Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (R6.5), eBioscience™

产品信息

BMS1011

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

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酶联免疫吸附实验 (ELISA)

Assay-Dependent

抑制试剂 (IA)

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

R6.5

抗原

Rec. Human ICAM-1

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2-7.4

内含物

no preservative

保存条件

-20°C

运输条件

Wet ice

RRID

AB_10596660

产品详细信息

Description: Anti-ICAM-1 Monoclonal Antibody R6.5, a murine IgG2a mAb to the human ICAM-1, inhibits leukocyte adhesion to the vascular endothelium, thereby decreasing leukocyte extravasation and inflammatory tissue injury.

Intercellular Adhesion Molecule-1 (ICAM-1) is a member of the immunoglobulin supergene family and functions as a ligand for the Lymphocyte Function-Associated Antigen-1 (LFA-1), an alpha-betacomplex that is a member of the leukocyte integrin family of cell-cell and cell-matrix receptors.

ICAM-1 is a single-chain glycoprotein with a polypeptide core of 55 kD that can be expressed on non-hematopoietic cells of many lineages such as vascular endothelial cells, thymic epithelial cells, other epithelial cells and fibroblasts and on hematopoietic cells such as tissue macrophages, mitogen-stimulated T-lymphoblasts, germinal center B cells and dendritic cells in tonsils, lymph nodes and Peyer's patches.

ICAM-1 is inducible on fibroblasts and endothelial cells by inflammatory mediators such as IL-1, TNF and IFN-gamma within few hours and is correlated to the infiltration of lymphocytes into inflammatory lesions. ICAM-1 seems to be the initial marker of inflammatory reactions and is expressed prior to, and to a greater extent than is HLA-DR.

sICAM-1 serum levels were found elevated in acute renal graft rejection, insulin-dependent diabetis mellitus, anterior uveitis and in allergic inflammation.

Applications Tested: ELISA.

靶标信息

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Surface Modifications for Improved Delivery and Function of Therapeutic Bacteria.

Small (Weinheim an der Bergstrasse, Germany)

Vargason AM,Santhosh S,Anselmo AC

BMS1011 was used in Immunocytochemistry-immunoflourescence to demonstrate the first application of traditional pharmacokinetic analysis to design and evaluate LTB drug delivery systems and provides a platform toward controlling adhesion, colonization, and efficacy of LTBs.

Mon Jun 01 00:00:00 EDT 2020

ICAM-1 Binding Rhinoviruses Enter HeLa Cells via Multiple Pathways and Travel to Distinct Intracellular Compartments for Uncoating.

Viruses

Ganjian H,Zietz C,Mechtcheriakova D,Blaas D,Fuchs R

BMS1011 was used in Immunocytochemistry-immunoflourescence to demonstrate that RV-B14 and RV-A89 enter via clathrin-, dynamin-, and cholesterol-dependent pathways, as well as by macropinocytosis.

Sat Apr 01 00:00:00 EDT 2017

Phenotyping of Leukocytes and Leukocyte-Derived Extracellular Vesicles.

Journal of immunology research

Pugholm LH,Bæk R,Søndergaard EK,Revenfeld AL,Jørgensen MM,Varming K

BMS1011 was used in Antibody array to investigate the expression of selected immunological lineage-specific markers and selected vesicle-related markers on five major leukocyte subpopulations.

Mon Mar 06 00:00:00 EST 2017

The impact of various preanalytical treatments on the phenotype of small extracellular vesicles in blood analyzed by protein microarray.

Journal of immunological methods

Bæk R,Søndergaard EK,Varming K,Jørgensen MM

BMS1011 was used to optimize the collection and analysis of extracellular vesicles

Tue Nov 01 00:00:00 EDT 2016

Characterization of a Cell-Culturing System for the Study of Contact-Independent Extracellular Vesicle Communication.

Journal of circulating biomarkers

Revenfeld ALS,Søndergaard EKL,Stensballe A,Bæk R,Jørgensen MM,Varming K

BMS1011 was used in Antibodies/proteins for the phenotyping of vesicles to characterise an experimental system to study dynamic vesicle based cell communication.

Thu Oct 01 00:00:00 EDT 2020

Neutrophil recruitment limited by high-affinity bent β2 integrin binding ligand in cis.

Nature communications

Fan Z,McArdle S,Marki A,Mikulski Z,Gutierrez E,Engelhardt B,Deutsch U,Ginsberg M,Groisman A,Ley K

BMS1011 was used in Inhibition experiments to show that a substantial fraction of Β2 integrins on human neutrophils acquire an unexpected E(-)H(+) conformation.

Wed Aug 31 00:00:00 EDT 2016

Potentials and capabilities of the Extracellular Vesicle (EV) Array.

Journal of extracellular vesicles

Jørgensen MM,Bæk R,Varming K

BMS1011 was used in Antibody array to develop methods for enrichment and purification of extracellular vesicles and exosomes from biofluids.

Sat Apr 11 00:00:00 EDT 2015

生物信息学

蛋白别名: CD54; cell surface glycoprotein P3.58; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; Ly 47; Major group rhinovirus receptor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells

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基因别名: BB2; CD54; ICAM1; P3.58

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UniProt ID: (Human) Q96B50

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Entrez Gene ID: (Human) 3383

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功能

virus receptor activity receptor activity transmembrane signaling receptor activity integrin binding protein binding

参与通路