Invitrogen

CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), NovaFluor™ Blue 660-120S, eBioscience™

Name: CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), NovaFluor\u2122 Blue 660-120S, eBioscience\u2122
Brand: Invitrogen
SKU: M053T03B08-A

查看其他79个CD61 (Integrin beta 3)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)

CD61 (Integrin beta 3) Antibody in Flow Cytometry (Flow)

图: 1 / 2

CD61 (Integrin beta 3) Antibody (M053T03B08-A) in Flow

C57BL/6 mouse bone marrow were either left unstained (blue histogram) or stained with 0.2 µg of CD61 (Integrin beta 3) Monoclonal Antibody, NovaFluor Blue 660-120S (purple histogram). Total viable cells in the lymphoid (left) or myeloid (right) gates were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extra... View More

Product Image Gallery

产品信息

M053T03B08-A

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.2 µg/test

产品规格

种属反应

Mouse, Rat

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

2C9.G3

偶联物

NovaFluor™ Blue 660-120S NovaFluor™ Blue 660-120S NovaFluor™ Blue 660-120S

激发/发射光谱

492/665 nm 查看光谱

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

AB_3099248

产品详细信息

Description
The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3.

This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

Applications Tested
The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.2 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Master mixes
• Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time.
• We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer.

Whole Blood compatibility
• When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates.
• See the Bulk Lysis of Human Whole Blood protocol here.
• Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-than-expected background staining.

Viability dye compatibility
• NovaFluor dyes are not compatible with DNA intercalating viability dyes.
• Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.

CellBlox Plus Blocking Buffer
• This NovaFluor conjugate comes with CellBlox Plus Blocking Buffer (for 25 µg or 25 test products, Cat. No. C001T02F01; for 100 µg or 100 test, Cat. No. C001T03F01), essential for optimal staining.
• Use CellBlox Plus Blocking Buffer in all experiments with NovaFluor conjugates.
• Add 5 µL per sample to antibody cocktails/master mixes (regardless of how many Novafluor-conjugated antibodies are present) before combining with cells.
• CellBlox Plus Blocking Buffer is compatible with either Super Bright Complete Blocking Buffer or Brilliant Stain Buffer and can be used in antibody cocktails/master mixes with those reagents.
• For single-color controls, use 5 µL of CellBlox Plus Blocking Buffer per 100 µL of cell sample (10^3 to 10^8 cells).

NovaFluor conjugates are based on Phiton technology utilizing novel fluorophore-containing nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more

Excitation: 508 nm; Emission: 664 nm; Laser: 488 nm (Blue) Laser

靶标信息

CD61, also known as GPIIIa or ITGB3, is a 105 kDa glycoprotein expressed on activated T cells, granulocytes, megakaryocytes, platelets, and their precursors. It plays a crucial role in platelet aggregation and functions as a receptor for fibrinogen, fibronectin, von Willebrand factor, vitronectin, and thrombospondin. CD61 forms heterodimeric complexes by associating non-covalently with integrin alpha subunits: alphaV (CD51) to create the Vitronectin Receptor and alphaIIb (CD41) to form gpIIb/IIIa. These complexes are responsible for adhesion to extracellular matrix components, facilitating cell adhesion and cell-surface mediated signaling. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, and osteoclasts in association with CD51. The protein product of CD61 is composed of an alpha chain and a beta chain, which can combine with multiple partners to form different integrins. Its involvement in cell adhesion and signaling underscores its importance in normal physiological processes. Dysfunction of CD61 is associated with diseases such as Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder, highlighting its critical role in hemostasis and platelet function.

仅用于科研。不用于诊断过程。未经明确授权不得转售。