Invitrogen

CD8 Monoclonal Antibody (3B5), APC

Name: CD8 Monoclonal Antibody (3B5), APC
Brand: Invitrogen
SKU: MHCD0805

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19 篇参考文献

查看其他465个CD8抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
已发表图片 (2)

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CD8 Antibody (MHCD0805) in Flow

Human peripheral blood lymphocytes were stained using APC of anti-human CD8 monoclonal antibody (clone 3B5). The negative control profiles represent unstained cells.

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产品信息

MHCD0805

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

Assay-Dependent

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功能检测 (Functional)

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其他PubMed (Misc)

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产品规格

种属反应

Human

已发表种属

C. elegans, Human, Non-human primate

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

3B5

抗原

Human CD8

偶联物

APC APC APC

激发/发射光谱

651/660 nm 查看光谱

形式

Liquid

纯化类型

purified

保存液

PBS with BSA, sucrose

内含物

0.1% sodium azide

保存条件

4°C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_10392701

产品详细信息

Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients.

This antibody recognizes the alpha chain alone as well as the alpha/beta heterodimer.

靶标信息

CD8, also known as cluster of differentiation 8, is a type I transmembrane glycoprotein of the immunoglobulin family that plays a crucial role in T cell differentiation, activation, and signal transduction. It is expressed as either a heterodimer (CD8 alpha beta) or a homodimer (CD8 alpha alpha). The CD8 alpha beta form is predominantly found on the majority of thymocytes and a subpopulation of mature alpha beta TCR T cells, while the CD8 alpha alpha form is expressed on gamma delta TCR T cells, a subset of intestinal intraepithelial lymphocytes (IELs), and dendritic cells. CD8 functions as a co-receptor for major histocompatibility complex class I (MHC-I) molecules, working alongside the T cell receptor (TCR). The CD8 alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes, and dendritic cells as disulfide-linked homodimers of CD8 alpha. Upon ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), CD8 recruits lymphocyte-specific protein tyrosine kinase (Lck), leading to lymphokine production, increased motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for clearing pathogens and tumor cells. The differentiation of naive CD8+ T cells into CTLs is strongly enhanced by cytokines such as IL-2, IL-12, and TGF-beta1. Through its interactions with MHC-I and association with protein tyrosine kinase p56lck, CD8 plays a significant role in T cell development and the activation of mature T cells.

Analyte Specific Reagent

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生物信息学

蛋白别名: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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基因别名: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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功能

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

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