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CD56 Monoclonal Antibody (123C3.D5)
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产品信息
Z2038MP
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
123C3.D5
抗原
Membrane preparation of a small cell lung carcinoma
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
纯化类型
Protein A
保存液
tris with NP-40, BSA
内含物
<0.1% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
This product is diluted and in a ready-to-use formulation.
A recommended positive control tissue for this product is ZMTB-5, however positive controls are not limited to this tissue type.
The primary antibody is intended for laboratory professional use in the detection of the corresponding protein in formalin-fixed, paraffin-embedded tissue stained in manual qualitative immunohistochemistry (IHC) testing. This antibody is intended to be used after the primary diagnosis of tumor has been made by conventional histopathology using non-immunological histochemical stains.
Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135 kDa isoform is the basic molecule which is glycosylated or sialylated to produce the mature species. NCAM (CD56) is reported to express on most neuroectodermal derived cell lines, tissues, and neoplasms such as retinoblastoma, medulloblastoma, astrocytoma, and neuroblastoma. It is also expressed on some mesodermally derived tumors such as rhabdomyosarcoma and also on natural killer cells.
Antibody is used with formalin-fixed and paraffin-embedded sections. Pretreatment of deparaffinized tissue with heat-induced epitope retrieval or enzymatic retrieval is recommended. In general, immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody (link antibody), an enzyme complex and a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. Results are interpreted using a light microscope and aid in the differential diagnosis of pathophysiological processes, which may or may not be associated with a particular antigen.
A positive tissue control must be run with every staining procedure performed. This tissue may contain both positive and negative staining cells or tissue components and serve as both the positive and negative control tissue. External Positive control materials should be fresh autopsy/biopsy/surgical specimens fixed, processed and embedded as soon as possible in the same manner as the patient sample (s). Positive tissue controls are indicative of correctly prepared tissues and proper staining methods. The tissues used for the external positive control materials should be selected from the patient specimens with well-characterized low levels of the positive target activity that gives weak positive staining. The low level of positivity for external positive controls is designed to ensure detection of subtle changes in the primary antibody sensitivity from instability or problems with the staining methodology. A tissue with weak positive staining is more suitable for optimal quality control and for detecting minor levels of reagent degradation.
Internal or external negative control tissue may be used depending on the guidelines and policies that govern the organization to which the end user belongs to. The variety of cell types present in many tissue sections offers internal negative control sites, but this should be verified by the user. The components that do not stain should demonstrate the absence of specific staining, and provide an indication of non-specific background staining. If specific staining occurs in the negative tissue control sites, results with the patient specimens must be considered invalid.
靶标信息
CD56, also known as neural cell adhesion molecule (NCAM), is a highly glycosylated transmembrane glycoprotein of the immunoglobulin family. It plays a crucial role in cell adhesion, migration, axonal growth, pathfinding, and synaptic plasticity. CD56 is ubiquitously expressed in the nervous system in isoforms ranging from 120-180 kDa and is involved in homotypic adhesion of neural cells. It mediates interactions by binding extracellular matrix components such as laminin and integrins, with polysialic modification reducing CD56-mediated adhesion. In the hematopoietic system, CD56 is expressed on natural killer (NK) cells and a subset of T cells known as NKT cells. It is also found on most neuroectodermal-derived cell lines, tissues, and neoplasms, including retinoblastoma, medulloblastoma, astrocytomas, and neuroblastoma. CD56 serves as a widely used neuroendocrine marker with high sensitivity for neuroendocrine tumors and ovarian granulosa cell tumors. Diseases associated with CD56 dysfunction include rabies and blastic plasmacytoid dendritic cell neoplasms, highlighting its importance in both neural and immune system functions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 145 kda neural cell adhesion molecule; antigen recognized by monoclonal antibody 5.1H11; CD-56; CD56; E NCAM; membrane glycoprotein; N CAM1; N-CAM; N-CAM-1; neural cell adhesion molecule; Neural cell adhesion molecule 1; neural cell adhesion molecule secreted isoform; neural cell adhesion molecule, NCAM; sCD56; sNCAM; soluble CD56; soluble NCAM; unnamed protein product
基因别名: CD56; MSK39; NCAM; NCAM1
UniProt ID: (Human) P13591
Entrez Gene ID: (Human) 4684
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