Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), PE, eBioscience™

CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)

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CD54 (ICAM-1) Antibody (12-0541-81) in Flow

Staining of C57BL/6 splenocytes with 0.06 µg of Rat IgG2b kappa Isotype Control PE (Product # 12-4031-82) (open histogram) or 0.06 µg of Anti-Mouse CD54 (ICAM-1) PE (filled histogram). Total viable cells were used for analysis.

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产品信息

12-0541-81

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.125 µg/test

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产品规格

种属反应

Mouse

已发表种属

Mouse

宿主/亚型

Rat / IgG2b, kappa

分类

Monoclonal

类型

Antibody

克隆号

YN1/1.7.4

偶联物

PE PE PE

激发/发射光谱

565/576 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_465706

产品详细信息

Description: The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody.

Applications Reported: The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Product References

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12-0541-81 was used in Flow cytometry/Cell sorting to indicate that the IL6Myc model is useful for studying development of aggressive MM and for developing new treatments against such forms of the disease.

Fri Dec 01 00:00:00 UTC 2023

A Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Assay Identifies Nilotinib as an Inhibitor of Inflammation in Acute Myeloid Leukemia.

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Marín-Rubio JL,Peltier-Heap RE,Dueñas ME,Heunis T,Dannoura A,Inns J,Scott J,Simpson AJ,Blair HJ,Heidenreich O,Allan JM,Watt JE,Martin MP,Saxty B,Trost M

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Thu Sep 22 00:00:00 UTC 2022

Innate Immune Dysfunction in Rosacea Promotes Photosensitivity and Vascular Adhesion Molecule Expression.

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Kulkarni NN,Takahashi T,Sanford JA,Tong Y,Gombart AF,Hinds B,Cheng JY,Gallo RL

12-0541 was used in Immunohistochemistry to show how the expression of LL-37 can lead to enhanced sensitivity to UVB radiation in rosacea.

Sun Mar 01 00:00:00 UTC 2020

Regulatory T cells mediate specific suppression by depleting peptide-MHC class II from dendritic cells.

Nature immunology

Akkaya B,Oya Y,Akkaya M,Al Souz J,Holstein AH,Kamenyeva O,Kabat J,Matsumura R,Dorward DW,Glass DD,Shevach EM

12-0541-81 was used in Flow Cytometry to study whether pathways similar to the multiple suppressive mechansims triggered in vitro by T cell antigen receptor activated regulatory T cells exist in vivo.

Fri Feb 01 00:00:00 UTC 2019

Conserved regulation of RNA processing in somatic cell reprogramming.

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Thu Jan 31 00:00:00 UTC 2019

Bovine κ-Casein Fragment Induces Hypo-Responsive M2-Like Macrophage Phenotype.

Nutrients

Lalor R,O'Neill S

12-0541 was used in Flow cytometry/Cell sorting to investigate the possible therapeutic effects bovine casein derived nutraceuticals exert on macrophage immunological function.

Tue Jul 23 00:00:00 UTC 2019

Persistence of Integrase-Deficient Lentiviral Vectors Correlates with the Induction of STING-Independent CD8⁺ T Cell Responses.

Cell reports

Cousin C,Oberkampf M,Felix T,Rosenbaum P,Weil R,Fabrega S,Morante V,Negri D,Cara A,Dadaglio G,Leclerc C

12-0541 was used in Flow cytometry/Cell sorting to demonstrate that integrase-deficient lentiviral vectors can trigger persistent adaptive response by the mediation of a weak and transient innate response alongside the persistence of the vector in tissues.

Tue Jan 29 00:00:00 UTC 2019

Low-Dose Radiotherapy Has No Harmful Effects on Key Cells of Healthy Non-Inflamed Joints.

International journal of molecular sciences

Deloch L,Rückert M,Fietkau R,Frey B,Gaipl US

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Tue Oct 16 00:00:00 UTC 2018

Low-Dose Radiotherapy Ameliorates Advanced Arthritis in hTNF-α tg Mice by Particularly Positively Impacting on Bone Metabolism.

Frontiers in immunology

Deloch L,Derer A,Hueber AJ,Herrmann M,Schett GA,Wölfelschneider J,Hahn J,Rühle PF,Stillkrieg W,Fuchs J,Fietkau R,Frey B,Gaipl US

12-0541 was used in Flow cytometry/Cell sorting to study the underlying mechanisms by which low-dose radiotherapy mediates anti-inflammatory and pain-reducing effects in non-linear dose response relationships.

Mon Sep 30 00:00:00 UTC 2019

Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming.

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Thu Dec 07 00:00:00 UTC 2017

Galectin-3: A Positive Regulator of Leukocyte Recruitment in the Inflamed Microcirculation.

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Gittens BR,Bodkin JV,Nourshargh S,Perretti M,Cooper D

12-0541 was used in Flow cytometry/Cell sorting to show that mechanisms mediating leukocyte slow rolling and emigration are impaired in Gal-3-/- mice, which could be because of impaired expression of cell adhesion molecules and an altered cell surface glycoproteome.

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Lymphatic Endothelial Cells Control Initiation of Lymph Node Organogenesis.

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Onder L,Mörbe U,Pikor N,Novkovic M,Cheng HW,Hehlgans T,Pfeffer K,Becher B,Waisman A,Rülicke T,Gommerman J,Mueller CG,Sawa S,Scandella E,Ludewig B

12-0541-81 was used in Flow Cytometry to find that initiation of LN development is dependent on LTi-cell-mediated activation of lymphatic endothelial cells (LECs) and that engagement of mesenchymal stromal cells is a succeeding event.

Tue Jul 18 00:00:00 UTC 2017

Neutrophil ageing is regulated by the microbiome.

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Neutrophil ageing is regulated by the microbiome.

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Thu Sep 24 00:00:00 UTC 2015

The olive oil-based lipid clinoleic blocks leukocyte recruitment and improves survival during systemic inflammation: a comparative in vivo study of different parenteral lipid emulsions.

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Mon Jan 11 00:00:00 UTC 2016

Reprogramming Roadblocks Are System Dependent.

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CLEC-2 is required for development and maintenance of lymph nodes.

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12-0541 was used in Flow cytometry/Cell sorting to investigate the role that CLEC-2 plays in the development and maintenance of lymph nodes.

Thu May 15 00:00:00 UTC 2014

Dietary gluten increases natural killer cell cytotoxicity and cytokine secretion.

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Larsen J,Dall M,Antvorskov JC,Weile C,Engkilde K,Josefsen K,Buschard K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 12-0541-81) in Flow Cytometry

Wed Oct 01 00:00:00 UTC 2014

RAGE controls activation and anti-inflammatory signalling of protein C.

PloS one

Braach N,Frommhold D,Buschmann K,Pflaum J,Koch L,Hudalla H,Staudacher K,Wang H,Isermann B,Nawroth P,Poeschl J

12-0541 was used in Flow cytometry/Cell sorting to provide the first evidence of interplay between the receptor for advanced glycation endproducts (RAGE) and the protein C pathway in inflammation.

Mon Dec 22 00:00:00 UTC 2014

High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.

Nature

O'Malley J,Skylaki S,Iwabuchi KA,Chantzoura E,Ruetz T,Johnsson A,Tomlinson SR,Linnarsson S,Kaji K

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Thu Jul 04 00:00:00 UTC 2013

The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.

Immunity

Suzuki K,Maruya M,Kawamoto S,Sitnik K,Kitamura H,Agace WW,Fagarasan S

12-0541 was used in Flow cytometry/Cell sorting to investigate whether follicular dendritic cells within the germinal centers directly contribute to the IgA production in Peyer's patches, showing that sensing of environmental stimuli promotes IgA production.

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Stress-regulated targeting of the NKG2D ligand Mult1 by a membrane-associated RING-CH family E3 ligase.

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Nice TJ,Deng W,Coscoy L,Raulet DH

12-0541 was used in Flow cytometry/Cell sorting to investigate the regulation of cell surface expression of the NKG2D ligand, Mult1.

Mon Nov 01 00:00:00 UTC 2010

Ontogeny of stromal organizer cells during lymph node development.

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Bénézech C,White A,Mader E,Serre K,Parnell S,Pfeffer K,Ware CF,Anderson G,Caamaño JH

12-0541 was used in Immunofluorescence to highlight the importance of the signals and cellular interactions that induce the maturation of stromal cells.

Thu Apr 15 00:00:00 UTC 2010

Ontogeny of stromal organizer cells during lymph node development.

Journal of immunology (Baltimore, Md. : 1950)

Bénézech C,White A,Mader E,Serre K,Parnell S,Pfeffer K,Ware CF,Anderson G,Caamaño JH

12-0541 was used in Immunofluorescence to highlight the importance of the signals and cellular interactions that induce the maturation of stromal cells.

Thu Apr 15 00:00:00 UTC 2010

Absence of IFN-beta impairs antigen presentation capacity of splenic dendritic cells via down-regulation of heat shock protein 70.

Journal of immunology (Baltimore, Md. : 1950)

Zietara N,Łyszkiewicz M,Gekara N,Puchałka J,Dos Santos VA,Hunt CR,Pandita TK,Lienenklaus S,Weiss S

12-0541 was used in Flow cytometry/Cell sorting to investigate the role of low level production of IFN, showing that its absence impairs antigen presentation capacity of splenic dendritic cells.

Wed Jul 15 00:00:00 UTC 2009

A role for cFLIP in B cell proliferation and stress MAPK regulation.

Journal of immunology (Baltimore, Md. : 1950)

Zhang H,Rosenberg S,Coffey FJ,He YW,Manser T,Hardy RR,Zhang J

12-0541 was used in Flow cytometry/Cell sorting to reveal the novel functions of cFLIP in B cells.

Thu Jan 01 00:00:00 UTC 2009

Interleukin-10 overexpression promotes Fas-ligand-dependent chronic macrophage-mediated demyelinating polyneuropathy.

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Dace DS,Khan AA,Stark JL,Kelly J,Cross AH,Apte RS

12-0541 was used in Immunohistochemistry on paraffin embedded tissues to investigate the conditions contributing to the development of CIDP and related conditions.

Tue Sep 22 00:00:00 UTC 2009

Endothelial CD47 interaction with SIRPgamma is required for human T-cell transendothelial migration under shear flow conditions in vitro.

Blood

Stefanidakis M,Newton G,Lee WY,Parkos CA,Luscinskas FW

12-0541 was used in Flow cytometry/Cell sorting to test whether CD47-SIRPgamma interactions affect T-cell transmigration using blocking mAb against CD47 or SIRPgamma.

Fri Aug 15 00:00:00 UTC 2008

Lymphotoxin a-dependent and -independent signals regulate stromal organizer cell homeostasis during lymph node organogenesis.

Blood

White A,Carragher D,Parnell S,Msaki A,Perkins N,Lane P,Jenkinson E,Anderson G,Caamaño JH

12-0541 was used in Flow cytometry/Cell sorting to further investigate the mechanisms that regulate the development of stromal organiser cells.

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The Fas-associated death domain protein is required in apoptosis and TLR-induced proliferative responses in B cells.

Journal of immunology (Baltimore, Md. : 1950)

Imtiyaz HZ,Rosenberg S,Zhang Y,Rahman ZS,Hou YJ,Manser T,Zhang J

12-0541 was used in Flow cytometry/Cell sorting to show that the deletion of the Fas-associated death domain protein at the pro-B cell stage has effects on B cell apoptosis and development.

Thu Jun 01 00:00:00 UTC 2006

T/B lineage choice occurs prior to intrathymic Notch signaling.

Blood

Harman BC,Jenkinson WE,Parnell SM,Rossi SW,Jenkinson EJ,Anderson G

12-0541 was used in Flow cytometry/Cell sorting to analyse the T-cell/B-cell lineage potential of precursors that have been recruited to the foetal mouse thymus, showing that lineage choice occurs prior to intrathymic Notch signaling.

Mon Aug 01 00:00:00 UTC 2005

生物信息学

蛋白别名: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

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基因别名: CD54; Icam-1; Icam1; Ly-47; MALA-2

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UniProt ID: (Mouse) P13597

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Entrez Gene ID: (Mouse) 15894

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功能

integrin binding protein complex binding

参与通路