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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • ICAM-1 (CD54) Antibodies

          Invitrogen

          CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), PE, eBioscience™

          9个已发表图片
          31 篇参考文献
          查看其他113个ICAM-1 (CD54)抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), PE, eBioscience™

          • 抗体检测数据 (1)
          • 已发表图片 (9)
          CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.
          CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)
          Group 53 Created with Sketch.

          图: 1 / 10

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          CD54 (ICAM-1) Antibody (12-0541-81) in Flow

          Staining of C57BL/6 splenocytes with 0.06 µg of Rat IgG2b kappa Isotype Control PE (Product # 12-4031-82) (open histogram) or 0.06 µg of Anti-Mouse CD54 (ICAM-1) PE (filled histogram). Total viable cells were used for analysis. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)
          CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)
          CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)
          CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)
          CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)
          CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)
          CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)
          CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)
          CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)
          CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)

          产品信息

          12-0541-81

          应用
          建议稀释比
          已发表文章

          免疫组化 (IHC)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫组化(石蜡) (IHC (P))

          -
          查看1篇已发表文章 1篇已发表文章

          免疫细胞化学 (ICC/IF)

          -
          查看2篇已发表文章 2篇已发表文章

          流式细胞分析 (Flow)

          0.125 µg/test
          查看27篇已发表文章 27篇已发表文章
          产品规格

          种属反应

          Mouse

          已发表种属

          Mouse

          宿主/亚型

          Rat / IgG2b, kappa

          推荐的同型对照

          Rat IgG2b kappa Isotype Control (eB149/10H5), PE, eBioscience™

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          YN1/1.7.4

          偶联物

          PE PE PE
          • Unconjugated
          • Biotin 
          • FITC
          • Functional Grade
          • NovaFluor Red 685
          • NovaFluor Red 700
          • NovaFluor Red 755
          • NovaFluor Yellow 700
          • Request custom conjugation

          激发/发射光谱

          565/576 nm 查看光谱 spectra

          形式

          Liquid

          浓度

          0.2 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2

          内含物

          0.09% sodium azide

          保存条件

          4°C, store in dark, DO NOT FREEZE!

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_465706

          产品详细信息

          Description: The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody.

          Applications Reported: The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis.

          Applications Tested: The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

          Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

          Filtration: 0.2 µm post-manufacturing filtered.

          靶标信息

          ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          How to use the Panel Builder

          Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

          生物信息学

          蛋白别名: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

          查看更多 收起

          基因别名: CD54; Icam-1; Icam1; Ly-47; MALA-2

          查看更多 收起

          UniProt ID: (Mouse) P13597

          查看更多 收起

          Entrez Gene ID: (Mouse) 15894

          查看更多 收起

          功能
          integrin binding protein complex binding
          参与通路
          T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell acute inflammatory response to antigenic stimulus T cell antigen processing and presentation positive regulation of cellular extravasation cell adhesion leukocyte cell-cell adhesion sensory perception of sound regulation of cell shape single organismal cell-cell adhesion regulation of cell adhesion positive regulation of actin filament polymerization cell adhesion mediated by integrin positive regulation of GTPase activity adhesion of symbiont to host positive regulation of nitric oxide biosynthetic process positive regulation of vasoconstriction receptor-mediated virion attachment to host cell positive regulation of peptidyl-tyrosine phosphorylation positive regulation of NF-kappaB transcription factor activity negative regulation of calcium ion transport establishment of endothelial barrier positive regulation of ERK1 and ERK2 cascade cellular response to glucose stimulus establishment of Sertoli cell barrier regulation of ruffle assembly negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of endothelial cell apoptotic process
          It has to be done as per old AB suggested Products section.
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          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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