Invitrogen

CD41 Polyclonal Antibody

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CD41 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CD41 was performed on equine endometrial tissue sections. 5-µm-thick sections were prepared from formalin-fixed paraffin-embedded tissue blocks and were dried on slide plate at 37°C overnight. The sections were placed in a Bond Max Automated Immunohistochemistry Vision Biosystem (Leica Microsystems GmbH, Wetzlar, Germany) according to the following protocol. First, tissues were deparaffinized and pre-treated with Epitope Retrieval Solution 1 (Citrate based pH6) at 98°C for 10 minutes. After washing steps, peroxidase blocking was carried out for 10 min using the Bond Polymer Refine Detection Kit DC9800 (Leica Microsystems GmbH). Tissues were again washed and then incubated with the primary antibody (Product # PA5-22307, 1:200) for 30 min then the secondary antibody diluted 1:1000. Subsequently, tissues were incubated with polymer for 10 min and developed with DAB-Chromogen for 10 min. Data courtesy of Antibody Data Exchange Program.

CD41 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of Human Platelets (Lane 1). The blot was probed with CD41 Polyclonal Antibody (Product # PA5-22307, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 125 kDa band corresponding to CD41 was detected in the lysate tested.

产品信息

PA5-22307

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:3,000	-
免疫组化（石蜡） (IHC (P))	1:100-1:1,000	-
免疫细胞化学 (ICC/IF)	Assay-dependent	-

产品规格
种属反应	Human, Mouse
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant fragment corresponding to a region within amino acids 210 and 532 of Integrin alpha 2b (Uniprot ID#P08514)
偶联物	Unconjugated
形式	Liquid
浓度	0.25 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7, with 20% glycerol, 1% BSA
内含物	0.025% ProClin 300
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_11155042

产品详细信息

Recommended positive controls: A549, mouse spleen.

Predicted reactivity: Mouse (89%), Dog (88%), Pig (82%), Rabbit (87%), Bovine (86%).

PA5-22307 detects ITGA2b (CD41) protein in formalin-fixed paraffin-embedded equine endometrial tissue.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

CD41 (platelet glycoprotein IIb, ITGA2B) is composed of two subunits -120 kDa a, alpha and 23 kDa b, beta- that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. CD41 is also involved in blood coagulation by mediating platelet aggregation. Upon blood vessel damage, CD41 binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during the early stages of hematopoietic differentiation. Diseases associated with CD41 dysfunction include Glansmann Thrombasthenia, and Platelet type-16 bleeding disorder.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: alpha IIb; alphaIIb protein; CD41; form 1; form 2; GP IIb; GPalpha IIb; GPIIb; Integrin alpha-IIb; Integrin alpha-IIb heavy chain; Integrin alpha-IIb light chain; Integrin alpha-IIb light chain, form 1; Integrin alpha-IIb light chain, form 2; integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41); platelet fibrinogen receptor, alpha subunit; platelet glycoprotein IIb of IIb/II Ia complex; platelet glycoprotein IIb of IIb/IIIa complex; Platelet membrane glycoprotein IIb; platelet-specific antigen BAK; protein phosphatase 1, regulatory subunit 93

基因别名: AI172977; alphaIIb; BDPLT16; BDPLT2; CD41; CD41B; GP2B; GPIIb; GT; GTA; HPA3; ITGA2B; ITGAB; PPP1R93

UniProt ID: (Human) P08514, (Mouse) Q9QUM0

Entrez Gene ID: (Human) 3674, (Mouse) 16399

分子生物学功能: integrin