Invitrogen

CD45RA Monoclonal Antibody (MEM-56), PE-Cyanine7

CD45RA Antibody in Flow Cytometry (Flow)

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CD45RA Antibody (MA562323) in Flow

Flow cytometry (surface staining) of CD45RA in human peripheral blood. Samples were stained with CD45RA monoclonal antibody (Product # MA562323).

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产品信息

MA562323

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

4 µL/100 µL whole blood or 10^6 cells

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

MEM-56

抗原

Human thymocytes and T lymphocytes.

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偶联物

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

激发/发射光谱

569/780 nm 查看光谱

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Size-exclusion chromatography

保存液

PBS, pH 7.4, with 0.2% BSA

内含物

15mM sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing and these isoforms differ in their extracellular domains, however, they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 is expressed on cells of the human hematopoietic lineage, with the exception of mature red cells and is not detected on differentiated cells of other tissues. It is likely that CD45 plays an important role in signal transduction, inhibition or upregulation of various immunological functions. Diseases associated with CD45 dysfunction include severe combined immunodeficiency and Hepatitis C.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD45; CD45 antigen; L-CA; Leukocyte common antigen; Ly-5; Lyt-4; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T100; T200; T200 glycoprotein; T200 leukocyte common antigen

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基因别名: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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功能

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

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