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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • CD49f (Integrin alpha 6) Antibodies

          Invitrogen

          CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), APC, eBioscience™

          54个已发表图片
          56 篇参考文献
          查看其他36个CD49f (Integrin alpha 6)抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), APC, eBioscience™

          • 抗体检测数据 (1)
          • 已发表图片 (54)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          图: 1 / 55

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          CD49f (Integrin alpha 6) Antibody (17-0495-82) in Flow

          Staining of normal human peripheral blood cells with 0.06 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (blue histogram) or 0.06 µg of Anti-Human/Mouse CD49f (Integrin alpha 6) APC (purple histogram). Cells in the lymphocyte gate were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)
          CD49f (Integrin alpha 6) Antibody in Flow Cytometry (Flow)

          产品信息

          17-0495-82

          应用
          建议稀释比
          已发表文章

          流式细胞分析 (Flow)

          0.125 µg/test
          查看55篇已发表文章 55篇已发表文章

          其他PubMed (Misc)

          -
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Human, Mouse

          已发表种属

          Human, Mouse

          宿主/亚型

          Rat / IgG2a, kappa

          推荐的同型对照

          Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          eBioGoH3 (GoH3)

          偶联物

          APC APC APC
          • Unconjugated
          • Alexa Fluor 700 
          • APC-eFluor 780
          • Biotin 
          • eFluor 450
          • FITC
          • PE 
          • PE-Cyanine7
          • PerCP-eFluor 710
          • Super Bright 436
          • Super Bright 600
          • Super Bright 780
          • Request custom conjugation

          激发/发射光谱

          651/660 nm 查看光谱 spectra

          形式

          Liquid

          浓度

          0.2 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2

          内含物

          0.09% sodium azide

          保存条件

          4°C, store in dark, DO NOT FREEZE!

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2016694

          产品详细信息

          Description: The eBioGoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6 (VLA-6 alpha chain), and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex; CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The eBioGoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin.

          Applications Reported: This eBioGoH3 (GoH3) antibody has been reported for use in flow cytometric analysis.

          Applications Tested: This eBioGoH3 (GoH3) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          靶标信息

          CDf (Integrin alpha-6, ITGA6) is the integrin alpha chain alpha 6 protein. Integrins are integral cell-surface proteins composed of an alpha chain and a beta chain. A given chain may combine with multiple partners resulting in different integrins. For example, alpha 6 may combine with beta 4 in the integrin referred to as TSP180, or with beta 1 in the integrin VLA-6. Integrins are known to participate in cell adhesion as well as cell-surface mediated signaling. Two transcript variants encoding different isoforms have been found for CDf. Functionally, integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals that affect cellular shape, mobility, and progression through the cell cycle in response to the extracellular matrix. Integrin receptors are composed of alpha and beta subunits, and form structural and functional linkages between the ECM (extra-cellular matrix) and intracellular cytoskeletal linker proteins. Signaling mediated by Intergrin/ECM interactions are also integrated with cellular responses to growth factor signaling to regulate cellular proliferation, cytoskeletal reorganization and other responses necessary for cellular survival. CDf is predominantly expressed by epithelia, and plays a critical structural role in the hemidesmosome. More than 18 alpha and 8 beta subunits with numerous splice variant isoforms have been identified in mammals. Certain integrins can also bind to soluble ligands or to counter-receptors on adjacent cells, such as the intracellular adhesion molecules (ICAMs), resulting in aggregation of cells.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          生物信息学

          蛋白别名: CD49 antigen-like family member F; CD49f; DKFZp686J01244; FLJ18737; integrin alpha 6; Integrin alpha-6; integrin alpha6B; integrin, alpha 6; VLA-6

          查看更多 收起

          基因别名: 5033401O05Rik; AI115430; CD49f; ITGA6; ITGA6B; VLA-6

          查看更多 收起

          UniProt ID: (Human) P23229, (Mouse) Q61739

          查看更多 收起

          Entrez Gene ID: (Human) 3655, (Mouse) 16403

          查看更多 收起

          功能
          integrin binding protein binding insulin-like growth factor I binding neuregulin binding metal ion binding cadherin binding involved in cell-cell adhesion protein complex binding laminin binding integrin
          参与通路
          cell-substrate junction assembly cell-matrix adhesion integrin-mediated signaling pathway ectodermal cell differentiation positive regulation of cell-cell adhesion extracellular matrix organization hemidesmosome assembly cell-substrate adhesion cellular response to extracellular stimulus cell adhesion mediated by integrin nail development positive regulation of phosphorylation positive regulation of apoptotic process positive regulation of GTPase activity skin development positive regulation of transcription from RNA polymerase II promoter filopodium assembly digestive tract development brown fat cell differentiation leukocyte migration renal system development amelogenesis cell-cell adhesion negative regulation of extrinsic apoptotic signaling pathway cell adhesion positive regulation of cell-substrate adhesion single organismal cell-cell adhesion odontogenesis of dentin-containing tooth
          It has to be done as per old AB suggested Products section.
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          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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