal fungus Wallemia mellicola without overgrowth of the total fungal community.

Sat Sep 01 00:00:00 EDT 2018

Stem cell factor is selectively secreted by arterial endothelial cells in bone marrow.

Nature communications

Xu C,Gao X,Wei Q,Nakahara F,Zimmerman SE,Mar J,Frenette PS

45-0031 was used in Flow cytometry/Cell sorting to characterise the expression signature that distinguishes arterial endothelial cells from sinusoidal endothelial cells.

Fri Jun 22 00:00:00 EDT 2018

Transcription factors early growth response gene (Egr) 2 and 3 control inflammatory responses of tolerant T cells.

Immunity, inflammation and disease

Omodho B,Miao T,Symonds ALJ,Singh R,Li S,Wang P

45-0031 was used in Flow cytometry/Cell sorting to show that despite impaired proliferation and IL2 production, tolerant T cells can display inflammatory responses in response to antigen stimulation and this is controlled at least partly by Egr2 and 3.

Fri Jun 01 00:00:00 EDT 2018

A cancer vaccine-mediated postoperative immunotherapy for recurrent and metastatic tumors.

Nature communications

Wang T,Wang D,Yu H,Feng B,Zhou F,Zhang H,Zhou L,Jiao S,Li Y

45-0031 was used in Flow cytometry/Cell sorting to assess the efficacy of a personalised cancer vaccine for eliciting postsurgical tumour specific-immunity.

Wed Apr 18 00:00:00 EDT 2018

Macrophages orchestrate breast cancer early dissemination and metastasis.

Nature communications

Linde N,Casanova-Acebes M,Sosa MS,Mortha A,Rahman A,Farias E,Harper K,Tardio E,Reyes Torres I,Jones J,Condeelis J,Merad M,Aguirre-Ghiso JA

45-0031 was used in Flow cytometry/Cell sorting to show that, in pre-malignant lesions, CCL2 produced by cancer cells and myeloid cells attracts CD206+/Tie2+macrophages and induces Wnt-1 upregulation that in turn downregulates E-cadherin junctions in the HER2+early cancer cells.

Tue Jan 02 00:00:00 EST 2018

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

45-0031 was used in Flow cytometry/Cell sorting to define a fundamental function of Egr2 and Egr3, operating as a checkpoint controlling the transition between clonal expansion and differentiation of effector T cells.

Mon Jun 05 00:00:00 EDT 2017

Differential cytokine contributions of perivascular haematopoietic stem cell niches.

Nature cell biology

Asada N,Kunisaki Y,Pierce H,Wang Z,Fernandez NF,Birbrair A,Ma'ayan A,Frenette PS

45-0031 was used in Flow cytometry/Cell sorting to demonstrate that the effects of cytokines regulating haematopoietic stem cell functions are dependent on the producing cell sources.

Wed Mar 01 00:00:00 EST 2017

Oncogenic mTOR signalling recruits myeloid-derived suppressor cells to promote tumour initiation.

Nature cell biology

Welte T,Kim IS,Tian L,Gao X,Wang H,Li J,Holdman XB,Herschkowitz JI,Pond A,Xie G,Kurley S,Nguyen T,Liao L,Dobrolecki LE,Pang L,Mo Q,Edwards DP,Huang S,Xin L,Xu J,Li Y,Lewis MT,Wang T,Westbrook TF,Rosen JM,Zhang XH

45003182 was used in flow cytometry to describe the role of mTOR signalling in recruiting pro-tumorigenic myeloid-derived suppressor cells.

Wed Jun 01 00:00:00 EDT 2016

FAK regulates platelet extravasation and tumor growth after antiangiogenic therapy withdrawal.

The Journal of clinical investigation

Haemmerle M,Bottsford-Miller J,Pradeep S,Taylor ML,Choi HJ,Hansen JM,Dalton HJ,Stone RL,Cho MS,Nick AM,Nagaraja AS,Gutschner T,Gharpure KM,Mangala LS,Rupaimoole R,Han HD,Zand B,Armaiz-Pena GN,Wu SY,Pecot CV,Burns AR,Lopez-Berestein G,Afshar-Kharghan V,Sood AK

45-0031 was used in Flow cytometry/Cell sorting to identify focal adhesion kinase, FAK, as a target for ovarian cancer management in situations in which antiangiogenic agents are withdrawn.

Mon May 02 00:00:00 EDT 2016

A critical role for cellular inhibitor of protein 2 (cIAP2) in colitis-associated colorectal cancer and intestinal homeostasis mediated by the inflammasome and survival pathways.

Mucosal immunology

Dagenais M,Dupaul-Chicoine J,Champagne C,Skeldon A,Morizot A,Saleh M

45-0031 was used in Immunofluorescence-paraffin to investigate the function of cellular inhibitors of apoptosis proteins in intestinal cancer and mechanisms in disease pathogenesis.

Fri Jan 01 00:00:00 EST 2016

Caspase-12, but Not Caspase-11, Inhibits Obesity and Insulin Resistance.

Journal of immunology (Baltimore, Md. : 1950)

Skeldon AM,Morizot A,Douglas T,Santoro N,Kursawe R,Kozlitina J,Caprio S,Mehal WZ,Saleh M

45-0031 was used in Flow cytometry/Cell sorting to investigate a role for caspase-11 and caspase-12 in obesity and insulin resistance.

Fri Jan 01 00:00:00 EST 2016

Macrophage autophagy limits acute toxic liver injury in mice through down regulation of interleukin-1β.

Journal of hepatology

Ilyas G,Zhao E,Liu K,Lin Y,Tesfa L,Tanaka KE,Czaja MJ

45-0031 was used in Flow cytometry/Cell sorting to show that macrophage autophagy functions to limit acute toxin-induced liver injury and death by inhibiting the generation of inflammasome-dependent IL-1β.

Fri Jan 01 00:00:00 EST 2016

HSP70 vaccine in combination with gene therapy with plasmid DNA encoding sPD-1 overcomes immune resistance and suppresses the progression of pulmonary metastatic melanoma.

International journal of cancer

Geng H,Zhang GM,Xiao H,Yuan Y,Li D,Zhang H,Qiu H,He YF,Feng ZH

Thu Jun 01 00:00:00 EDT 2006

Functional analysis of B and T lymphocyte attenuator engagement on CD4+ and CD8+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Krieg C,Han P,Stone R,Goularte OD,Kaye J

Tue Nov 15 00:00:00 EST 2005

CCR5-deficient mice develop experimental autoimmune uveoretinitis in the context of a deviant effector response.

Investigative ophthalmology & visual science

Takeuchi A,Usui Y,Takeuchi M,Hattori T,Kezuka T,Suzuki J,Okunuki Y,Iwasaki T,Haino M,Matsushima K,Usui M

Sat Oct 01 00:00:00 EDT 2005

生物信息学

蛋白别名: CD247; CD247 antigen; CD3 antigen, zeta polypeptide; CD3 TCR complex; CD3-zeta/eta; CD3d; CD3e; CD3g; FLJ18683; Leu-4; T cell antigen receptor complex epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell receptor T3 eta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex

基因别名: 4930549J05Rik; A430104F18Rik; AI504783; AW552088; Cd247; CD3; Cd3-eta; Cd3-zeta; Cd3d; Cd3e; CD3epsilon; Cd3g; Cd3h; Cd3z; Cd3zeta; Ctg-3; Ctg3; T3d; T3e; T3g; T3z; Tcrk; Tcrz

UniProt ID: (Mouse) P24161, (Mouse) P04235, (Mouse) P22646, (Mouse) P11942

Entrez Gene ID: (Mouse) 12503, (Mouse) 12500, (Mouse) 12501, (Mouse) 12502

分子生物学功能: defense/immunity protein immunoglobulin receptor superfamily

功能

参与通路

transmembrane signaling receptor activity protein binding identical protein binding protein heterodimerization activity signal transducer activity SH3 domain binding

CD3e Monoclonal Antibody (145-2C11), PerCP-Cyanine5.5, eBioscience™

产品信息

45-0031-82

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

流式细胞分析 (Flow)

功能检测 (FN)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

随时为您服务

Brands

Shopping Tools

eSolutions

Support

我们的公司

Your items have has been added!