Invitrogen

CD40 Monoclonal Antibody (LOB7/6)

Name: CD40 Monoclonal Antibody (LOB7\/6)
Brand: Invitrogen
SKU: MA1-80926

1 篇参考文献

查看其他130个CD40抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)

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CD40 Antibody (MA1-80926) in Flow

Flow cytometric analysis of human peripheral blood lymphocytes using a CD40/Bp50 monoclonal antibody (Product # MA1-80926)

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产品信息

MA1-80926

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1-10 µg/mL

免疫组化（冰冻） (IHC (F))

1-10 µg/mL

流式细胞分析 (Flow)

20 µg/mL

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免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Dog, Human

已发表种属

C. elegans

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

LOB7/6

抗原

CD40 Ig(Fc) fusion protein containing the EC region of human CD40 and Fc region of human IgG

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS

内含物

<0.1% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_928288

产品详细信息

This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase. A suggested positive control for immunohistochemical applications is human tonsil. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Mouse anti Human CD40 antibody, clone LOB7/6 recognizes the human CD40 cell surface antigen, a 48kDa glycoprotein expressed by B lymphocytes and weakly by some monocytes.

靶标信息

CD40 is a single-chain glycoprotein and a member of the tumor necrosis factor receptor (TNFR) family, exhibiting significant homology to the Hodgkin's disease-associated antigen, CD30. It is expressed by B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells, as well as some epithelial cells, carcinomas, and lymphoid dendritic cells. Notably, CD40 is present on all B cells except plasma cells. CD40 plays a crucial role in regulating B cell development and maturation, inducing immunoglobulin isotype-switching, and protecting B cells from surface Ig-induced apoptosis when combined with other signals such as IL-4. It promotes proliferation and is essential for T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. The interaction between CD40 and its ligand CD154 (gp39) on T cells is vital for T-B cell crosstalk, costimulation, and immune regulation. Adaptor protein TNFR2 interacts with CD40, mediating signal transduction, while the AT-hook transcription factor AKNA is reported to regulate CD40 expression, which may be important for homotypic cell interactions. Additionally, the interaction between CD40 and its ligand is necessary for amyloid-beta-induced microglial activation, suggesting a role in the early pathogenesis of Alzheimer's disease. Two alternatively spliced transcript variants encoding distinct isoforms of CD40 have been identified. Diseases associated with CD40 dysfunction include Type 3 Hyper-IgM immunodeficiency and CD40 ligand deficiency, highlighting its importance in immune and inflammatory responses.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: B cell surface antigen CD40; B cell-associated molecule; B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40 antigen (TNF receptor superfamily member 5); CD40 molecule, TNF receptor superfamily member 5; CD40L receptor; CDw40; Immunoglobulin M; ImmunoglobulinM; MGC9013; receptor for ligand CD154; sCD40; soluble CD40; Tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

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基因别名: Bp50; CD40; CDW40; p50; TNFRSF5

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UniProt ID: (Dog) Q7YRL5, (Human) P25942

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Entrez Gene ID: (Dog) 403469, (Human) 958

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功能

signaling receptor activity antigen binding signal transducer activity receptor activity tumor necrosis factor-activated receptor activity protein binding enzyme binding ubiquitin protein ligase binding transmembrane signal receptor

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