Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (1A29), PerCP-eFluor™ 710, eBioscience™

Name: CD54 (ICAM-1) Monoclonal Antibody (1A29), PerCP-eFluor\u2122 710, eBioscience\u2122
Brand: Invitrogen
SKU: 46-0540-80

1 篇参考文献

查看其他114个ICAM-1 (CD54)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)

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CD54 (ICAM-1) Antibody (46-0540-80) in Flow

Staining of rat splenocytes with 0.06 µg of Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or 0.06 µg of Anti-Rat CD54 (ICAM-1) PerCP-eFluor® 710 (purple histogram). Cells in the lymphocyte gate were used for analysis.

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产品信息

46-0540-80

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

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产品规格

种属反应

Rat

已发表种属

Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

1A29

偶联物

PerCP-eFluor™ 710 PerCP-eFluor™ 710 PerCP-eFluor™ 710

激发/发射光谱

482/708 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_10854127

产品详细信息

Description: The 1A29 monoclonal antibody reacts with rat CD54, which is also known as intercellular adhesion molecule 1 (ICAM-1). In the immune system, this type I immunoglobulin (Ig)-like transmembrane protein is expressed on lymphocytes, macrophages, dendritic cells, monocytes, granulocytes, and thymic stromal cells. CD54 is also present on endothelial cells and such non-immune cells as hepatocytes, cardiomyocytes, and vascular smooth muscle cells. In addition, reports demonstrate that CD54 expression can be induced in vitro by proinflammatory cytokines, including IFN-gamma, IL-1, CCL2, or TNF-alpha. CD54 plays important roles in transendothelial migration of leukocytes to sites of inflammation and in T cell interactions with antigen presenting cells. Studies indicate that CD54 mediates these functions by binding to lymphocyte function-associated molecule (LFA-1; also known as CD11a/CD18).

The 1A29 antibody has been reported to block interactions between CD54 and LFA-1.

Applications Reported: This 1A29 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 1A29 antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells

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基因别名: CD54; ICAM; Icam-1; Icam1

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UniProt ID: (Rat) Q00238

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Entrez Gene ID: (Rat) 25464

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功能

integrin binding protein complex binding

参与通路