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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD44 Antibodies

          Invitrogen

          CD44 Monoclonal Antibody (156-3C11)

          高级验证
          20个已发表图片
          82 篇参考文献
          查看其他176个CD44抗体

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          说明书
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          说明书
          实验方案
          问题与答案
          技术支持

          Cite CD44 Monoclonal Antibody (156-3C11)

          • 抗体检测数据 (17)
          • 已发表图片 (20)
          • 高级验证 (4)
          CD44 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          CD44 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 41

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          CD44 Antibody (MA5-13890) in ICC/IF

          Immunofluorescence of CD44 was performed using HAP1 wild-type and CD44 KO cells that were transfected with a green or a far red fluorescent dye, respectively. Post-transfection, WT and KO cells were mixed and plated to a 1:1 ratio on coverslips as a mosaic and incubated for 24 hrs. Cells were fixed in 4% PFA (in PBS) for 15 min; cells were permeabilized with 0.1% Triton X-100 for 10 min at RT and blocked with PBS with 5% BSA, 5% goat serum, and 0.01% Triton X-100 for 30 min. Cells were stained with the CD44 monoclonal antibody (Product # M... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          CD44 Antibody in Immunocytochemistry (ICC/IF)
          CD44 Antibody in Immunocytochemistry (ICC/IF)
          CD44 Antibody in Immunocytochemistry (ICC/IF)
          CD44 Antibody in Immunocytochemistry (ICC/IF)
          CD44 Antibody in Immunocytochemistry (ICC/IF)
          CD44 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD44 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD44 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD44 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD44 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD44 Antibody in Western Blot (WB)
          CD44 Antibody in Western Blot (WB)
          CD44 Antibody in Western Blot (WB)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Immunoprecipitation (IP)
          CD44 Antibody in Immunoprecipitation (IP)
          CD44 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD44 Antibody in Immunocytochemistry (ICC/IF)
          CD44 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          CD44 Antibody in Immunohistochemistry (IHC)
          CD44 Antibody in Immunohistochemistry (IHC)
          CD44 Antibody in Immunohistochemistry (IHC)
          CD44 Antibody in Immunohistochemistry (IHC)
          CD44 Antibody in Immunohistochemistry (IHC)
          CD44 Antibody in Immunohistochemistry (IHC)
          CD44 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          CD44 Antibody in Western Blot, Immunohistochemistry (WB, IHC)
          CD44 Antibody in Immunohistochemistry (IHC)
          CD44 Antibody in Immunohistochemistry (IHC)
          CD44 Antibody in Western Blot (WB)
          CD44 Antibody in Western Blot (WB)
          CD44 Antibody in Western Blot (WB)
          CD44 Antibody in Western Blot (WB)
          CD44 Antibody in Western Blot (WB)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody in Flow Cytometry (Flow)
          CD44 Antibody
          CD44 Antibody
          CD44 Antibody
          CD44 Antibody

          产品信息

          MA5-13890

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1-2 µg/mL
          查看7篇已发表文章 7篇已发表文章

          免疫组化 (IHC)

          -
          查看48篇已发表文章 48篇已发表文章

          免疫组化(石蜡) (IHC (P))

          1-2 µg/mL
          查看8篇已发表文章 8篇已发表文章

          免疫细胞化学 (ICC/IF)

          1:200
          查看6篇已发表文章 6篇已发表文章

          流式细胞分析 (Flow)

          2 µg/test
          查看7篇已发表文章 7篇已发表文章

          免疫沉淀 (IP)

          2 µg/mL
          -

          in situ PLA (PLA)

          -
          查看1篇已发表文章 1篇已发表文章

          抑制试剂 (IA)

          -
          查看1篇已发表文章 1篇已发表文章

          阻断反应 (BLOCK)

          -
          查看1篇已发表文章 1篇已发表文章

          其他PubMed (Misc)

          -
          查看3篇已发表文章 3篇已发表文章
          产品规格

          种属反应

          Baboon, Human, Non-human primate

          已发表种属

          Human, Rabbit, Rhesus monkey

          宿主/亚型

          Mouse / IgG2a, kappa

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          156-3C11

          抗原

          Stimulated human leukocytes
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.2 mg/mL

          纯化类型

          Protein A/G

          保存液

          PBS, pH 7.4, with 0.05% BSA

          内含物

          0.05% sodium azide

          保存条件

          4°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10986810

          产品详细信息

          MA5-13890 targets CD44 in FACS, IHC (P), ICC/IF, IP and WB applications and shows reactivity with Human, Baboon and Non-human primate samples.

          The MA5-13890 immunogen is stimulated human leukocytes.

          MA5-13890 was used in the immunofluorescence analysis to successfully detect CD44 in human mesenchymal stem cells derived from iPSCs.

          靶标信息

          CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: CD44; CD44 antigen; CD44 molecule; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; ECMRIII; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; PGP I; PGP-1; PGP-I; PGPI; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; sCD 44; sCD44; soluble CD 44; soluble CD44

          查看更多 收起

          基因别名: CD44; CDW44; CSPG8; ECMR-III; HCELL; HUTCH-I; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1

          查看更多 收起

          UniProt ID: (Human) P16070

          查看更多 收起

          Entrez Gene ID: (Human) 960

          查看更多 收起

          功能
          cytokine receptor activity protein binding collagen binding hyaluronic acid binding transmembrane signal receptor
          参与通路
          cell-matrix adhesion single organismal cell-cell adhesion extracellular matrix disassembly extracellular matrix organization hyaluronan catabolic process positive regulation of peptidyl-serine phosphorylation positive regulation of heterotypic cell-cell adhesion negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator cellular response to fibroblast growth factor stimulus positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration cartilage development interferon-gamma-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade monocyte aggregation positive regulation of monocyte aggregation negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
          It has to be done as per old AB suggested Products section.
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          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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