For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
CD47 (integrin-associated protein, IAP) is a ubiquitously expressed cell surface transmembrane glycoprotein interacting with several integrins and regulating their functions. Engagement of CD47 by soluble ligands or counter receptors modulates various signaling pathways, such as activation of heterotrimeric G proteins. Functionally, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. CD47 is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. CD47 has broad tissue distribution, is reduced in expression on Rh erythrocytes. CD47 acts as a ligand for CD172a (signal regulatory protein alpha, SIRP alpha), an immune inhibitory receptor on macrophages; this interaction prevents phagocytosis of CD47-positive cells. Moreover, CD47-CD172a system affects cell migration, B cell adhesion, T cell activation, and is involved in modulation of chondrocyte responses to mechanical signals, and promotes neuronal development, being especially abundant in synapse-rich regions of brain and retina.
蛋白别名: CD47; CD47 antigen; CD47 antigen (Rh-related antigen, integrin-associated signal transducer); IAP; Integrin-associated protein; Leukocyte surface antigen CD47; sCD47; soluble CD 47; soluble CD47
基因别名: Cd47; Itgp
UniProt ID: (Rat) P97829
Entrez Gene ID: (Rat) 29364
分子生物学功能: cell adhesion molecule