Invitrogen

CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), eFluor™ 506, eBioscience™

Product Image Gallery

CD90.2 (Thy-1.2) Antibody in Flow Cytometry (Flow) — BALB/c mouse splenocytes were stained with CD45R (B220) Monoclonal Antibody, APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, eFluor 506 (Product # 69-4321-82) (left) or 0.25 µg of CD90.2 Monoclonal Antibody, eFluor 506 (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50).

CD90.2 (Thy-1.2) Antibody in Immunohistochemistry (IHC) — Fig. 7 Lasp1 affects tissue architecture in the synovium of hTNFtg mice. a H&E stainings (scale bar: 50 um) of paraffin sections from hind paws from 12 weeks old wt, Lasp1 -/- , hTNFtg and hTNFtg /Lasp1 -/- female mice ( n = 3 in each group). Evaluation of synovial cell density was performed by counting cell numbers per high power field (HPF, 10.000 um 2 ) ( n = 10, 3 HPF/animal). Data presented as mean +- SEM, * P < 0.05, ** P < 0.001, *** P < 0.0001 (two-tailed Mann-Whitney U test). b Immunofluorescence staining for PDPN, FAPalpha, THY1 expression and the nuclei with DAPI in white in 12 weeks old joints (representative images of n = 5 per group, scale bar: 10 um). c Immunoblotting and quantification for phospho-specific Src kinase (pSrc 418 ) and total Src kinase expression as well as for phospho-specific AKT kinase (pAKT) and total AKT in FLS from hTNFtg and hTNFtg /Lasp1 -/- mice stimulated with recombinant murine PDGF for the indicated time periods ( n = 3 each group). Source data are provided as a Source Data file.

产品信息

69-0902-82

应用	建议稀释比	已发表文章
免疫组化 (IHC)	-	查看1篇已发表文章 1篇已发表文章
流式细胞分析 (Flow)	0.5 µg/test	查看4篇已发表文章 4篇已发表文章

产品规格
种属反应	Mouse
已发表种属	Not Applicable
宿主/亚型	Rat / IgG2a, kappa
推荐的同型对照	Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 506, eBioscience™
分类	Monoclonal
类型	Antibody
克隆号	53-2.1
偶联物	eFluor™ 506 APC APC-eFluor 780 Biotin Brilliant UV 615 eFluor 450 FITC NovaFluor Blue 510 NovaFluor Blue 555 NovaFluor Blue 610-70S NovaFluor Blue 660-120S NovaFluor Red 710 NovaFluor Yellow 570 NovaFluor Yellow 660 PE PE-Cyanine5 PE-Cyanine7 PE-eFluor 610 Super Bright 436 Super Bright 600 Super Bright 645 Super Bright 702 Unconjugated
激发/发射光谱	419/508 nm 查看光谱
形式	Liquid
浓度	0.2 mg/mL
纯化类型	Affinity chromatography
保存液	PBS, pH 7.2
内含物	0.09% sodium azide
保存条件	4° C, store in dark, DO NOT FREEZE!
RRID	AB_2762804

产品详细信息

Description: The 53-2.1 monoclonal antibody reacts with mouse CD90.2 also known as Thy-1.2, a GPI-linked membrane molecule. CD90.2 is expressed by mouse thymocytes and mature T cells as well as neurons in CD90.2-expressing mouse strains. These strains include BALB/c, CBA, C3H, C57BL/6, C58/, SJL and others. Cells from CD90.1-expressing strains including PL and AKR do not stain with 53-2.1. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: This 53-2.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 53-2.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor™ 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser

靶标信息

CD90.2 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90.2 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. Thy 1 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90.2 is an 18 kDa plasma membrane antigen expressed by hematopoietic cells and neurons in all species studied, highly expressed in connective tissue, various fibroblast and stromal cell lines and lastly, by tumor endothelial cell lines. In the mouse, CD90.2 is expressed on all thymocytes and peripheral T lymphocytes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Flow Cytometry

Panel Builder

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Lasp1 regulates adherens junction dynamics and fibroblast transformation in destructive arthritis.

Nature communications

Beckmann D,Römer-Hillmann A,Krause A,Hansen U,Wehmeyer C,Intemann J,de Gorter DJJ,Dankbar B,Hillen J,Heitzmann M,Begemann I,Galic M,Weinhage T,Foell D,Ai R,Kremerskothen J,Kiener HP,Müller S,Kamradt T,Schröder C,Leitão E,Horsthemke B,Rosenstiel P,Nordström K,Gasparoni G,Gasparoni N,Walter J,Li N,Yang X,Chung HR,Pavenstädt H,Lindemann N,Schnittler HJ,Wang W,Firestein GS,Pap T,Korb-Pap A

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 69-0902-82) in Immunohistochemistry

Tue Jun 15 00:00:00 EDT 2021

Therapeutic Potential of Mesenchymal Stem Cells in a Pre-Clinical Model of Diabetic Kidney Disease and Obesity.

International journal of molecular sciences

Sávio-Silva C,Soinski-Sousa PE,Simplício-Filho A,Bastos RMC,Beyerstedt S,Rangel ÉB

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 69-0902-82) in Flow Cytometry

Thu Feb 04 00:00:00 EST 2021

Cardiac fibroblast proliferation rates and collagen expression mature early and are unaltered with advancing age.

JCI insight

Wu R,Ma F,Tosevska A,Farrell C,Pellegrini M,Deb A

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 69-0902-82) in Flow Cytometry

Thu Dec 17 00:00:00 EST 2020

Loss of Fas signaling in fibroblasts impairs homeostatic fibrosis resolution and promotes persistent pulmonary fibrosis.

JCI insight

Redente EF,Chakraborty S,Sajuthi S,Black BP,Edelman BL,Seibold MA,Riches DW

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 69-0902-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2020

Partial characterization of the Sox2+ cell population in an adult murine model of digit amputation.

Tissue engineering. Part A

Agrawal V,Siu BF,Chao H,Hirschi KK,Raborn E,Johnson SA,Tottey S,Hurley KB,Medberry CJ,Badylak SF

Published figure using CD90.2 (Thy-1.2) monoclonal antibody (Product # 69-0902-82) in Flow Cytometry

Sun Jul 01 00:00:00 EDT 2012

生物信息学

蛋白别名: CD90; FLJ33325; Thy 1.2; Thy-1 antigen; Thy-1 membrane glycoprotein

基因别名: CD90; T25; Thy-1; Thy-1.2; Thy1; Thy1.1; Thy1.2

UniProt ID: (Mouse) P01831

Entrez Gene ID: (Mouse) 21838

分子生物学功能: cell adhesion molecule

功能

angiogenesis negative regulation of protein kinase activity cytoskeleton organization single organismal cell-cell adhesion negative regulation of cell migration positive regulation of GTPase activity retinal cone cell development protein autophosphorylation focal adhesion assembly negative regulation of axonogenesis T cell receptor signaling pathway negative regulation of T cell receptor signaling pathway positive regulation of T cell activation positive regulation of release of sequestered calcium ion into cytosol

参与通路

GTPase activator activity integrin binding enzyme binding protein kinase binding GPI anchor binding

CD90.2 (Thy-1.2) Monoclonal Antibody (53-2.1), eFluor™ 506, eBioscience™

产品信息

69-0902-82

免疫组化 (IHC)

流式细胞分析 (Flow)

种属反应

已发表种属

宿主/亚型

推荐的同型对照

分类

类型

克隆号

偶联物

激发/发射光谱

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

How to use the Panel Builder

生物信息学

性能保障

随时为您服务

Your items have has been added!