Invitrogen

CD8 alpha Polyclonal Antibody

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CD8 alpha Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of CD8 alpha on paraffin-embedded human tonsil tissue. Sample was incubated with CD8 alpha polyclonal antibody (Product# PA5-79010).

CD8 alpha Antibody in Western Blot (WB) — Western blot analysis of CD8 alpha in Lane 1: JURKAT whole cell lysate, Lane 2: RAJI whole cell lysate, Lane 3: HL-60 whole cell lysate, Lane 4: A549 whole cell lysate, Lane 5: COLO320 whole cell lysate, Lane 6: HeLa whole cell lysate using 40-50 µg per well. Sample was incubated with CD8 alpha (Product # PA5-79010) at a dilution of 0.5 µg/mL.

产品信息

PA5-79010

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human CD8 alpha recombinant protein (Position: S22-A187).

查看免疫原

偶联物

Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

RRID

AB_2746126

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

靶标信息

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

基因别名: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

UniProt ID: (Human) P01732, (Human) P10966

Entrez Gene ID: (Human) 925, (Human) 926

分子生物学功能: defense/immunity protein immunoglobulin receptor superfamily