Invitrogen

CD62E (E-selectin) Monoclonal Antibody (P2H3), eBioscience™

CD62E (E-selectin) Antibody in Western Blot (WB)

图: 1 / 5

CD62E (E-selectin) Antibody (14-0627-82) in WB

Western blot was performed using Anti-CD62E (E-selectin) Monoclonal Antibody (Product # 14-0627-82) and 67, 115 kDa bands corresponding to CD62E were observed across the tissues tested. Tissue extracts (30 µg lysate) of Mouse Liver (Lane 1), Mouse Spleen (Lane 2) and Mouse White Adipose (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed... View More

CD62E (E-selectin) Antibody in Flow Cytometry (Flow)

Product Image Gallery

产品信息

14-0627-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

免疫组化 (IHC)

Assay-Dependent

流式细胞分析 (Flow)

1 µg/test

查看3篇已发表文章 3篇已发表文章

酶联免疫吸附实验 (ELISA)

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

Assay-Dependent

中和实验 (Neu)

查看1篇已发表文章 1篇已发表文章

功能检测 (Functional)

Assay-Dependent

查看1篇已发表文章 1篇已发表文章

抑制试剂 (IA)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human, Mouse

已发表种属

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

P2H3

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_1210768

产品详细信息

Description: The P2H3 monoclonal antibody reacts with human CD62E, a 97-115 kDa member of the selectin family. CD62E, also known as E-selectin or endothelial-leukocyte adhesion molecule-1 (ELAM-1) is an adhesion molecule expressed by endothelial cells upon stimulation with cytokines including TNFalpha and IL-1beta. Induced expression of CD62E during inflammatory conditions is thought to mediate leukocyte rolling including the initial interaction of neutrophils with endothelium.

The P2H3 monoclonal antibody also inhibits cellular adhesion to cytokine-activated endothelial cells.

Applications Reported: This P2H3 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining.

Applications Tested: This P2H3 antibody has been tested by flow cytometric analysis of TNF alpha-actiavted Human Umbilical Vein Endothelial Cells (HUVEC). This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Development of an In Vitro Blood Vessel Model Using Autologous Endothelial Cells Generated from Footprint-Free hiPSCs to Analyze Interactions of the Endothelium with Blood Cell Components and Vascular Implants.

Cells

Weber J,Weber M,Feile A,Schlensak C,Avci-Adali M

Published figure using CD62E (E-selectin) monoclonal antibody (Product # 14-0627-82) in Flow Cytometry

Sat Apr 22 00:00:00 UTC 2023

Surgical and intravital microscopy protocol to image Trypanosoma brucei-host interactions in live rodent models.

STAR protocols

De Niz M,Figueiredo LM

14-0627-82 was used in inhibition assays to provide an IVM protocol for time-lapse imaging of dynamic Trypanosoma brucei-host interactions in ten mammalian organs and in systemic circulation.

Fri Jun 17 00:00:00 UTC 2022

A Norrin/Wnt surrogate antibody stimulates endothelial cell barrier function and rescues retinopathy.

EMBO molecular medicine

Chidiac R,Abedin M,Macleod G,Yang A,Thibeault PE,Blazer LL,Adams JJ,Zhang L,Roehrich H,Jo HN,Seshagiri S,Sidhu SS,Junge HJ,Angers S

14-0627-82 was used in Functional assay to develop F4L5.13, a tetravalent antibody designed to induce FZD4 and LRP5 proximity in such a way as to trigger βcatenin signaling.

Wed Jul 07 00:00:00 UTC 2021

Organotypic endothelial adhesion molecules are key for Trypanosoma brucei tropism and virulence.

Cell reports

De Niz M,Brás D,Ouarné M,Pedro M,Nascimento AM,Henao Misikova L,Franco CA,Figueiredo LM

14-0627-82 was used in Inhibition Assays to investigate the importance of the vasculature in tissue tropism.

Tue Sep 21 00:00:00 UTC 2021

Colonization of dermal arterioles by Neisseria meningitidis provides a safe haven from neutrophils.

Nature communications

Manriquez V,Nivoit P,Urbina T,Echenique-Rivera H,Melican K,Fernandez-Gerlinger MP,Flamant P,Schmitt T,Bruneval P,Obino D,Duménil G

14-0627-82 was used in Flow Cytometry to demonstrate how neutrophil recruitment contributes to resolution of Neisseria meningitidis in a humanized mouse model.

Tue Jul 27 00:00:00 UTC 2021

Long non‑coding RNA DILC is involved in sepsis by modulating the signaling pathway of the interleukin‑6/signal transducer and activator of transcription 3/Toll‑like receptor 4 axis.

Molecular medicine reports

Huang W,Huang L,Wen M,Fang M,Deng Y,Zeng H

14-0627-82 was used in ELISA to investigate the role of deleted in liver cancer 1 (DILC), interleukin (IL)‚6, signal transducer and activator of transcription 3 (STAT3), and Toll‚like receptor 4 (TLR4) in the pathogenesis of sepsis.

Sat Dec 01 00:00:00 UTC 2018

Using CRISPR-Cas9 to quantify the contributions of O-glycans, N-glycans and Glycosphingolipids to human leukocyte-endothelium adhesion.

Scientific reports

Stolfa G,Mondal N,Zhu Y,Yu X,Buffone A,Neelamegham S

14-0627 was used in Blocking experiments to develop a CRISPR-Cas9 toolkit to selectively truncate N-glycans, O-glycans and glycosphingolipids.

Tue Jul 26 00:00:00 UTC 2016

Effect of nicotine and porphyromonas gingivalis lipopolysaccharide on endothelial cells in vitro.

PloS one

An N,Andrukhov O,Tang Y,Falkensammer F,Bantleon HP,Ouyang X,Rausch-Fan X

Published figure using CD62E (E-selectin) monoclonal antibody (Product # 14-0627-82) in Flow Cytometry

Mon Jun 29 00:00:00 UTC 2015

生物信息学

蛋白别名: CD62 antigen-like family member E; CD62E; e selectin; E-selectin; ELAM-1; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; RP1-117P20.2

查看更多收起

基因别名: CD62E; E-selectin; ELAM; Elam-1; ELAM1; ESEL; LECAM2; SELE

查看更多收起

UniProt ID: (Human) P16581, (Mouse) Q00690

查看更多收起

Entrez Gene ID: (Human) 6401, (Mouse) 20339

查看更多收起

功能

transmembrane signaling receptor activity protein binding sialic acid binding phospholipase binding oligosaccharide binding carbohydrate binding

参与通路