Invitrogen

CD45 Monoclonal Antibody (HI30), Alexa Fluor™ 647, eBioscience™

Product Image Gallery

CD45 Antibody in Flow Cytometry (Flow) — Normal human peripheral blood cells were stained with Mouse IgG1 kappa Isotype Control, Alexa Fluor 647 (Product # 51-4714-81) (blue histogram) or CD45 Monoclonal Antibody, Alexa Fluor 647 (purple histogram). Cells in the lymphocyte gate were used for analysis.

CD45 Antibody in Immunocytochemistry (ICC/IF) — Figure 2 Separation of CTCs and leukocyte or endothelial cells. ( A ) Technical concept using CTC-FIND for single cell isolation. ( B ) Scheme of cell classification. ( C - E ) Representative fluorescence image of CTCs detected in clinical blood specimens. The blood samples (8 mL) collected from patients with mCRC were evaluated by fluorescence microscopy after separation to single cells by CTC-FIND. Specimens were enriched and stained with Hoechst 33342 (pseudo colored blue) and negative markers composed of anti-CD45 and anit-CD50 antibodies (pseudo colored red), followed by the acquisition of fluorescence images for each marker and digital overlay of the two images (merge). Hoechst 33342-positive and CD45/50-negative CTCs were counted and collected from each specimen. Gallery of CTCs ( C ), CTC clusters ( D ) and leucocytes ( E ). Original magnification, x500.

CD45 Antibody in Immunohistochemistry (IHC) — Fig. 4 Silence of Usp47 inhibits BCR-ABL T315I -induced CML and inhibits proliferation of KBM T315I in a xenograft model. a Survival of mice after the transplantation of BCR-ABL T315I -transduced Usp47 +/+ or Usp47 -/- BM cells ( n = 6 biologically independent samples per group). **** p < 0.0001, p -value was analyzed by Mantel-Cox-log-rank test. The experiment was repeated three times. b , c Size ( b ) and weight ( c ) of the spleen in the two groups of mice ( n = 5 biologically independent samples per group) at 28 days after transplantation. Center lines of the box and whisker plot represent median values, whereas the box edges indicate the 25th and 75th centiles, and the whiskers indicate the minimum and maximum values. d , e At 28 days after transplantation, the morphology of cells from PB ( d ) was examined by Wright-Giemsa staining; the percentages of GFP + and Gr-1 + cells ( e ) in BM were examined by FACS analysis. f H&E staining of the liver and spleen at 28 days after transplantation. Scale bars, 50 mum. g Ctrl shRNA or ShUSP47-2 transfected KBM5 T315I cells ( n = 8 biologically independent samples per group) were injected into B-NDG mice through the tail vein. **** p < 0.0001, p- value was analyzed by Mantel-Cox-log-rank test. h Size and weight of spleen at day 21 ( n = 6 biologically independent samples per group). Center lines of the box and whisker plot represent median values, whereas the box edges indicate the 25th and 75th centiles, and the whiskers indicate

产品信息

51-0459-42

应用	建议稀释比	已发表文章
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
免疫细胞化学 (ICC/IF)	-	查看1篇已发表文章 1篇已发表文章
流式细胞分析 (Flow)	5 µL (0.125 µg)/test	查看12篇已发表文章 12篇已发表文章

产品规格
种属反应	Human
已发表种属	Not Applicable
宿主/亚型	Mouse / IgG1, kappa
推荐的同型对照	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 647, eBioscience™
分类	Monoclonal
类型	Antibody
克隆号	HI30
偶联物	Alexa Fluor™ 647 Alexa Fluor 532 Alexa Fluor 561 Alexa Fluor 660 APC APC-eFluor 780 Biotin Brilliant UV 395 Brilliant UV 496 Brilliant UV 563 Brilliant UV 737 Brilliant UV 805 Brilliant Violet 421 Brilliant Violet 480 Brilliant Violet 650 Brilliant Violet 711 eFluor 450 eFluor 506 FITC NovaFluor Blue 510 NovaFluor Blue 585 NovaFluor Blue 690 NovaFluor Red 710 NovaFluor Red 725 NovaFluor Red 755 N
激发/发射光谱	650/671 nm 查看光谱
形式	Liquid
浓度	5 µL/Test
纯化类型	Affinity chromatography
保存液	PBS, pH 7.2, with 0.2% BSA
内含物	0.09% sodium azide
保存条件	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2802301

产品详细信息

Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This HI30 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser

靶标信息

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

基因别名: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

UniProt ID: (Human) P08575

Entrez Gene ID: (Human) 5788

分子生物学功能: protein phosphatase protein modifying enzyme

功能

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

参与通路

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding

CD45 Monoclonal Antibody (HI30), Alexa Fluor™ 647, eBioscience™

产品信息

51-0459-42

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

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生物信息学

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