Invitrogen

CD90 (Thy-1) Monoclonal Antibody (eBio5E10 (5E10)), APC-eFluor™ 780, eBioscience™

CD90 (Thy-1) Antibody in Flow Cytometry (Flow)

图: 1 / 43

CD90 (Thy-1) Antibody (47-0909-42) in Flow

HEL cells were stained with Mouse IgG1 kappa Isotype Control, APC-eFluor 780 (Product # 47-4714-82) (blue histogram) or CD90 (Thy-1) Monoclonal Antibody, APC-eFluor 780 (purple histogram). Viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 450 (Product # 65-0863-18).

Product Image Gallery

产品信息

47-0909-42

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

5 µL (0.25 µg)/test

查看38篇已发表文章 38篇已发表文章

产品规格

种属反应

Human

已发表种属

Not Applicable

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

eBio5E10 (5E10)

偶联物

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

激发/发射光谱

756/785 nm 查看光谱

形式

Liquid

浓度

5 µL/Test

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2, with 0.2% BSA

内含物

0.09% sodium azide

保存条件

4° C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2848361

产品详细信息

Description: The eBio5E10 monoclonal antibody reacts with human CD90, also known as Thy-1 (thymus cell antigen-1). CD90 is a 25-35 kD receptor expressed on thymocytes, CD34+ prothymocytes, hematopoietic stem cells, neurons, a small subset of human fetal liver cells, cord blood cells, and bone marrow cells. CD90 is expressed on a subset of immature, CD34+ cells and a distinct subset of mature CD34- cells that are CD3+CD4+. The CD90+CD34+ population is enriched for cells capable of long-term culture. CD90 is involved in regulation of adhesion and signal transduction by T cells.

Applications Reported: This eBio5E10 (5E10) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBio5E10 (5E10) antibody has been pre-diluted and tested by flow cytometric analysis of human erythroleukemia (HEL) cells. This may be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

靶标信息

CD90 (Thy 1) antigen is a GPI linked glycoprotein member of the Immunoglobulin super family. CD90 is expressed in murine T cells, thymocytes, neural cells, Kupffer's cells and fibroblasts. CD90 may play a role in cell-cell or cell-ligand interactions during synaptogenesis and other events in the brain. CD90 can be used as a marker for a variety of stem cells and for the axonal processes of mature neurons. Structural study of CD90 led to the foundation of the Immunoglobulin superfamily, of which it is the smallest member, and led to the first biochemical description and characterization of a vertebrate GPI anchor. Diseases associated with CD90 dysfunction include nasopharyngeal carcinoma and thymoma.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.