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产品信息
11-0791-80
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
流式细胞分析 (Flow) |
1 µg/test | - |
| 产品规格 | |
|---|---|
种属反应 |
Mouse |
宿主/亚型 |
Mouse / IgG1, kappa |
推荐的同型对照 |
Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
24C2.5 |
偶联物 |
FITC
|
激发/发射光谱 |
498/517 nm
查看光谱
|
形式 |
Liquid |
浓度 |
0.5 mg/mL |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.2 |
内含物 |
0.09% sodium azide |
保存条件 |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_1582245 |
产品详细信息
Description: This 24C2.5 monoclonal antibody reacts with the intracellular tail of mouse CD79a (also known as Ig alpha or mb-1), a signaling component of the B cell receptor (BCR). CD79a heterodimerizes with CD79b (Ig beta, B29), and together with surface Ig, make up the BCR. Both CD79 subunits consist of a single extracellular Ig domain, a transmembrane domain, and an intracellular signaling domain. CD79a is expressed almost exclusively on B cells, as well as in most B lineage acute lymphoblastic leukemias. The cytoplasmic domain of CD79a contains immunoreceptor tyrosine-based activation motifs (ITAMs), which constitute the signal transducing portions of the BCR. Tyrosine phosphorylation of these ITAMs by Syk and Lyn initiates numerous signaling cascades, resulting in B cell activation, proliferation, and differentiation.
Applications Reported: This 24C2.5 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This 24C2.5 antibody has been tested by flow cytometric analysis on mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
CD79a is a disulphide-linked heterodimer that includes B29 (CD79b) polypeptide. CD79a is a B lymphocyte antigen receptor with an antigen-specific surface component Ig (immunoglobulin) that associates with Ig-alpha and Ig-beta, necessary elements for the expression and function of the B-cell antigen receptor. CD79a first appears at pre-B cell stage and persists until the plasma cell stage where it is found as an intracellular component. CD79a is found in the majority of acute leukemias of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines. Diseases associated with CD79a include Agammaglobulinemia 3 and Agammaglobulinemia, Non-Bruton type.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
Flow Cytometry
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生物信息学
蛋白别名: B-cell antigen receptor complex-associated protein alpha chain; CD79; CD79a; Ig alpha; Ig-alpha; immunoglobulin-associated alpha; MB-1 membrane glycoprotein; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein
基因别名: Cd79a; Ig-alpha; Iga; Igalpha; Ly-54; Ly54; Mb-1
UniProt ID: (Mouse) P11911
Entrez Gene ID: (Mouse) 12518
分子生物学功能:
immunoglobulin receptor superfamily
