Invitrogen

CD8 Monoclonal Antibody (3B5), Qdot™ 800

FIGURE: 1 / 1

CD8 Antibody (Q22157) in Flow

Histogram of gated human lymphocytes labeled with CD8 Mouse Anti-Human mAb (clone 3B5), Qdot® 800 Conjugate Conjugate. Sample was acquired and analyzed using 405 nm excitation and a 780/60 band pass emission filter using a BD LSR II flow cytometer (BD Biosciences, San Jose, CA).

Product Image Gallery

Product Details

Q22157

Applications

Tested Dilution

Publications

流式细胞分析 (Flow)

Assay-dependent

查看3篇已发表文章 3篇已发表文章

产品规格

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

3B5

Immunogen

Recognizes the CD8 antigen

Conjugate

Qdot™ 800

Excitation/Emission Max

300/792 nm View spectra

形式

Liquid

纯化类型

purified

保存液

0.05M borate, pH 8.3, with 1M betaine

内含物

0.05% sodium azide

保存条件

4° C

RRID

AB_2556513

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Flow Cytometry

Panel Builder

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Clonally expanded CD8 T cells characterize amyotrophic lateral sclerosis-4.

Nature

Campisi L,Chizari S,Ho JSY,Gromova A,Arnold FJ,Mosca L,Mei X,Fstkchyan Y,Torre D,Beharry C,Garcia-Forn M,Jiménez-Alcázar M,Korobeynikov VA,Prazich J,Fayad ZA,Seldin MM,De Rubeis S,Bennett CL,Ostrow LW,Lunetta C,Squatrito M,Byun M,Shneider NA,Jiang N,La Spada AR,Marazzi I

Q22157 was used in Flow cytometry/Cell sorting to provide evidence of an antigen-specific CD8 T cell response in ALS4, which could be used to unravel disease mechanisms and as a potential biomarker of disease state.

Wed Jun 01 00:00:00 EDT 2022

Selective Loss of Early Differentiated, Highly Functional PD1high CD4 T Cells with HIV Progression.

PloS one

Paris RM,Petrovas C,Ferrando-Martinez S,Moysi E,Boswell KL,Archer E,Yamamoto T,Ambrozak D,Casazza JP,Haubrich R,Connors M,Ake J,Kim JH,Koup RA

Q22157 was used in flow cytometry to analyze HIV progression traits of a selective loss of early differentiated and highly functional PD1high CD4 T cells

Tue Jun 28 00:00:00 EDT 2016

Techniques to improve the direct ex vivo detection of low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class I tetramers.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Chattopadhyay PK,Melenhorst JJ,Ladell K,Gostick E,Scheinberg P,Barrett AJ,Wooldridge L,Roederer M,Sewell AK,Price DA

Q22157 was used in flow cytometry to study low frequency antigen-specific CD8+ T cells with peptide-major histocompatibility complex class 1 tetramers and techniques to improve the direct ex vivo detection

Sat Nov 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

View more View less

Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

View more View less

UniProt ID: (Human) P01732, (Human) P10966

View more View less

Entrez Gene ID: (Human) 925, (Human) 926

View more View less

Molecular Function: defense/immunity protein immunoglobulin receptor superfamily

Function(s)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

Process(es)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD8 Monoclonal Antibody (3B5), Qdot™ 800

CD8 Antibody (Q22157) in Flow

Product Details

Q22157

流式细胞分析 (Flow)

Species Reactivity

Published species

Host/Isotype

分类

类型

克隆号

Immunogen

Conjugate

Excitation/Emission Max

形式

纯化类型

保存液

内含物

保存条件

RRID

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!