Invitrogen

CD62P (P-Selectin) Monoclonal Antibody (Psel.KO2.3), Super Bright™ 600, eBioscience™

CD62P (P-Selectin) Antibody in Flow Cytometry (Flow)

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CD62P (P-Selectin) Antibody (63-0626-82) in Flow

Human platelets were unstimulated (left) or activated by thrombin (right) and stained with CD42b Monoclonal Antibody, PE (Product # 12-0429-42) and Mouse IgG1 kappa Isotype Control, Super Bright 600 (Product # 63-4714-82) (top) or CD62P Monoclonal Antibody, Super Bright 600 (bottom). Total cells were used for analysis.

CD62P (P-Selectin) Antibody in Immunocytochemistry (ICC/IF)

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产品信息

63-0626-82

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

0.5 µg/test

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产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

Psel.KO2.3

偶联物

Super Bright™ 600 Super Bright™ 600 Super Bright™ 600

激发/发射光谱

414/601 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with BSA

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2802431

产品详细信息

Description: The Psel.KO2.3 monoclonal antibody reacts with mouse CD62P (also known as P-selectin and GMP-140), a 140-kDa cell adhesion molecule that can be induced on endothelial cells and platelets. During inflammation, this selectin mediates adhesion of monocytes and neutrophils to activated platelets, as well as leukocyte rolling on endothelial cells. The major ligand for CD62P on neutrophils is P-selectin glycoprotein ligand-1 (PSGL-1). This monoclonal antibody recognizes an epitope that is conserved across several species (e.g. mouse, human, and rat) and has not been reported to block cell adhesion.

Applications Reported: This Psel.KO2.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This Psel.KO2.3 antibody has been tested by flow cytometric analysis of thrombin-activated platelets. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

靶标信息

P-selectin (Selectin P, GMP-140, LECAM3, CD62 antigen-like family member) is a 140 kDa type 1 transmembrane glycoprotein, and is one of the most commonly studied proteins that regulate cell rolling. P-selectin is stored in the Weibel-Palade bodies of endothelial cells, as well as in a-granules of platelets. From there, it can be rapidly brought to the cell surface after exposure to thrombin, histamine, complement 5a, Ca21 ionophores, or adenosine diphosphate. P-selectin protein redistributes to the plasma membrane during platelet activation and degranulation and mediates the interaction of activated endothelial cells or platelets with leukocytes. P-selectin plays an important role in adhesive processes between leucocytes and endothelial cells, and is a calcium dependent receptor that binds to sialylated forms of Lewis blood group carbohydrate antigens found on neutrophils and monocytes. P-selectin is constitutively expressed in inflammation and contributes to atherogenesis, thrombosis and tissue destruction. Clinically, P-selectin is used to distinguish heparin induced thrombocytopenia with and without thrombosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

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Product References

Myristica fragrans Extract Inhibits Platelet Desialylation and Activation to Ameliorate Sepsis-Associated Thrombocytopenia in a Murine CLP-Induced Sepsis Model.

International journal of molecular sciences

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Small-Molecule Cyclophilin Inhibitors Potently Reduce Platelet Procoagulant Activity.

International journal of molecular sciences

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Longitudinal RNA-Seq Analysis of the Repeatability of Gene Expression and Splicing in Human Platelets Identifies a Platelet SELP Splice QTL.

Circulation research

Rondina MT,Voora D,Simon LM,Schwertz H,Harper JF,Lee O,Bhatlekar SC,Li Q,Eustes AS,Montenont E,Campbell RA,Tolley ND,Kosaka Y,Weyrich AS,Bray PF,Rowley JW

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Loss of Tet2 affects platelet function but not coagulation in mice.

Blood science (Baltimore, Md.)

Wang B,Xia M,Chen T,Li M,Shi D,Wang X,Pang A,Zhou J,Yuan W,Chu Y

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Expression and Clinical Prognostic Value of Platelet NLRP3 in Acute Coronary Syndrome.

International journal of general medicine

Peng H,Wu H,Zhang G,Zhang W,Guo Y,Chang L,Chen S,Xue R,Zhang S

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Sun Apr 17 00:00:00 UTC 2022

Neutrophil-derived S100 calcium-binding proteins A8/A9 promote reticulated thrombocytosis and atherogenesis in diabetes.

The Journal of clinical investigation

Kraakman MJ,Lee MK,Al-Sharea A,Dragoljevic D,Barrett TJ,Montenont E,Basu D,Heywood S,Kammoun HL,Flynn M,Whillas A,Hanssen NM,Febbraio MA,Westein E,Fisher EA,Chin-Dusting J,Cooper ME,Berger JS,Goldberg IJ,Nagareddy PR,Murphy AJ

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The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis.

Nature communications

Qiu C,Wang Y,Zhao H,Qin L,Shi Y,Zhu X,Song L,Zhou X,Chen J,Zhou H,Zhang H,Tellides G,Min W,Yu L

Published figure using CD62P (P-Selectin) monoclonal antibody (Product # 63-0626-82) in Flow Cytometry

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生物信息学

蛋白别名: CD62 antigen-like family member P; CD62P; FLJ45155; GMP-140; Granule membrane protein 140; granule membrane protein 140kDa; granulocyte membrane protein; LECAM3; Leukocyte-endothelial cell adhesion molecule 3; P-selectin; PADGEM; Platelet activation dependent granule-external membrane protein; platelet alpha-granule membrane protein; RP1-86F14.2; selectin P (granule membrane protein 140kDa, antigen CD62)

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基因别名: CD62; CD62P; GMP-140; GMP140; GMRP; GRMP; LECAM3; PADGEM; PSEL; SELP

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UniProt ID: (Human) P16109, (Mouse) Q01102

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Entrez Gene ID: (Human) 6403, (Mouse) 20344

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