Invitrogen

CD59 Monoclonal Antibody (MEM-43)

产品信息

MA1-82206

应用	建议稀释比	已发表文章
免疫印迹 (WB)	Assay-dependent	查看1篇已发表文章 1篇已发表文章
免疫组化（石蜡） (IHC (P))	Assay-dependent	-
免疫组化（冰冻） (IHC (F))	Assay-dependent	-
免疫细胞化学 (ICC/IF)	-	查看1篇已发表文章 1篇已发表文章
流式细胞分析 (Flow)	1/5-1/10	查看5篇已发表文章 5篇已发表文章
酶联免疫吸附实验 (ELISA)	Assay-dependent	-
免疫沉淀 (IP)	Assay-dependent	查看1篇已发表文章 1篇已发表文章
其他PubMed (Misc)	-	查看8篇已发表文章 8篇已发表文章

产品规格
种属反应	Human
已发表种属	Human, Rhesus monkey
宿主/亚型	Mouse / IgG2a
分类	Monoclonal
类型	Antibody
克隆号	MEM-43
抗原	Thymocytes and T lymphocytes.
偶联物	Unconjugated PE
形式	Liquid
浓度	1 mg/mL
纯化类型	Protein A
保存液	PBS
内含物	no preservative
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_929142

产品详细信息

In Western blot, this product recognizes CD59 under non-reducing conditions. A suggested positive control for immunohistochemical applications is tonsil. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

靶标信息

CD59 (Protectin) is a small (18-20 kDa) GPI-anchored ubiquitously expressed inhibitor of the membrane attack complex (MAC). CD59 is the key regulator that preserves the autologous cells from terminal effector mechanism of the complement cascade. CD59 also associates with C5b-8 complex and counteracts appropriate formation of cytolytic pore within the plasma membrane. Further, CD59 is a low-affinity ligand of human CD2, causes T cell costimulation, and is involved in lymphocyte signal transduction. CD59 is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. Mutations in this gene cause CD59 deficiency, a disease involving hemolytic anemia, thrombosis, and cerebral infarction. Multiple alternatively spliced transcript variants of CD59 have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

KeepEX, a simple dilution protocol for improving extracellular vesicle yields from urine.

European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences

Puhka M,Nordberg ME,Valkonen S,Rannikko A,Kallioniemi O,Siljander P,Af Hällström TM

MA182206 was used in western blot to discuss methods to increase the urinary extracellular vesicle yield

Wed Feb 15 00:00:00 EST 2017

TNF Regulates Essential Alternative Complement Pathway Components and Impairs Activation of Protein C in Human Glomerular Endothelial Cells.

Journal of immunology (Baltimore, Md. : 1950)

Sartain SE,Turner NA,Moake JL

MA1-82206 was used in immunocytochemistry to study the essential alternative complement pathway components regulated by TNF and how they impair activation of protein c in human glomerular endothelial cells

Fri Jan 15 00:00:00 EST 2016

CD59 signaling and membrane pores drive Syk-dependent erythrocyte necroptosis.

Cell death & disease

LaRocca TJ,Stivison EA,Mal-Sarkar T,Hooven TA,Hod EA,Spitalnik SL,Ratner AJ

MA182206 was used in immunoprecipitation to study mechanisms of programed cell death of red blood cells

Thu May 28 00:00:00 EDT 2015

Identification of a cross-reacting, monoclonal anti-human CD233 antibody for identification and sorting of rhesus macaque erythrocytes.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Byrnes C,Terry Lee Y,Donahue RE,Miller JL

MA1-82206 was used in flow cytometry to use cross reactive antibodies to identify rhesus macaque erythrocytes.

Wed Feb 01 00:00:00 EST 2012

Human placenta and chorion: potential additional sources of hematopoietic stem cells for transplantation.

Transfusion

Bárcena A,Muench MO,Kapidzic M,Gormley M,Goldfien GA,Fisher SJ

MA1-82206 was used in immunocytochemistry to discuss the use of umbilical cord blood as a source of transplantable hematopoietic stem cells.

Tue Nov 01 00:00:00 EDT 2011

Guidelines for the diagnosis and monitoring of paroxysmal nocturnal hemoglobinuria and related disorders by flow cytometry.

Cytometry. Part B, Clinical cytometry

Borowitz MJ,Craig FE,Digiuseppe JA,Illingworth AJ,Rosse W,Sutherland DR,Wittwer CT,Richards SJ

MA1-82206 was used in flow cytometry to discuss ways to standardize flow cytometry methods to diagnose paroxysmal nocturnal hemoglobinuria

Thu Jul 01 00:00:00 EDT 2010

Copy number of FCGR3B, which is associated with systemic lupus erythematosus, correlates with protein expression and immune complex uptake.

The Journal of experimental medicine

Willcocks LC,Lyons PA,Clatworthy MR,Robinson JI,Yang W,Newland SA,Plagnol V,McGovern NN,Condliffe AM,Chilvers ER,Adu D,Jolly EC,Watts R,Lau YL,Morgan AW,Nash G,Smith KG

MA1-82206 was used in flow cytometry to examine how FCGR3B copy number affects immune responses

Mon Jul 07 00:00:00 EDT 2008

Thrice-weekly low-dose rituximab decreases CD20 loss via shaving and promotes enhanced targeting in chronic lymphocytic leukemia.

Journal of immunology (Baltimore, Md. : 1950)

Williams ME,Densmore JJ,Pawluczkowycz AW,Beum PV,Kennedy AD,Lindorfer MA,Hamil SH,Eggleton JC,Taylor RP

MA1-82206 was used in flow cytometry to examine loss of CD20 in chronic lymphocytic leukemia

Wed Nov 15 00:00:00 EST 2006

Signature of B-CLL with different prognosis by Shrunken centroids of surface antigen expression profiling.

Journal of cellular physiology

Zucchetto A,Sonego P,Degan M,Bomben R,Dal Bo M,Russo S,Attadia V,Rupolo M,Buccisano F,Del Principe MI,Del Poeta G,Pucillo C,Colombatti A,Campanini R,Gattei V

MA1-82206 was used in flow cytometry to use flow cytometry to characterize 123 cases of B-cell chronic lymphocytic leukemia

Fri Jul 01 00:00:00 EDT 2005

Identification of unsuspected PNH-type cells in flow cytometric immunophenotypic analysis of peripheral blood and bone marrow.

American journal of clinical pathology

Thomason RW,Papiez J,Lee RV,Szczarkowski W

MA1-82206 was used in flow cytometry to use flow cytometry to characterize cells from patients with paroxysmal nocturnal hemoglobinuria

Thu Jul 01 00:00:00 EDT 2004

Searching for common stem cells of the hepatic and hematopoietic systems in the human fetal liver: CD34+ cytokeratin 7/8+ cells express markers for stellate cells.

Journal of hepatology

Suskind DL,Muench MO

MA1-82206 was used in flow cytometry to test if human fetal liver cells expressing CD34 and CK7/8 represent a common stem cell for both the hematopoietic and hepatic systems

Sun Feb 01 00:00:00 EST 2004

Systemic therapy with cyclophosphamide and anti-CD20 antibody (rituximab) in relapsed primary cutaneous B-cell lymphoma: a report of 7 cases.

Journal of the American Academy of Dermatology

Fierro MT,Savoia P,Quaglino P,Novelli M,Barberis M,Bernengo MG

MA1-82206 was used in immunohistochemistry to discuss the use of Rituximab.

Fri Aug 01 00:00:00 EDT 2003

The association between glycosylphosphatidylinositol-anchored proteins and heterotrimeric G protein alpha subunits in lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America

Solomon KR,Rudd CE,Finberg RW

MA1-82206 was used in immunoprecipitation to report that GPI-anchored proteins associate with G proteins.

Tue Jun 11 00:00:00 EDT 1996

Complement regulatory molecules on human myelin and glial cells: differential expression affects the deposition of activated complement proteins.

Journal of neurochemistry

Koski CL,Estep AE,Sawant-Mane S,Shin ML,Highbarger L,Hansch GM

MA1-82206 was used in western blot to study CD55 and CD59 in Schwann cells.

Mon Jan 01 00:00:00 EST 1996

Phenotypic and functional analysis of lymphocytes in paroxysmal nocturnal hemoglobinuria.

American journal of hematology

Tseng JE,Hall SE,Howard TA,Ware RE

MA1-82206 was used in immunoprecipitation to determine the lymphoid lineage involvement in paroxysmal nocturnal hemoglobinuria.

Fri Dec 01 00:00:00 EST 1995

The CD59 antigen is a structural homologue of murine Ly-6 antigens but lacks interferon inducibility.

European journal of immunology

Philbrick WM,Palfree RG,Maher SE,Bridgett MM,Sirlin S,Bothwell AL

MA1-82206 was used in flow cytometry to study expression of CD59.

Mon Jan 01 00:00:00 EST 1990

生物信息学

蛋白别名: 1F5 antigen; 20 kDa homologous restriction factor; CD59; CD59 antigen p18-20 (antigen identified by monoclonal antibodies 16.3A5, EJ16, EJ30, EL32 and G344); CD59 glycoprotein; CD59 molecule, complement regulatory protein; HRF-20; human leukocyte antigen MIC11; Ly-6-like protein; lymphocytic antigen CD59/MEM43; MAC-inhibitory protein; MAC-IP; MACIF; MEM43 antigen; membrane attack complex (MAC) inhibition factor; Membrane attack complex inhibition factor; Membrane inhibitor of reactive lysis; MIRL; Protectin; surface anitgen recognized by monoclonal antibody 16.3A5; T cell-activating protein

基因别名: 16.3A5; 1F5; CD59; EJ16; EJ30; EL32; G344; HRF-20; HRF20; MAC-IP; MACIF; MEM43; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21; p18-20

UniProt ID: (Human) P13987

Entrez Gene ID: (Human) 966

功能

cell activation negative regulation of activation of membrane attack complex ER to Golgi vesicle-mediated transport cell surface receptor signaling pathway blood coagulation regulation of complement activation positive regulation of T cell proliferation negative regulation of apoptotic process COPII vesicle coating

参与通路

complement binding protein binding

CD59 Monoclonal Antibody (MEM-43)

产品信息

MA1-82206

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫组化（冰冻） (IHC (F))

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

酶联免疫吸附实验 (ELISA)

免疫沉淀 (IP)

其他PubMed (Misc)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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