Invitrogen

CD50 (ICAM-3) Monoclonal Antibody (CBR-IC3/1), FITC, eBioscience™

CD50 (ICAM-3) Antibody in Flow Cytometry (Flow)

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CD50 (ICAM-3) Antibody (BMS111FI) in Flow

Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD41 FITC and CD50 FITC. As expected based on known relative expression patterns, CD50 clone CBR-IC3/1 stains lymphocytes (pink), monocytes (orange), granulocytes (blue) and a small subset of CD45 negative cells (green).

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产品信息

BMS111FI

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

CBR-IC3/1

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

10 µL/Test

纯化类型

Affinity chromatography

保存液

TBS, pH 7.2-7.4, with 1% BSA

内含物

0.02% sodium azide

保存条件

4°C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_10597745

产品详细信息

Description: Intercellular Adhesion Molecule-3 (ICAM-3) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1). ICAM-3 is a heavily glycosylated, integral membrane protein with five immunoglobulin-like domains. It shares high homology to ICAM-1 and ICAM-2 in the extracellular region. In contrast to ICAM-1 and ICAM-2, ICAM-3 is absent on endothelia. ICAM-3 is expressed on resting lymphocytes, monocytes and neutrophils, representing the major LFA-1 ligand on these cells. The finding that adhesion of resting T lymphocytes to LFA-1 occurs primarily via ICAM-3 combined with the fact that ICAM-3 is much better expressed than other LFA-1 ligands on monocytes and resting lymphocytes implies an important role for ICAM-3 in the initiation of immune responses.

Applications Tested: Flow Cytometry.

Purity: >95%.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser

靶标信息

CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。